Bone marrow mesenchymal stem cell sheets with high expression of hBD3 and CTGF promote periodontal regeneration

Wang, Li; Wei, Xinbo; Duan, Cui-mi; Yang, Jiarong; Xiao, Shengzhao; Liu, Haifeng; Sun, Jie

doi:10.1016/j.msec.2022.112657

Cited by 10 publications

(5 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Endothelial cells proliferate through in situ differentiation to form a preliminary vascular network structure. And the tube formation is an important indicator to analyze the pro-angiogenic ability of materials [ 38 ]. As expected, all the hydrogel groups showed obvious effects on tube formation and the CS/BSPA group appeared as the more effective one, which was probably attributed to the enhanced migration and proliferation of endothelial cells induced by BSP.…”

Section: Resultsmentioning

confidence: 99%

The utilization of chitosan/Bletilla striata hydrogels to elevate anti-adhesion, anti-inflammatory and pro-angiogenesis properties of polypropylene mesh in abdominal wall repair

Di,

Wang,

et al. 2024

Regenerative Biomaterials

View full text Add to dashboard Cite

Polypropylene (PP) mesh is commonly used in abdominal wall repair due to its ability to reduce the risk of organ damage, infections, and other complications. However, the PP mesh often leads to adhesion formation and does not promote functional tissue repair. In this study, we synthesized one kind of aldehyde Bletilla striata polysaccharide (BSPA) modified chitosan (CS) hydrogel based on Schiff base reaction. The hydrogel exhibited a porous network structure, a highly hydrophilic surface, and good biocompatibility. We wrapped the PP mesh inside the hydrogel and evaluated the performance of the resulting composites in a bilateral 1 × 1.5 cm abdominal wall defect model in rats. The results of gross observation, histological staining, and immunohistochemical staining demonstrated the positive impact of the CS hydrogel on anti-adhesion and wound healing effects. Notably, the addition of BSPA to the CS hydrogel further improved the performance of the composites in vivo, promoting wound healing by enhancing collagen deposition and capillary rearrangement. This study suggested that the BSPA-modified CS hydrogel significantly promoted the anti-adhesion, anti-inflammatory and pro-angiogenesis properties of PP meshes during the healing process. Overall, this work offers a novel approach to the design of abdominal wall repair patches.

show abstract

Section: Resultsmentioning

confidence: 99%

The utilization of chitosan/Bletilla striata hydrogels to elevate anti-adhesion, anti-inflammatory and pro-angiogenesis properties of polypropylene mesh in abdominal wall repair

Di,

Wang,

et al. 2024

Regenerative Biomaterials

View full text Add to dashboard Cite

show abstract

“…Previous studies have demonstrated that CTGF facilitated osteogenic differentiation of osteoblasts and periodontal regeneration. However, its effect on the cementogenesis of hPDLSCs is unknown, and the underlying mechanism is unclear [ 9 , 37 ]. Our experiments confirmed that CTGF reduced the resorption lacunae of the cementum root surface.…”

Section: Discussionmentioning

confidence: 99%

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Chen

et al. 2022

Stem Cell Res Ther

View full text Add to dashboard Cite

Background Orthodontic tooth movement inevitably induces cementum resorption, which is an urgent problem for orthodontists to confront. Human periodontal ligament stem cells (hPDLSCs) exert an important role in the orthodontic tooth movement and exhibit multidirectional differentiation ability in cementum regeneration. Connective tissue growth factor (CTGF) is an important extracellular matrix protein for bone homeostasis and cell differentiation. The purpose of our study was to explore the role of CTGF in cementum repair and cementogenesis and to elucidate its underlying mechanism. Methods A cementum defect model was established by tooth movement with heavy forces, and the cementum repair effect of CTGF was observed via micro-CT, HE staining and immunohistochemical staining. RT‒qPCR, western blotting (WB), alizarin red staining and ALP activity experiments verified the mineralization ability of hPDLSCs stimulated with CTGF. The expression of Cx43 in periodontal ligament cells was detected by WB and immunofluorescence (IF) experiments after CTGF stimulation in vivo and in vitro. Subsequently, the mineralization ability of hPDLSCs was observed after application of CTGF and the small interfering RNA Si-Cx43. Additionally, co-intervention via application of the small interfering RNA Si-CTGF and the Cx43 agonist ATRA in hPDLSCs was performed to deepen the mechanistic study. Next, WB, IF experiments and co-immunoprecipitation were conducted to confirm whether CTGF triggers the Cx43/β-catenin axis to regulate cementoblast differentiation of hPDLSCs. Results Local oral administration of CTGF to the cementum defects in vivo facilitated cementum repair. CTGF facilitated the cementogenesis of hPDLSCs in a concentration-dependent manner. Cx43 acted as a downstream effector of CTGF to regulate cementoblast differentiation. Si-Cx43 reduced CTGF-induced cementoblast differentiation. The Cx43 agonist ATRA restored the low differentiation capacity induced by Si-CTGF. Further mechanistic studies showed that CTGF triggered the activation of β-catenin in a dose-dependent manner. In addition, co-localization IF analysis and co-immunoprecipitation demonstrated that Cx43 interacted with β-catenin at cell‒cell connections. Si-Cx43 attenuated the substantial expression of β-catenin induced by CTGF. The Cx43 agonist reversed the inhibition of β-catenin induced by Si-CTGF. IF demonstrated that the nuclear importation of β-catenin was related to the immense expression of Cx43 at cell‒cell junctions. Conclusions Taken together, these data demonstrate that CTGF promotes cementum repair and cementogenesis through activation of the Cx43/β-catenin signalling axis.

show abstract

“…Xing et al 153 have also reported similar findings. Genetically modified cell sheets have also been explored as potential tools for use in the context of bone regeneration, 144,[154][155][156] although this strategy remains to be tested in human patients in a clinical setting, and further efforts are necessary to optimize this approach through the design of novel gene transfection approaches, efforts to control and prolong transgene expression, and other safety-related improvements. 157…”

Section: Cells Type Used For the Generation Of Cell Sheetsmentioning

confidence: 99%

Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration

You¹,

Lu²,

Li³

et al. 2022

IJN

View full text Add to dashboard Cite

Bone defects that are congenital or the result of infection, malignancy, or trauma represent a challenge to the global healthcare system. To address this issue, multiple research groups have been developing novel cell sheet technology (CST)-based approaches to promote bone regeneration. These methods hold promise for use in regenerative medicine because they preserve cellcell contacts, cell-extracellular matrix interactions, and the protein makeup of cell membranes. This review introduces the concept and preparation system of the cell sheet (CS), explores the application of CST in bone regeneration, highlights the current states of the bone regeneration via CST, and offers perspectives on the challenges and future research direction of translating current knowledge from the lab to the clinic.

show abstract

Bone marrow mesenchymal stem cell sheets with high expression of hBD3 and CTGF promote periodontal regeneration

Cited by 10 publications

References 56 publications

The utilization of chitosan/Bletilla striata hydrogels to elevate anti-adhesion, anti-inflammatory and pro-angiogenesis properties of polypropylene mesh in abdominal wall repair

The utilization of chitosan/Bletilla striata hydrogels to elevate anti-adhesion, anti-inflammatory and pro-angiogenesis properties of polypropylene mesh in abdominal wall repair

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration

Contact Info

Product

Resources

About