Bone Marrow Mesenchymal Stem Cells-Derived Exosomal MicroRNA-150-3p Promotes Osteoblast Proliferation and Differentiation in Osteoporosis

Qiu, Min; Zhai, Shuheng; Fu, Qin; Liu, Da

doi:10.1089/hum.2020.005

Cited by 65 publications

(47 citation statements)

References 32 publications

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“…Several miRNAs that participate in the differentiation of osteoblasts have been identified. For example, miR-497-5p (Zhao et al, 2020), microRNA-150-3p (Qiu et al, 2020), miR-708-5p (Wang et al, 2020), and microRNA-497-5p (Liu et al, 2020a) have been shown to promote this process, while miR-30 (Zhang et al, 2020a), miR-129-5p (Yin et al, 2020), miR-128-3p (Zhang et al, 2020b), and miR-17-5p have suppressive effects. In various cell types, miR-425-3p is differentially expressed (Li et al, 2020;Ma et al, 2019), but its function in osteogenesis has not been documented.…”

Section: Discussionmentioning

confidence: 99%

miR-452-3p inhibited osteoblast differentiation by targeting Smad4

Wang

Kong

et al. 2021

PeerJ

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Osteoblast differentiation is a complex process that is essential for normal bone formation. A growing number of studies have shown that microRNAs (miRNAs) are key regulators in a variety of physiological and pathological processes, including osteogenesis. In this study, BMP2 was used to induce MC3T3-E1 cells to construct osteoblast differentiation cell model. Then, we investigated the effect of miR-452-3p on osteoblast differentiation and the related molecular mechanism by RT-PCR analysis, Western blot analysis, ALP activity, and Alizarin Red Staining. We found that miR-452-3p was significantly downregulated in osteoblast differentiation. Overexpression miR-452-3p (miR-452-3p mimic) significantly inhibited the expression of osteoblast marker genes RUNX2, osteopontin (OPN), and collagen type 1 a1 chain (Col1A1), and decreased the number of calcium nodules and ALP activity. In contrast, knockdown miR-452-3p (miR-452-3p inhibitor) produced the opposite effect. In terms of mechanism, we found that Smad4 may be the target of miR-452-3p, and knockdown Smad4 (si-Smad4) partially inhibited the osteoblast differentiation enhanced by miR-452-3p. Our results suggested that miR-452-3p plays an important role in osteoblast differentiation by targeting Smad4. Therefore, miR-452-3p is expected to be used in the treatment of bone formation and regeneration.

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Section: Discussionmentioning

confidence: 99%

miR-452-3p inhibited osteoblast differentiation by targeting Smad4

Wang

Kong

et al. 2021

PeerJ

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“…BM-MSC-derived exosomal miR-150-3p could effectively promote osteoblast proliferation and differentiation in the ovariectomized (OVX) rat model of osteoporosis. 75 These findings provide new insights and motivation to exploit the naturally occurring exosomes for finding novel treatments of existing bone diseases.…”

Section: Introductionmentioning

confidence: 92%

Drug Delivery to the Bone Microenvironment Mediated by Exosomes: An Axiom or Enigma

Samal

Dash

2021

IJN

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The increasing incidence of bone-related disorders is causing a burden on the clinical scenario. Even though bone is one of the tissues that possess tremendous regenerative potential, certain bone anomalies need therapeutic intervention through appropriate delivery of a drug. Among several nanosystems and biologics that offer the potential to contribute towards bone healing, the exosomes from the class of extracellular vesicles are outstanding. Exosomes are extracellular nanovesicles that, apart from the various advantages, are standing out of the crowd for their ability to conduct cellular communication. The internal cargo of the exosomes is leading to its potential use in therapeutics. Exosomes are being unraveled in terms of the mechanism as well as application in targeting various diseases and tissues. Through this review, we have tried to understand and review all that is already established and the gap areas that still exist in utilizing them as drug delivery vehicles targeting the bone. The review highlights the potential of the exosomes towards their contribution to the drug delivery scenario in the bone microenvironment. A comparison of the pros and cons of exosomes with other prevalent drug delivery systems is also done. A section on the patents that have been generated so far from this field is included.

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“…Several studies proposed the presence of the mentioned miRANs in the exosomes derived from different sources of stem cells. Accordingly, the exosomes derived from BM-MSCs contained miR-29a ( Lu et al, 2020 ; Tan et al, 2020 ), miR-150 ( Qiu et al, 2021 ; Wu et al, 2021 ), miR-223 ( Chen L et al, 2018b ), miR-29a ( Su et al, 2019 ), and miR-125b ( Wang et al, 2019 ). miR-223 was also identified in the exosomes derived from umbilical cord MSCs ( Wei et al, 2020 ; Liu et al, 2021 ).…”

Section: The Application Of Stem Cell-derived Exosomes In Viral Diseasesmentioning

confidence: 99%