Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers

Abstract

Fibroblast colonies (clones) were obtained by explantation of bone marrow single-cell suspensions and were used to establish multicolony and single-colony derived fibroblast cultures by successive passaging of either pooled or individual colonies. When transpianted in diffusion chambers after 20-30 cell doublings in vitro, the descendants of fibroblast colony-forming cells (FCFC), whether grown from single or pooled colonies, retained the ability for bone and cartilage formation. The content of osteogenic precursors in the cultured progeny significantly outnumbered the initiating Correspondence : Professor A. J. Friedenstein.

Silk based biomaterials to heal critical sized femur defects

“…This is due to characteristics inherent in stem cells such as (i) a high proliferation capacity, (ii) ability for selfrenewal, (iii) multilineage differentiation and, therefore, potential for the repair of various tissues [48][49][50][51][52]. The cells used in this study were characterized as stem cells based on their surface markers as well as the multipotent and selective differentiation outcomes shown to bone or cartilage.…”

Identifying the Cellular Mechanisms Leading to Heterotopic Ossification

“…MSCs first isolated by Friedenstein et al (1987) were initially termed bone marrow-derived osteogenic stem cells due to their capacity to form cartilage and bone in vitro [27]. MSCs have frequently been shown to form endochondral bone when cultured under appropriate conditions (e.g.…”

Critical role for Y1 receptors in mesenchymal progenitor cell differentiation and osteoblast activity

“…Bone-forming osteoblasts, along with adipocytes and chondrocytes, are derived from mesenchymal stem cells (MSCs). (11,12) The study of MSCs has been hindered by their low abundance in vivo combined with a lack of specific cell surface markers to aid in their isolation. Therefore, since the 1970s, the ability of BMSCs to adhere to tissue-culture plastic has been used as a means of easily obtaining a population of cells that, under the appropriate culture conditions, can give rise to osteoblasts, (13) adipocytes, (14) chondrocytes, (15) and myoblasts.…”