Bone marrow mesenchymal stromal cell therapy for external urethral sphincter restoration in a rat model of stress urinary incontinence

Abstract

Rat MSCs have the ability to differentiate and skew their phenotype towards smooth and striated muscles, as demonstrated by SMA up-regulation and desmin expression. Periurethral injection of MSCs in an animal model of SUI restored the damaged external urethral sphincter and significantly improved VLPP.

Improvement of Anal Function by Adipose-Derived Stem Cell Sheets

“…ADSCs were cultured for 24 h in 3.5-cm dishes and then incubated in an adipogenic medium (DMEM-HG with 10% FBS, 0.5 mM isobutylmethylxanthine, 1 μM dexamethasone, 10 μM insulin, 200 μM indomethacin, and 1% penicillin-streptomycin [14]) or in myogenic medium (DMEM-HG with 2% FBS, 1% penicillin-streptomycin, and 1 ng/mL transforming factor-beta 1 [17]). As controls, ADSCs were grown in 3.5-cm dishes with complete media (without supplement).…”

Stem Cell Therapy for Stress Urinary Incontinence: A Critical Review

“…BMSCs have been used in 4 preclinical studies [46][47][48][49] but not in clinical studies ( Table 1). One of these studies used the SCs for seeding a degradable silk scaffold, which was then implanted as a sling in SUI rats [49].…”

“…The Crede technique, employed in 6 SC-SUI studies [19][20][21]46,49,63], is performed manually on an anesthetized animal by applying an increasing external force on the abdomen over the bladder with the experimenter's index finger. Immediately after a leak is visually observed at the urethral meatus, the experimenter lifts his/her finger off, and the peak intravesical pressure, that is, the LPP, is recorded via an intravesical catheter.…”

Histological and Urodynamic Effects of Autologous Stromal Vascular Fraction Extracted from Fat Tissue with Minimal Ex Vivo Manipulation in a Porcine Model of Intrinsic Sphincter Deficiency

“…These were essentially preclinical studies that used isolated muscle progenitor cells or ex vivo cultured MSCs from bone marrow (12), fat (13)(14)(15)(16) or cord blood (17). Four Phase I clinical studies have reported the efficacy of MSC in SUI, (18) in the context of a post-prostatectomy (19) feminine SUI after the failure of a first line pelvi-perineal physiotherapy or surgical treatment (20,21).…”