Bone Marrow derived stem cell subpopulations that show specific homing abilities

Abstract

Bone marrow derived stem cells (BMSCs) are able to generate many different cell types. Understanding biological significance and molecular mechanisms underlying adult stem cell trafficking seems crucial. Currently though, the real potential of BMSCs is not yet fully accepted and data reported after injection in uninjured animals are contradictory. This may be due to the use of total BMSCs, which may contain at variable rate and percentage different subpopulations with specific homing properties.We isolated from the BM of C57B1/6‐Tg(UBC‐GFP)30Scha/J adult mice, 4 different populations according to molecular markers or culture methods: CD105+, MSC, floaters (D. Trisler's method) and buffy coat. After extensive immunophenotyping and microarray analysis, they have been injected in the tail vein of C57B1/6 wildtype mice. Animals have been sacrificed 2 days later and different tissues harvested. In each tissue, the presence of GFP+ cells has been analyzed by PCR and immunohistochemistry. Here we present data indicating not only specific homing of the subpopulations in different tissues, but even preferential localization within each tissue.