Bone marrow-derived mesenchymal stem cells modulate BV2 microglia responses to lipopolysaccharide

Ooi, Yin Yin; Ramasamy, Rajesh; Rahmat, Zul ' atfi; Subramaiam, Hemavathy; Tan, Shi Wei; Abdullah, Maha; Israf, Daud Ahmad; Vidyadaran, Sharmili

doi:10.1016/j.intimp.2010.09.001

Cited by 48 publications

(33 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the precise polarization states of microglia in the presence of MSCs under physiological or inflammatory conditions remain largely unknown. In most studies, xenogenic experimental systems were used; that is, cocultures of human MSCs with mouse or rat MGs [26,[31][32][33] and primary microglia were frequently replaced with neoplastic cell lines (BV2 or N9) [34][35][36] or with hippocampal slices [37]. Only two laboratories used autologous mouse [35] or rat [38] primary MGs and MSCs.…”

Section: Au3 Cmentioning

confidence: 99%

Regulation of Mouse Microglia Activation and Effector Functions by Bone Marrow-Derived Mesenchymal Stem Cells

Hegyi

Környei

Ferenczi

et al. 2014

Stem Cells and Development

View full text Add to dashboard Cite

Section: Au3 Cmentioning

confidence: 99%

Regulation of Mouse Microglia Activation and Effector Functions by Bone Marrow-Derived Mesenchymal Stem Cells

Hegyi

Környei

Ferenczi

et al. 2014

Stem Cells and Development

View full text Add to dashboard Cite

“…There were also numerous studies using MSC as a therapeutic tool in various models of neuroinflammation [27,28,29]; MSC demonstrate immunomodulatory effects on microglia [15,16,17,18]. In addition, several studies have shown that MSC-CM accelerates wound healing [30,31], promotes liver regeneration [32] and improves cardiac function following myocardial infarction [33].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, MSC show potential for immunotherapy for neurodegenerative diseases as they have been shown to modulate microglial activation [15,16]. Several studies have demonstrated the immunomodulatory effects of MSC on microglial in coculture systems involving cell-cell interactions [15,17,18]. However, there are more obstacles and complex effects produced with cell transplantation in the CNS than in other parts of the body [19,20].…”

Section: Introductionmentioning

confidence: 99%

Paracrine Effects of Mesenchymal Stem Cells-Conditioned Medium on Microglial Cytokines Expression and Nitric Oxide Production

Ooi

Dheen

Tay³

2014

Neuroimmunomodulation

Self Cite

View full text Add to dashboard Cite

Background/Aim: Microglia, the resident macrophages in the central nervous system, secrete various proinflammatory cytokines and undergo proliferation upon activation in various neurodegenerative diseases. Activation of microglia has been implicated in exacerbation of various neurodegenerative diseases. Recently, it has been proposed that mesenchymal stem cells (MSC) have immunosuppressive properties and the potential to moderate inflammation. This study aimed to elucidate the effects of MSC-conditioned medium (MSC-CM) in modulating microglial activation by analyzing microglial proinflammatory and anti-inflammatory factors [interleukin (IL)-6, tumor necrosis factor (TNF)-a, inducible nitric oxide synthase (iNOS) and IL-10], signaling pathway molecules [NFκB, c-Jun N-terminal kinase (JNK) and MKP-1) and NO production. Methods: Immortalized murine microglia cell line, BV2 microglia and primary microglia isolated from C57BL/6 mouse pup brains were used in this study. Mouse MSC were isolated from the male C57BL/6 mouse tibia and fibula. The effects of MSC-CM on the expression of inflammatory cytokines and signaling molecules in microglia were elucidated using RT-PCR, immunofluorescence analysis and Western blot analysis. NO production in microglia was assessed using a Griess kit. Results: MSC-CM significantly reduced the mRNA and protein expression levels of proinflammatory cytokines (IL-6 and TNF-a) in microglia activated by lipopolysaccharide (LPS). In addition, MSC-CM significantly reduced the protein expression of NFκB, JNK and c-Jun, but increased the expression levels of IL-10 and MKP-1 in activated BV2 microglia. NO production and iNOS expression by BV2 microglia in MSC-CM were increased. Conclusions: Overall, our findings suggest that MSC immunomodulate microglial activities through paracrine effects.

show abstract

“…Conversely, MSC from Balb/c took longer to differentiate into osteocytes compared to the other 2 strains tested in our study. In view of the duration that MSC cultures remained in suitable phenotype and their ease to differentiation, our group has primarily utilised ICR mice for downstream experimentation where we show these stem cells to have immunomodulatory properties [13] . MSC from all 3 different strains of mice tested were suitable sources for bone marrow-derived MSC as they showed typical morphology, immunophenotype and differentiation capacities of MSC.…”

Section: Discussionmentioning

confidence: 99%

“…The long term self-renewal and multilineage differentiation properties of MSC reflect their potential for tissue regeneration and cell/gene therapybased treatment of diseases [6] including acute graft-vs-host disease [7] , osteogenesis imperfecta [8] , cardiomyopathy [9] and Crohn's disease [10] . Recent findings that MSC have immunosuppressive properties [11][12][13][14] have also increased the need for a suitable supply of MSC for experimental research. The expansion of undifferentiated MSC is a research tool for functional and genetic studies for subsequent development of preclinical protocols to treat a wide range of diseases.…”

Section: Brief Articlementioning

confidence: 99%

Immunophenotype and differentiation capacity of bone marrow-derived mesenchymal stem cells from CBA/Ca, ICR and Balb/c mice

Ooi

Rahmat²,

Jose³

et al. 2013

WJSC

Self Cite

View full text Add to dashboard Cite

AIM:To assess the capacity to isolate and expand mesenchymal stem cells (MSC) from bone marrow of CBA/Ca, ICR and Balb/c mice. METHODS:Bone marrow of tibia and femur were flushed, cultured and maintained in supplemented Dulbecco's modified Eagle's medium. MSC immunophenotype of cultures were tracked along increasing passages for positivity to CD106, Sca-1 and CD44 and negativity to CD45, CD11b and MHC class Ⅱ. Differentiation capacity of MSC towards osteogenic and adipogenic lineages were also assessed. RESULTS

show abstract

Bone marrow-derived mesenchymal stem cells modulate BV2 microglia responses to lipopolysaccharide

Cited by 48 publications

References 72 publications

Regulation of Mouse Microglia Activation and Effector Functions by Bone Marrow-Derived Mesenchymal Stem Cells

Regulation of Mouse Microglia Activation and Effector Functions by Bone Marrow-Derived Mesenchymal Stem Cells

Paracrine Effects of Mesenchymal Stem Cells-Conditioned Medium on Microglial Cytokines Expression and Nitric Oxide Production

Immunophenotype and differentiation capacity of bone marrow-derived mesenchymal stem cells from CBA/Ca, ICR and Balb/c mice

Contact Info

Product

Resources

About