Bone Marrow-Derived Multipotent Stromal Cells Attenuate Inflammation in Obliterative Airway Disease in Mouse Tracheal Allografts

Casey, Alicia; Dirks, Fabian; Liang, Olin D.; Harrach, Hakima; Schuette-Nuetgen, Katharina; Leeman, Kristen T.; Kim, Carla F.; Gérard, Craig; Subramaniam, Meera

doi:10.1155/2014/468927

Cited by 13 publications

(7 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…33 To date, several cell sources have been suggested including pluripotent stem cells, human umbilical mesenchymal stem cells, urothelial, and smooth muscle cells. [34][35][36][37] In our studies for enhancing the integrative properties of grafts, we applied allogenic BMSCs which have immunosuppressive action [17][18][19] and are able to differentiate in different directions. [20][21][22] The data obtained clearly demonstrate the efficacy and safety of a two-layer scaffold (PL-SF) seeded with allogenic BMSCs in the reconstruction of the bladder wall in a model of partial cystectomy in rabbits.…”

Section: Discussionmentioning

confidence: 99%

“…13,14 To increase the biocompatibility of the synthesized graft we applied bone marrow stromal cells (BMSCs) that were previously shown to enhance bladder regeneration. 15,16 BMSCs have immunosuppressive effects, [17][18][19] high proliferative potential and the ability to differentiate into different tissue lines [20][21][22] including cells of the urothelium.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Experimental bladder regeneration using a poly-L-lactide/silk fibroin scaffold seeded with nanoparticle-labeled allogenic bone marrow stromal cells

Yudintceva¹,

Nashchekina²,

Блинова³

et al. 2016

IJN

View full text Add to dashboard Cite

In the present study, a poly- l -lactide/silk fibroin (PL-SF) bilayer scaffold seeded with allogenic bone marrow stromal cells (BMSCs) was investigated as a potential approach for bladder tissue engineering in a model of partial bladder wall cystectomy in rabbits. The inner porous layer of the scaffold produced from silk fibroin was designed to promote cell proliferation and the outer layer produced from poly- l -lactic acid to serve as a waterproof barrier. To compare the feasibility and efficacy of BMSC application in the reconstruction of bladder defects, 12 adult male rabbits were divided into experimental and control groups (six animals each) that received a scaffold seeded with BMSCs or an acellular one, respectively. For BMSC tracking in the graft in in vivo studies using magnetic resonance imaging, cells were labeled with superparamagnetic iron oxide nanoparticles. In vitro studies demonstrated high intracellular incorporation of nanoparticles and the absence of a toxic influence on BMSC viability and proliferation. Following implantation of the graft with BMSCs into the bladder, we observed integration of the scaffold with surrounding bladder tissues (as detected by magnetic resonance imaging). During the follow-up period of 12 weeks, labeled BMSCs resided in the implanted scaffold. The functional activity of the reconstructed bladder was confirmed by electromyography. Subsequent histological assay demonstrated enhanced biointegrative properties of the PL-SF scaffold with cells in comparison to the control graft, as related to complete regeneration of the smooth muscle and urothelium tissues in the implant. Confocal microscopy studies confirmed the presence of the superparamagnetic iron oxide nanoparticle-labeled BMSCs in newly formed bladder layers, thus indicating the role of stem cells in bladder regeneration. The results of this study demonstrate that application of a PL-SF scaffold seeded with allogenic BMSCs can enhance biointegration of the graft in vivo and support bladder tissue regeneration and function.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Experimental bladder regeneration using a poly-L-lactide/silk fibroin scaffold seeded with nanoparticle-labeled allogenic bone marrow stromal cells

Yudintceva¹,

Nashchekina²,

Блинова³

et al. 2016

IJN

View full text Add to dashboard Cite

show abstract

“…However, they can also have profibrotic effects. ere are multiple studies with animal models using MSCs to treat BOS [94][95][96][97][98]. Cao et al showed that in seven patients with BOS and respiratory failure, six of them presented with lung function improvement 6 months after they were given i.v.…”

Section: Efficacy Of Bronchodilator Treatmentmentioning

confidence: 99%

Postinfectious Bronchiolitis Obliterans in Children: Diagnostic Workup and Therapeutic Options: A Workshop Report

Jerkic

Brinkmann

Calder

et al. 2020

Canadian Respiratory Journal

Self Cite

View full text Add to dashboard Cite

Bronchiolitis obliterans (BO) is a rare, chronic form of obstructive lung disease, often initiated with injury of the bronchiolar epithelium followed by an inflammatory response and progressive fibrosis of small airways resulting in nonuniform luminal obliteration or narrowing. The term BO comprises a group of diseases with different underlying etiologies, courses, and characteristics. Among the better recognized inciting stimuli leading to BO are airway pathogens such as adenovirus and mycoplasma, which, in a small percentage of infected children, will result in progressive fixed airflow obstruction, an entity referred to as postinfectious bronchiolitis obliterans (PIBO). The present knowledge on BO in general is reasonably well developed, in part because of the relatively high incidence in patients who have undergone lung transplantation or bone marrow transplant recipients who have had graft-versus-host disease in the posttransplant period. The cellular and molecular pathways involved in PIBO, while assumed to be similar, have not been adequately elucidated. Since 2016, an international consortium of experts with an interest in PIBO assembles on a regular basis in Geisenheim, Germany, to discuss key areas in PIBO which include diagnostic workup, treatment strategies, and research fields.

show abstract

“…This production is then associated with elevations of IL-6 inducible reactants CRP and LPS-binding protein. [127]. An observational study of pre-term infants who developed sepsis noted that these infants had decreased monocyte class II antigen, again suggesting that the adaptive immune system is delayed [128].…”

Section: Profile Of Pediatric Innate and Adaptive Immunitymentioning

confidence: 99%

The Host Immune Response to Tissue-Engineered Organs: Current Problems and Future Directions

Wiles¹,

Fishman²,

Coppi³

et al. 2016

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

As the global health burden of chronic disease increases, end-stage organ failure has become a costly and intractable problem. De novo organ creation is one of the long-term goals of the medical community. One of the promising avenues is that of tissue engineering: the use of biomaterials to create cells, structures, or even whole organs. Tissue engineering has emerged from its nascent stage, with several proof-of-principle trials performed across various tissue types. As tissue engineering moves from the realm of case trials to broader clinical study, three major questions have emerged: (1) Can the production of biological scaffolds be scaled up accordingly to meet current and future demands without generating an unfavorable immune response? (2) Are biological scaffolds plus or minus the inclusion of cells replaced by scar tissue or native functional tissue? (3) Can tissue-engineered organs be grown in children and adolescents given the different immune profiles of children? In this review, we highlight current research in the immunological response to tissue-engineered biomaterials, cells, and whole organs and address the answers to these questions.

show abstract

Bone Marrow-Derived Multipotent Stromal Cells Attenuate Inflammation in Obliterative Airway Disease in Mouse Tracheal Allografts

Cited by 13 publications

References 44 publications

Experimental bladder regeneration using a poly-L-lactide/silk fibroin scaffold seeded with nanoparticle-labeled allogenic bone marrow stromal cells

Experimental bladder regeneration using a poly-L-lactide/silk fibroin scaffold seeded with nanoparticle-labeled allogenic bone marrow stromal cells

Postinfectious Bronchiolitis Obliterans in Children: Diagnostic Workup and Therapeutic Options: A Workshop Report

The Host Immune Response to Tissue-Engineered Organs: Current Problems and Future Directions

Contact Info

Product

Resources

About