Bone Marrow Mesenchymal Stem Cell and Vein Conduit on Sciatic Nerve Repair in Rats

Abstract

Background:Peripheral nerve repair with sufficient functional recovery is an important issue in reconstructive surgery. Stem cells have attracted extensive research interest in recent years.Objectives:The purpose of this study was to compare the vein conduit technique, with and without the addition of mesenchymal stem cells in gap-less nerve injury repair in rats.Materials and Methods:In this study, 36 Wistar rats were randomly allocated to three groups: In the first group, nerve repair was performed with simple neurorrhaphy (control group), in the second group, nerve repair was done with vein conduit over site (vein conduit group) and in the third group, bone marrow stem cells were instilled into the vein conduit (stem cell group) after nerve repair with vein conduit over site. Six weeks after the intervention, the sciatic function index, electrophysiological study and histological examination were performed.Results:All animals tolerated the surgical procedures and survived well. The sciatic function index and latency were significantly improved in the vein conduit (P = 0.04 and 0.03, respectively) and stem cell group (P = 0.02 and 0.03, respectively) compared with the control group. No significant difference was observed in sciatic function and latency between the vein conduit and stem-cell groups. Moreover, histological analysis showed no significant difference in regenerative density between these two groups.Conclusions:The results of this study showed that the meticulous microsurgical nerve repair, which was performed using the vein tubulization induced significantly better sciatic nerve regeneration. However, the addition of bone marrow mesenchymal stem cell to vein conduit failed to promote any significant changes in regeneration outcome.

The advances in nerve tissue engineering: From fabrication of nerve conduit toin vivonerve regeneration assays

“…The conduit implantation investigations on the rat sciatic nerve use most often male rats weighing 200−250 g (Tao et al, ). Sprague Dawley (Hu et al, ; Zhuang et al, ) and Wistar rats (Abbasipour‐Dalivand, Mohammadi, & Mohammadi, ; Foroutan et al, ) are the most commonly used species, because articles analysis revealed that these strains are most commonly used in regeneration studies. The animals are kept in proper ventilation conditions at a suitable temperature (about 24 °C) and sufficient humidity (about 40−60%).…”

Donor nerve graft assessment for covering thumb nerve defects: a cadaveric study

“…When the injured nerve underwent a high tensile stress by simple microsurgical repair, the best technique for reconstruction is nerve graft or nerve transfer [14][15][16][17]. Although up to now, many techniques including conduits, veins, and allografts have been used to cover finger's nerve defects, the best is to do autologous nerve graft [18][19][20]. Autografts are superior to the allografts because they are genetically identical with the recipient tissue.…”

Mesenchymal stem cells in regenerative rehabilitation

“…Direct treatment of damaged peripheral nerves with MSCs has also been demonstrated to be effective. Numerous encouraging animal studies have shown that stem or progenitor cell treatments can rescue some degree of neurological function after injury 54 , 55 , 56 ) ( Fig. 1 ).…”

“… 55 ) Seyed Foroutan K et al Trauma Mon, 2015. 56 ) Lasso JM, et al J Plast Reconstr Aesthet Surg, 2015. 58 ) MSCs in the rehabilitation of damaged cartilage Repair of damaged cartilage, cartilage healing, efficient recovery of function, without significant complications.…”