Bone marrow graft as a source of allogeneic hematopoietic stem cells in patients undergoing a reduced intensity conditioning regimen

Abstract

In an attempt to reduce the incidence of chronic GVHD (cGVHD) after reduced-intensity conditioning (RIC), we used BM instead of PBSC and added melphalan 100 mg/m 2 to the classical association of fludarabine, 30 mg/m 2 /day for 3 days and TBI, 200 cGy (FLUIM regimen). Between 2000 and 2012, 51 patients received BM with the FLUIM regimen (group A), and 124 received BM (n = 22) or PBSC (n = 102) with another RIC regimen (group B). Donors were siblings (n = 123) or HLA-matched 10/10 unrelated (n = 52). Full donor-type chimerism at day 100 was more often recorded in group A (86%) than in group B (62%); P o0.001. There was no difference between the two groups in terms of OS and EFS, acute GVHD, relapse and non-relapse mortality incidence. cGVHD occurred more often in group B (41%) than in group A (23%); P = 0.021. In multivariate analysis, the two risk factors associated with the development of cGVHD were conditioning in group B (hazard ratio (HR) = 2.871, 95% confidence interval (CI) (1.372-6.006); P = 0.005) and CD34 + count (HR = 1.009, 95% CI (1.006-1.011); P o 0.001). In conclusion, the FLUIM regimen followed by BM leads to more frequent full-donor chimerism and a reduced incidence of cGVHD without compromising relapse and survival.