Bone marrow-derived SP cells can contribute to the respiratory tract of mice in vivo

MacPherson, Heather; Keir, Pamela A; Webb, Shaun; Samuel, Kay; Boyle, Shelagh; Bickmore, Wendy A.; Forrester, Lesley M.; Dorin, Julia R.

doi:10.1242/jcs.02375

Cited by 81 publications

(73 citation statements)

References 36 publications

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“…2B, i, Table I, and data not shown). Other authors have also shown that not all male cells are positive for the Y chromosome by FISH, which may reflect the plane of sectioning of individual nuclei (17,18). Control female tracheal sections from female mice did not demonstrate any hybridization with the Y-labeled probe (Fig.…”

Section: Male Ck5 ϩ Circulating Progenitor Epithelial Cells Contributmentioning

confidence: 75%

Circulating Progenitor Epithelial Cells Traffic via CXCR4/CXCL12 in Response to Airway Injury

Gomperts

Belperio²,

Rao

et al. 2006

The Journal of Immunology

132

120

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Recipient airway epithelial cells are found in human sex-mismatched lung transplants, implying that circulating progenitor epithelial cells contribute to the repair of the airway epithelium. Markers of circulating progenitor epithelial cells and mechanisms for their trafficking remain to be elucidated. We demonstrate that a population of progenitor epithelial cells exists in the bone marrow and the circulation of mice that is positive for the early epithelial marker cytokeratin 5 (CK5) and the chemokine receptor CXCR4. We used a mouse model of sex-mismatched tracheal transplantation and found that CK5+ circulating progenitor epithelial cells contribute to re-epithelialization of the airway and re-establishment of the pseudostratified epithelium. The presence of CXCL12 in tracheal transplants provided a mechanism for CXCR4+ circulating progenitor epithelial cell recruitment to the airway. Depletion of CXCL12 resulted in the epithelium defaulting to squamous metaplasia, which was derived solely from the resident tissue progenitor epithelial cells. Our findings demonstrate that CK5+CXCR4+ cells are markers of circulating progenitor epithelial cells in the bone marrow and circulation and that CXCR4/CXCL12-mediated recruitment of circulating progenitor epithelial cells is necessary for the re-establishment of a normal pseudostratified epithelium after airway injury. These findings support a novel paradigm for the development of squamous metaplasia of the airway epithelium and for developing therapeutic strategies for circulating progenitor epithelial cells in airway diseases.

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Section: Male Ck5 ϩ Circulating Progenitor Epithelial Cells Contributmentioning

confidence: 75%

Circulating Progenitor Epithelial Cells Traffic via CXCR4/CXCL12 in Response to Airway Injury

Gomperts

Belperio²,

Rao

et al. 2006

The Journal of Immunology

132

120

View full text Add to dashboard Cite

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“…Recent in vitro studies suggest that marrow stromal stem cells can contribute to the airway epithelium (4). Also, a recent in vivo study suggests that freshly isolated BM cells can contribute to the respiratory tract (23).…”

Section: Discussionmentioning

confidence: 99%

Assessment of cystic fibrosis transmembrane conductance regulator (CFTR) activity in CFTR-null mice after bone marrow transplantation

Bruscia¹,

Price²,

Cheng³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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Several studies have demonstrated that bone marrow (BM)-derived cells give rise to rare epithelial cells in the gastrointestinal (GI) and respiratory tracts after BM transplantation into myeloablated recipients. We investigate whether, after transplantation of cystic fibrosis transmembrane conductance regulator (CFTR)-positive BM-derived cells, BM-derived GI and airway epithelial cells can provide CFTR activity in the GI tract and nasal epithelium of recipient cystic fibrosis mice. CFTR؊͞؊ mice were transplanted with wild-type BM after receiving different doses of irradiation, and CFTR activity was assessed in vivo in individual mice over time by using rectal and nasal potential difference analyses and in vitro by Ussing chamber analysis. The data suggest that rare BM-derived epithelial cells in the GI and nasal epithelium detected in CFTR؊͞؊ transplanted mice provide a modest level of CFTR-dependent chloride secretion. Detection of CFTR mRNA and protein in tissues of transplanted CFTR؊͞؊ mice supports these data.bone marrow stem cells ͉ chloride channel ͉ epithelial cells

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“…[49][50][51] To address if embryonic-like stem Embryonic-like stem cells in adult bone marrow M Kucia et al cells identified by us are present in SP of BMMNC, we isolated from BM a side population of BMMNC (Figure 7 panel a). For comparison, we also isolated from the same marrow samples Sca-1 þ lin À CD45 À cells (Figure 5a).…”

Section: Sca-1 þ Lin à Cd45 à Vsel Cells Are Present In the Side Popumentioning

confidence: 99%

A population of very small embryonic-like (VSEL) CXCR4+SSEA-1+Oct-4+ stem cells identified in adult bone marrow

et al. 2006

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By employing multiparameter sorting, we identified in murine bone marrow (BM) a homogenous population of rare (B0.02% of BMMNC) Sca-1 þ lin À CD45 À cells that express by RQ-PCR and immunohistochemistry markers of pluripotent stem cells (PSC) such as SSEA-1, Oct-4, Nanog and Rex-1. The direct electronmicroscopical analysis revealed that these cells are small (B2-4 lm), posses large nuclei surrounded by a narrow rim of cytoplasm, and contain open-type chromatin (euchromatin) that is typical for embryonic stem cells. In vitro cultures these cells are able to differentiate into all three germ-layer lineages. The number of these cells is highest in BM from young (B1-monthold) mice and decreases with age. It is also significantly diminished in short living DBA/2J mice as compared to long living B6 animals. These cells in vitro respond strongly to SDF-1, HGF/SF and LIF and express CXCR4, c-met and LIF-R, respectively, and since they adhere to fibroblasts they may be coisolated with BM adherent cells. We hypothesize that this population of Sca-1 þ lin À CD45 À very small embryonic-like (VSEL) stem cells is deposited early during development in BM and could be a source of pluripotent stem cells for tissue/ organ regeneration.

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Bone marrow-derived SP cells can contribute to the respiratory tract of mice in vivo

Cited by 81 publications

References 36 publications

Circulating Progenitor Epithelial Cells Traffic via CXCR4/CXCL12 in Response to Airway Injury

Circulating Progenitor Epithelial Cells Traffic via CXCR4/CXCL12 in Response to Airway Injury

Assessment of cystic fibrosis transmembrane conductance regulator (CFTR) activity in CFTR-null mice after bone marrow transplantation

A population of very small embryonic-like (VSEL) CXCR4+SSEA-1+Oct-4+ stem cells identified in adult bone marrow

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