Bone marrow involvement in Q fever—detection by fluorine-18-labelled fluorodeoxyglucose PET

Alwis, Lankanatha; Balan, Kottekkattu; Wright, Paul A.; Lever, Andrew M. L.; Carmichael, Andrew

doi:10.1016/s1473-3099(09)70113-6

Cited by 15 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our aerosolization model, only SCID mice presented bone marrow lesions with diffuse macrophagic infiltrates, observed only from day 28. The bone marrow tropism of C. burnetii observed experimentally could fit with the hematophagocytic syndrome, ring granuloma, and hairy cell leukemia observed in humans (32)(33)(34)(35). Lymph node involvement during Q fever has recently been described in humans as lymphadenitis and lymphoma (36).…”

Section: Discussionsupporting

confidence: 63%

See 1 more Smart Citation

Mouse Model of Coxiella burnetii Aerosolization

et al. 2016

View full text Add to dashboard Cite

cCoxiella burnetii is mainly transmitted by aerosols and is responsible for multiple-organ lesions. Animal models have shown C. burnetii pathogenicity, but long-term outcomes still need to be clarified. We used a whole-body aerosol inhalation exposure system to mimic the natural route of infection in immunocompetent (BALB/c) and severe combined immunodeficient (SCID) mice. After an initial lung inoculum of 10 4 C. burnetii cells/lung, the outcome, serological response, hematological disorders, and deep organ lesions were described up to 3 months postinfection. C. burnetii-specific PCR, anti-C. burnetii immunohistochemistry, and fluorescent in situ hybridization (FISH) targeting C. burnetii-specific 16S rRNA completed the detection of the bacterium in the tissues. In BALB/c mice, a thrombocytopenia and lymphopenia were first observed, prior to evidence of C. burnetii replication. In all SCID mouse organs, DNA copies increased to higher levels over time than in BALB/c ones. Clinical signs of discomfort appeared in SCID mice, so follow-up had to be shortened to 2 months in this group. At this stage, all animals presented bone, cervical, and heart lesions. The presence of C. burnetii could be attested in situ for all organs sampled using immunohistochemistry and FISH. This mouse model described C. burnetii Nine Mile strain spread using aerosolization in a way that corroborates the pathogenicity of Q fever described in humans and completes previously published data in mouse models. C. burnetii infection occurring after aerosolization in mice thus seems to be a useful tool to compare the pathogenicity of different strains of C. burnetii.

show abstract

Section: Discussionsupporting

confidence: 63%

“…Its use to detect C. burnetii in human lymph nodes has only recently been published (34). Originally, we reported C. burnetiispecific 16S rRNA detection by FISH, reflecting the bacterium replication in a mouse model and presumably viable C. burnetii in the lung, liver, spleen, bone, and lymph nodes, enhancing immunohistochemistry findings.…”

Section: Discussionmentioning

confidence: 84%

Mouse Model of Coxiella burnetii Aerosolization

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Bone marrow involvement during Q fever has been reported in cases of pancytopenia, hemophagocytic syndrome with aspects of doughnut granuloma, [19,33] and has also recently been described as a diffuse bone marrow 18 F-FDG PET/CT hypermetabolism. [20,34] …”

Section: Discussionmentioning

confidence: 99%

18F-FDG PET/CT as a central tool in the shift from chronic Q fever to Coxiella burnetii persistent focalized infection

Eldin

Melenotte²,

Million

et al. 2016

Medicine

View full text Add to dashboard Cite

show abstract

“…PET scanning has been used for the identification of infectious foci in C. burnetii vascular infections (8,9,17) and in a case of Q fever in the bone marrow (18). PET scanning has higher sensitivity than computed tomography (CT) scanning for the evaluation of the extent and localization of infections (8,9,17).…”

Section: Discussionmentioning

confidence: 99%

Emergence of Q Fever Arthritis in France

et al. 2014

View full text Add to dashboard Cite

bOsteoarticular infection is an uncommon presentation of Q fever. Positron emission tomography (PET) scanning is a valuable tool for the diagnosis of Coxiella burnetii graft prosthesis infection and endocarditis. Our objective was to test a series of culture-negative osteoarticular samples using molecular assays for Coxiella burnetii. We tested for C. burnetii by molecular assays targeting the IS1111 and the IS30A spacer regions, using culture-negative osteoarticular samples obtained in our laboratory between January 2011and December 2012. We examine a total of 1,410 osteoarticular samples, and we observed two cases of arthritis and subacromial bursitis caused by C. burnetii. The infections were localized using PET scanning, and the diagnosis was confirmed through serology. For one, a C. burnetii strain with a multispacer sequence type 8 genotype was isolated from synovial fluid culture. Q fever articular infections could be undiagnosed because of the long evolution of articular attack, and patients with high antibody titers against C. burnetii should be tested using PET scanning to localize the site of infection.

show abstract

Bone marrow involvement in Q fever—detection by fluorine-18-labelled fluorodeoxyglucose PET

Cited by 15 publications

References 0 publications

Mouse Model of Coxiella burnetii Aerosolization

Mouse Model of Coxiella burnetii Aerosolization

18F-FDG PET/CT as a central tool in the shift from chronic Q fever to Coxiella burnetii persistent focalized infection

Emergence of Q Fever Arthritis in France

Contact Info

Product

Resources

About