Bone marrow stem cells for urologic tissue engineering

Shukla, Dave R.; Box, Geoffrey N.; Edwards, Robert A.; Tyson, Darren R.

doi:10.1007/s00345-008-0311-y

Cited by 84 publications

(54 citation statements)

References 23 publications

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“…The bone marrow-derived cells served as the source of undifferentiated cells that develop into smooth muscle cells. 20,[42][43][44][45] In this study, the tissue pores that were present 3 days after injury might have provided scaffolding and spaces suitable for colonization by the implanted bone marrow-derived cells. This would have optimized the chance for a high rate of cell survival and differentiation.…”

Section: Discussionmentioning

confidence: 99%

The Microenvironment of Freeze-Injured Mouse Urinary Bladders Enables Successful Tissue Engineering

Imamura

Yamamoto²,

Ishizuka³

et al. 2009

Tissue Engineering Part A

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Mouse bone marrow-derived cells implanted into freeze-injured bladder walls form smooth muscle layers, but not in intact walls. We determined if the microenvironment within injured urinary bladders was supportive of smooth muscle layer development. The urinary bladders of female nude mice were freeze-injured for 30 s. Three days later, the rate of blood flow in the wounded areas and in comparable areas of intact control urinary bladders was observed by charge-coupled device (CCD) video microscopy. Injured and control bladder walls were also analyzed histologically and cytologically. Growth factor mRNA expression was determined by real-time reverse transcription polymerase chain reaction arrays. The injured regions maintained a partial microcirculation in which blood flow velocity was significantly less than in controls. The injured bladder walls had few typical smooth muscle layers, and blood vessels in the walls had reduced smooth muscle content. The loss of smooth muscle cells in the bladder walls may have resulted in the formation of large porous spaces seen by scanning electron microscopy of the injured areas. The expression of nineteen growth-related mRNAs, including secreted phosphoprotein 1, inhibin b-A, glial cell line-derived neurotrophic factor, and transforming growth factor b1, were significantly upregulated in the injured urinary bladders. In conclusion, the microenvironment in freeze-injured urinary bladders enables successful tissue engineering.

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Section: Discussionmentioning

confidence: 99%

The Microenvironment of Freeze-Injured Mouse Urinary Bladders Enables Successful Tissue Engineering

Imamura

Yamamoto²,

Ishizuka³

et al. 2009

Tissue Engineering Part A

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“…The limited graft size in rodent models provides an inadequate model of regeneration due to the rate at which the regenerative process occurs. Small augmented areas tend to regenerate within a matter of weeks, whereas larger grafts, as seen in canine models, can take up to several months to regenerate completely [9,14,15]. More importantly, there exist anatomical differences between the lower nonhuman primates (NHPs) species and humans that include the musculature component of the lower urinary tract further questioning the translational validity of lower NHP models [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

A Nonhuman Primate Model for Urinary Bladder Regeneration Using Autologous Sources of Bone Marrow-Derived Mesenchymal Stem Cells

et al. 2011

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Animal models that have been used to examine the regenerative capacity of cell-seeded scaffolds in a urinary bladder augmentation model have ultimately translated poorly in the clinical setting. This may be due to a number of factors including cell types used for regeneration and anatomical/ physiological differences between lower primate species and their human counterparts. We postulated that mesenchymal stem cells (MSCs) could provide a cell source for partial bladder regeneration in a newly described nonhuman primate bladder (baboon) augmentation model. Cell-sorted2 baboon MSCs transduced with green fluorescent protein (GFP) were seeded onto small intestinal submucosa (SIS) scaffolds. Baboons underwent an approximate 40%-50% cystectomy followed by augmentation cystoplasty with the aforementioned scaffolds or controls and finally enveloped with omentum. Bladders from sham, unseeded SIS, and MSC/SIS scaffolds were subjected to trichrome, H&E, and immunofluorescent staining 10 weeks postaugmentation. Immunofluorescence staining for muscle markers combined with an anti-GFP antibody revealed that >90% of the cells were GFP Disclosure of potential conflicts of interest is found at the end of this article.

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“…MSCs have been isolated from bone marrow extracts in various animal species and established for in vivo or in vitro applications. Multipotent MSCs that had originally been identified by their ability to differentiate into mesenchymal lineages, such as osteogenic [20], chondrogenic [21,22] and marrow stromal lineages [23], have been considered to be promising tools for tissue engineering and cellular therapies [21,24,25]. In addition, MSCs have expanded in vitro without any apparent modification in phenotype or loss of multipotent function.…”

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confidence: 99%

Developmental Ability of Miniature Pig Embryos Cloned with Mesenchymal Stem Cells

Lee

Kang

Maeng

et al. 2010

Journal of Reproduction and Development

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Abstract. The present study compared the developmental ability of miniature pig embryos cloned with fetal fibroblasts (FFs), bone marrow-derived mesenchymal stem cells (MSCs) and differentiated (osteocytes, adipocytes and chondrocytes) MSCs. MSCs were isolated from an approximately 1-month-old female miniature pig (T-type, PWG Micro-pig ® , PWG Genetics Korea). MSCs were differentiated into osteocytes, adipocytes and chondrocytes under controlled conditions and characterized by cell surface antigen profile using specific markers. These differentiated or undifferentiated MSCs, as well as FFs of miniature pig, were transferred into enucleated oocytes of domestic pigs. Data from 10 replicates involving 1567 cloned embryos were assessed in terms of developmental rates. The in vitro development rate to the blastocyst stage of embryos cloned with undifferentiated MSCs was significantly (P<0.05) higher than that of embryos cloned with differentiated MSCs and FFs. Surgical transfer of 523 two-cell stage embryos cloned with undifferentiated MSCs into five synchronized domestic pig recipients resulted in 5 cloned miniature pig offspring (1 stillborn and 4 viable) from 2 pregnant recipients. The results imply that MSCs might be multipotent and that they can be used to produce viable cloned miniature pigs that cannot be easily reproduced with differentiated somatic cells. Key words: Animal cloning, Differentiation, Mesenchymal stem cells, Miniature pig, Nuclear transfer (J. Reprod. Dev. 56: [256][257][258][259][260][261][262] 2010) he production of cloned animals by nuclear transfer (NT) has been increasingly studied as a potential approach to tissue and organ xenotransplantation with reduced immunogenicity for endstage organ failure. Several studies have suggested that miniature pigs, rather than nonhuman-primate species, are suitable as a donor animal for human xenotransplantation [1,2] because miniature pigs are superior in terms of similarities in gross anatomy and physiology to humans as well as in terms of ethical issues. The NT efficiency, however, remains relatively low due to abnormalities throughout pre-and post-implantation development regardless of the species or type of donor cell [3]. A number of factors affect NT efficiency, including the NT technical process, activation protocol, recipient oocyte age, cell cycle stage and type of donor cells, which has been implicated in the precise reprogramming of chromatin and genomic imprinting. Among these factors, incomplete selection of donor cells has also been considered a prime cause for NT inefficiency.Transfer of embryonic stem (ES) cells to produce cloned embryos has resulted in consistently higher numbers of viable offspring compared with somatic cells in mice [4,5]. Moreover, embryos cloned with porcine mesenchymal stem cells (MSCs) and their derivatives along the osteogenic lineage give rise to an increase in the rate of preimplantation development compared with adult fibroblasts [6]. Our previous study on gene expression profiles demonstrated that some ge...

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Bone marrow stem cells for urologic tissue engineering

Cited by 84 publications

References 23 publications

The Microenvironment of Freeze-Injured Mouse Urinary Bladders Enables Successful Tissue Engineering

The Microenvironment of Freeze-Injured Mouse Urinary Bladders Enables Successful Tissue Engineering

A Nonhuman Primate Model for Urinary Bladder Regeneration Using Autologous Sources of Bone Marrow-Derived Mesenchymal Stem Cells

Developmental Ability of Miniature Pig Embryos Cloned with Mesenchymal Stem Cells

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