Bone marrow progenitors in inflammation and repair: new vistas in respiratory biology and pathophysiology

Denburg, Judah A.

doi:10.1183/09031936.06.00000706

Cited by 33 publications

(16 citation statements)

References 87 publications

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“…a Controls b Elastase/HGF BrdU-positive cells are also seen in bronchioli terminales (arrows) in both groups. a Controls b Elastase/ HGF granulocytes and monocytes changing to alveolar macrophages at the onset of emphysema induction [8]. This may be supported by the determined significant increase of the number of leucocytes in the blood and more inflammatory cells predominantly macrophages in the lung parenchyma.…”

Section: Discussionsupporting

confidence: 62%

Elastase-induced lung emphysema in rats is not reduced by hematopoietic growth factors when applied preventionally

et al. 2008

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We hypothesized that formation of pulmonary emphysema could be diminished after previous activation of stem cells. Animals received either a daily dose of the hematopoietic growth factors (GF; recombinant rat stem cell factor plus recombinant granulocyte colony stimulating factor; n=6, Elastase/GF group) or vehicle (n=9, Elastase/Sham group) starting 3 days before intratracheal instillation of elastase or vehicle and continued for another 25 days. Control animals were treated with NaCl (n=9, Sham/Sham group). On day 25, in all animals, a 2-mL pump was implanted subcutaneously that delivered 200 microg/h 5-bromo-2-desoxyuridine (BrdU) until study termination. Compared to controls, the Elastase/Sham group exhibited elevated total lung capacity (TLC) and functional residual capacity (FRC), significantly increased mean free alveolar pathway, alveolar volume, and decreased septal density. The Elastase/GF group showed (1) a significant increase of TLC and FRC, (2) a significant increase in alveolar size and volume, (3) a significant reduction of septal density, volume, and thickness. Proliferation in lung parenchyma and in terminal bronchioles remained significantly decreased in the Elastase/Sham group and the Elastase/GF group. Blood cell number has significantly increased in the Elastase/GF group. The application of GF-enhanced pulmonary emphysema, presumable because of increased inflammatory activity, was a result of a preventive treatment.

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Section: Discussionsupporting

confidence: 62%

Elastase-induced lung emphysema in rats is not reduced by hematopoietic growth factors when applied preventionally

et al. 2008

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“…These findings are probably reflecting a distinct route of exposure of the allergen in allergic rhinitis and are in line with previous studies which reported an increased frequency of basophils locally, within the airways, in allergic rhinitis [36,37]. Overall, these results further suggest that, in allergic rhinitis, the high-turnover state of the basophil progenitors, resulting the short span of these cells, could balance their levels in PB circulation [38]. …”

Section: Discussionsupporting

confidence: 91%

Impact of allergic rhinitis and specific subcutaneous immunotherapy on peripheral blood basophils of patients sensitized to Dermatophagoides pteronyssinus

Lopes¹,

Azenha²,

Teodósio

et al. 2013

All Asth Clin Immun

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BackgroundBasophils are important effectors cells in allergic rhinitis (AR) since they are involved in immunoglobulin (Ig) E – mediated inflammation and in the release of pro-inflammatory mediators. Specific subcutaneous immunotherapy (SCIT) provides clear immunologic modulation in some immune cells, however its systemic effects on basophils are not well known.MethodsPeripheral blood (PB) samples from 43 patients with allergic rhinitis mono-sensitized to Dermatophagoides pteronyssinus (Dpt) [33 of them under SCIT with allergoid Dpt extract, in maintenance dose (SCIT), with evaluation just before SCIT injection (SCIT-T0) and 4 hours later (SCIT-T4) and the other 10 Dpt allergic patients never having, in the past, undergone specific immunotherapy treatment (NSIT)], and 15 healthy age- and gender-matched controls (HG), were analyzed. For each sample, the total (t-IgE) and specific IgE (s-IgE) was performed, as well as, the relative frequency and absolute number of PB basophils and receptor-bound IgE and IgG expression were evaluated by flow cytometry and the Histamine N-methyltransferase (HNMT) and tryptase α/β1 (TPSAB1) gene expression was assessed by real-time PCR.ResultsHigher levels of receptor-bound IgE were observed in SCIT patients, which are correlated with the levels of serum t-IgE and s-IgE, whereas no significant differences were observed for receptor-bound IgG. Regarding HNMT mRNA expression, significantly lower expression levels were detected in AR patients compared to HG, independently of type of therapy. Moreover a negative correlation was found between HNMT gene expression and time under SCIT. Conversely, tryptase gene expression was significantly up-regulated in NSIT when compared to HG; however in SCIT patients, tryptase gene expression was significantly decreased than in NSIT patients. No differences were found for any parameter between SCIT-T0 and SCIT-T4 with exception of a transient increased expression of tryptase in SCIT-T4.ConclusionPB basophils from patients with AR show altered functional features, which seems to be influenced by SCIT, suggesting that these cells could be useful to clarify the SCIT triggered mechanisms at a systemic level.

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“…Our results support the research developed since 1985 by a Canadian group that sustains the involvement of the central immune system's organs, mainly bone marrow, in the pathophysiology of the allergic inflammation (Denburg et al, 1998). The results obtained in experimental models and in humans with allergy are essential, regarding the participation of hematopoietic mechanisms during the response to an allergenic (Denburg et al, 1998;Denburg & van Eeden, 2006, Sehmi et al, 1996Cyr & Denburg, 2001). In a comparative study with 153 patients with pollinic rhinoconjunctivitis, the effect of the administration of SIT either subcutaneously or intralymphatic was compared (Senti et al, 2008).…”

Section: Involvement Of the Organs From The Immune Systemsupporting

confidence: 73%

Specific Immunotherapy and Central Immune System

Pereira¹,

Loureiro²,

Tavares³

et al. 2012

Allergic Diseases - Highlights in the Clinic, Mechanisms and Treatment

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Bone marrow progenitors in inflammation and repair: new vistas in respiratory biology and pathophysiology

Cited by 33 publications

References 87 publications

Elastase-induced lung emphysema in rats is not reduced by hematopoietic growth factors when applied preventionally

Elastase-induced lung emphysema in rats is not reduced by hematopoietic growth factors when applied preventionally

Impact of allergic rhinitis and specific subcutaneous immunotherapy on peripheral blood basophils of patients sensitized to Dermatophagoides pteronyssinus

Specific Immunotherapy and Central Immune System

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