Bone Marrow Mesenchymal Stem Cells  Ameliorates Seawater-Exposure-Induced Acute Lung  Injury by Inhibiting Autophagy in Lung Tissue

Liu, Qiuping; Zhou, Daohong; Sun, Li; Luo, Lei; Wu, Changgui; Lin, Pu; Han, Shui-Ping

doi:10.1155/2014/104962

Cited by 15 publications

(19 citation statements)

References 22 publications

(39 reference statements)

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“…The hyperosmolar seawater has direct effect on the lung epithelial cells and causes the infiltration of inflammation cells into the alveolar spaces and releases various molecules, leading to lung edema and deterioration of gas exchange [3][4][5][6]15]. Proinflammatory cytokines, such as TNF-α and IL-6, are critical for initiating, amplifying, and perpetuating organ injury [5,6].…”

Section: Discussionmentioning

confidence: 99%

“…These pathological processes in turn can lead to alveolar epithelial-endothelial capillary barrier disruption, pulmonary edema, and severe refractory hypoxemia. Although many agents have been shown to attenuate seawater aspiration-induced ALI [4][5][6], alternative agents are needed to prevent or treat seawater aspiration-induced ALI.…”

Section: Discussionmentioning

confidence: 99%

“…Seawater aspiration represents one of the most important causes of acute lung injury (ALI), which is characterized by pulmonary edema and severe refractory hypoxemia [1][2][3][4]. In addition, the increased alveolar-capillary permeability caused by seawater aspiration allows the inflammatory cells infiltrate into the alveolar spaces and releases several molecules, such as pro-inflammatory cytokines and reactive oxygen species, leading to further pulmonary edema and deterioration of gas exchange [3,5,6].…”

Section: Introductionmentioning

confidence: 99%

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Erythropoietin Pretreatment Attenuates Seawater Aspiration-Induced Acute Lung Injury in Rats

Tong

Tan

et al. 2015

Inflammation

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Seawater drowning-induced acute lung injury (ALI) is a serious clinical condition characterized by increased alveolar-capillary permeability, excessive inflammatory responses, and refractory hypoxemia. However, current therapeutic options are largely supportive; thus, it is of great interest to search for alternative agents to treat seawater aspiration-induced ALI. Erythropoietin (EPO) is a multifunctional agent with antiinflammatory, antioxidative, and antiapoptotic properties. However, the effects of EPO on seawater aspiration-induced ALI remain unclear. In the present study, male rats were randomly assigned to the naive group, normal saline group, seawater group, or seawater + EPO group. EPO was administered intraperitoneally at 48 and 24 h before seawater aspiration. Arterial blood gas analysis was performed with a gas analyzer at baseline, 30 min, 1 h, 4 h, and 24 h after seawater aspiration, respectively. Histological scores, computed tomography scan, nuclear factor kappa B p65, inducible nitric oxide synthase, caspase-3, tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6, IL-10, wet-to-dry weight ratio, myeloperoxidase activity, malondialdehyde, and superoxide dismutase in the lung were determined 30 min after seawater aspiration. Our results showed that EPO pretreatment alleviated seawater aspiration-induced ALI, as indicated by increased arterial partial oxygen tension and decreased lung histological scores. Furthermore, EPO pretreatment attenuated seawater aspiration-induced increase in the expressions of pulmonary nuclear factor kappa B p65, inducible nitric oxide synthase, caspase-3, tumor necrosis factor-alpha, IL-1β, myeloperoxidase activity, and malondialdehyde when compared with the seawater group. Collectively, our study suggested that EPO pretreatment attenuates seawater aspiration-induced ALI by down-regulation of pulmonary pro-inflammatory cytokines, oxidative stress, and apoptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Erythropoietin Pretreatment Attenuates Seawater Aspiration-Induced Acute Lung Injury in Rats

Tong

Tan

et al. 2015

Inflammation

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“…13 Recent advances in mesenchymal stem cell (MSC) therapy have made this approach a strong candidate for clinical use in the treatment of several pulmonary diseases. [14][15][16][17] These multipotent nonhaematopoietic progenitors can be readily harvested from numerous tissues and expanded with high efficiency, and have strong immunosuppressive properties that can be exploited for successful autologous as well as heterologous transplantations. 18 Transplantation of MSCs has been reported in emphysemic rats, 14 murine asthma, 15 pulmonary hypertension in rats, 16 and acute lung injury in rats.…”

Section: Introductionmentioning

confidence: 99%

“…18 Transplantation of MSCs has been reported in emphysemic rats, 14 murine asthma, 15 pulmonary hypertension in rats, 16 and acute lung injury in rats. 17 This review article will discuss the biological effects and mechanisms of action of MSC transplantation in COPD.…”

Section: Introductionmentioning

confidence: 99%

Biological effects and mechanisms of action of mesenchymal stem cell therapy in chronic obstructive pulmonary disease

et al. 2015

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Chronic obstructive pulmonary disease (COPD) is the most frequent chronic respiratory disease and a leading cause of morbidity and mortality, worldwide. Given that the foremost risk factor leading to the development of COPD is cigarette smoke, the initial treatment for COPD is smoking cessation. Even after smoking cessation, inflammation, apoptosis and oxidative stress can persist and continue to contribute to COPD. Although current therapies for COPD (which are primarily based on anti-inflammatory drugs such as corticosteroids, theophylline and bronchodilators) reduce airway obstruction, limit COPD exacerbation and improve the patient's health-related quality-of-life, none can prevent disease progression or reduce mortality. Recent advances in stem cell research have provided novel insight into the potential of bone marrow mesenchymal stem cells (MSCs) in the treatment of several pulmonary diseases. This review article discusses the biological effects and mechanisms of action of MSC transplantation in COPD, and highlights the foundation that MSCs provide for novel therapeutic approaches in COPD.

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Bone marrow mesenchymal stem cells protect against n-hexane-induced neuropathy through beclin 1-independent inhibition of autophagy

Hao

Shi

et al. 2018

Sci Rep

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Chronic exposure to n-hexane, a widely used organic solvent in industry, induces central-peripheral neuropathy, which is mediated by its active metabolite, 2,5-hexanedione (HD). We recently reported that transplantation of bone marrow-mesenchymal stem cells (BMSC) significantly ameliorated HD-induced neuronal damage and motor deficits in rats. However, the mechanisms remain unclear. Here, we reported that inhibition of HD-induced autophagy contributed to BMSC-afforded protection. BMSC transplantation significantly reduced the levels of microtubule-associated protein 1 light chain 3-II (LC3-II) and the degradation of sequestosome-1 (p62) in the spinal cord and sciatic nerve of HD-intoxicated rats. Downregulation of autophagy by BMSC was also confirmed in VSC4.1 cells exposed to HD. Moreover, inhibition of autophagy by PIK III mitigated the neurotoxic effects of HD and, meanwhile, abolished BMSC-afforded neuroprotection. Furthermore, we found that BMSC failed to interfere with Beclin 1, but promoted activation of mammalian target of rapamycin (mTOR). Unc-like kinse 1 (ULK1) was further recognized as the downstream target of mTOR responsible for BMSC-mediated inhibition of autophagy. Altogether, BMSC transplantation potently ameliorated HD-induced autophagy through beclin 1-independent activation of mTOR pathway, providing a novel insight for the therapeutic effects of BMSC against n-hexane and other environmental toxicants-induced neurotoxicity.

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Bone Marrow Mesenchymal Stem Cells Ameliorates Seawater-Exposure-Induced Acute Lung Injury by Inhibiting Autophagy in Lung Tissue

Cited by 15 publications

References 22 publications

Erythropoietin Pretreatment Attenuates Seawater Aspiration-Induced Acute Lung Injury in Rats

Erythropoietin Pretreatment Attenuates Seawater Aspiration-Induced Acute Lung Injury in Rats

Biological effects and mechanisms of action of mesenchymal stem cell therapy in chronic obstructive pulmonary disease

Bone marrow mesenchymal stem cells protect against n-hexane-induced neuropathy through beclin 1-independent inhibition of autophagy

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