Bone Marrow Stromal Cell Antigen 2 Is a Specific Marker of Type I IFN-Producing Cells in the Naive Mouse, but a Promiscuous Cell Surface Antigen following IFN Stimulation

Blasius, Amanda L.; Giurisato, Emanuele; Cella, Marina; Schreiber, Robert D.; Shaw, Andréy S.; Colonna, Marco

doi:10.4049/jimmunol.177.5.3260

Cited by 383 publications

(464 citation statements)

References 33 publications

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“…71,72 Notably, the specificity of BST-2 as a pDC marker was recently challenged, because BST-2 is inducible by interferon (IFN)-g treatment on other cells, including T cells, B cells and plasma cells. 70 The second pDC-restricted surface marker is Siglec-H, which is recognized by the 440C antibody that blocks type I IFN production by pDC, but does not induce depletion. 73 In contrast to the bulk of cDCs, pDCs are rather longlived and estimated to have an average lifespan of about 2 weeks.…”

Section: Pathogen Defensementioning

confidence: 99%

Probing in vivo dendritic cell functions by conditional cell ablation

Sapoznikov

2008

Immunol Cell Biol

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Dendritic cells (DCs) play a central role in T-cell activation and the control of the inherent autoreactivity of the T-cell compartment. Pleiotropic DC functions are likely associated with discrete DC subsets. However, the latter remain largely defined by phenotype and unique anatomic location, rather than function. The investigation of DC involvement in complex phenomena that rely on multicellular interactions, such as immuno-stimulation and tolerization calls for an assessment of DC functions within physiological context. Given the highly dynamic DC compartment, the method of choice to study in vivo DC functions is their conditional ablation in the intact organism. Here, we summarize the recent progress in this field highlighting pitfalls and prospects of the approach.

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Section: Pathogen Defensementioning

confidence: 99%

Probing in vivo dendritic cell functions by conditional cell ablation

Sapoznikov

2008

Immunol Cell Biol

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“…These two populations show a high degree of similarity with respect to function, development, and gene expression, and for simplicity will be grouped together and further referred to as CD4 + cDCs in this review [9]. A fourth population of plasmacytoid DCs (pDC) is defined by the expression of several distinguishing markers including B220, Siglec-H, and Bst2 [10][11][12]. Human counterparts for each of these DC subtypes have been identified, underscoring the conservation of DC lineage diversification across species, presumably owing to their important functional specialization [13][14][15].…”

Section: Unique Functions and Subsets Of DC Lineagesmentioning

confidence: 99%

Transcription factor networks in dendritic cell development

Satpathy

Murphy

2011

Seminars in Immunology

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Dendritic cells (DCs) are a heterogeneous population within the mononuclear phagocyte system (MPS) that derive from bone marrow precursors. Commitment and specification of hematopoietic progenitors to the DC lineage is critical for the proper induction of both immunity and tolerance. This review summarizes the important cytokines and transcription factors required for differentiation of the DC lineage as well as further diversification into specific DC subsets. We highlight recent advances in the characterization of immediate DC precursors arising from the common myeloid progenitor (CMP). Particular emphasis is placed on the corresponding temporal expression of relevant factors involved in regulating developmental options.

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“…The role of Tregs in a tumor microenvironment can be translated into anergy and immunosuppression, favoring the immune escape of tumor cells with the concomitant increase of tumor burden (14,22). To evaluate whether pDCs were crucial for tumor outgrowth after LPS treatment, we depleted pDCs using a pDC depleting Ab (m927 Ab) (14,23). m927 Ab or IgG control was injected the same day as LPS administration and every 2 d before mice were sacrificed on day 14 (7 d after LPS injection) (Fig.…”

Section: Low-dose Lps Facilitates An Immunosuppressive Environment Inmentioning

confidence: 99%

Plasmacytoid Dendritic Cells Play a Key Role in Tumor Progression in Lipopolysaccharide-Stimulated Lung Tumor–Bearing Mice

Rega

Terlizzi

Luciano

et al. 2013

The Journal of Immunology

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The antitumor activity of LPS was first described by Dr. William Coley. However, its role in lung cancer remains unclear. The aim of our study was to elucidate the dose-dependent effects of LPS (0.1–10 μg/mouse) in a mouse model of B16-F10–induced metastatic lung cancer. Lung tumor growth increased at 3 and 7 d after the administration of low-dose LPS (0.1 μg/mouse) compared with control mice. This was associated with an influx of plasmacytoid dendritic cells (pDCs), regulatory T cells, myeloid-derived suppressor cells, and CD8+ regulatory T cells. In contrast, high-dose LPS (10 μg/mouse) reduced lung tumor burden and was associated with a greater influx of pDCs, as well as a stronger Th1 and Th17 polarization. Depletion of pDCs during low-dose LPS administration resulted in a decreased lung tumor burden. Depletion of pDCs during high-dose LPS treatment resulted in an increased tumor burden. The dichotomy in LPS effects was due to the phenotype of pDCs, which were immunosuppressive after the low-dose LPS, and Th1- and T cytotoxic–polarizing cells after the high-dose LPS. Adoptive transfer of T cells into nude mice demonstrated that CD8+ T cells were responsible for pDC recruitment following low-dose LPS administration, whereas CD4+ T cells were required for pDC influx after the high-dose LPS. In conclusion, our data suggest differential effects of low-dose versus high-dose LPS on pDC phenotype and tumor progression or regression in the lungs of mice.

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Bone Marrow Stromal Cell Antigen 2 Is a Specific Marker of Type I IFN-Producing Cells in the Naive Mouse, but a Promiscuous Cell Surface Antigen following IFN Stimulation

Cited by 383 publications

References 33 publications

Probing in vivo dendritic cell functions by conditional cell ablation

Probing in vivo dendritic cell functions by conditional cell ablation

Transcription factor networks in dendritic cell development

Plasmacytoid Dendritic Cells Play a Key Role in Tumor Progression in Lipopolysaccharide-Stimulated Lung Tumor–Bearing Mice

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