Bone marrow-derived macrophages from BALB/c and C57BL/6 mice fundamentally differ in their respiratory chain complex proteins, lysosomal enzymes and components of antioxidant stress systems

Depke, Maren; Breitbach, Katrin; Dang, Khoa Dinh Hoang; Brinkmann, Lars; Salazar, Manuela Gesell; Dhople, Vishnu M.; Bast, Antje; Steil, Leif; Schmidt, Frank; Steinmetz, Ivo; Völker, Uwe

doi:10.1016/j.jprot.2014.03.027

Cited by 26 publications

(20 citation statements)

References 50 publications

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“…There are well known differences in immune and physiological processes between C57Bl/6J and Balb/cJ mice, including stress that is well known to modulate gastric emptying . Blood corticosterone levels were tested as a quantitative measure of stress from three groups of mice: baseline C57Bl/6J mice that did not undergo gastric emptying and C57Bl/6J and Balb/cJ mice that underwent gastric emptying.…”

Section: Resultsmentioning

confidence: 99%

“…Four hours after ingestion, the GI tract was removed from C57Bl/6J (n = 9) and Balb/cJ (n = 12) mice and the percent of total tracer was calculated for each of the five gastrointestinal regions ( Figure 4A). There was a significant difference in the distri- There are well known differences in immune and physiological processes between C57Bl/6J and Balb/cJ mice, including stress [31][32][33] that is well known to modulate gastric emptying. 34 Blood corticosterone levels were tested as a quantitative measure of stress from three groups of mice: baseline C57Bl/6J mice that did not undergo gastric emptying and C57Bl/6J and Balb/cJ mice that underwent gastric emptying.…”

Section: Strain Differencesmentioning

confidence: 99%

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High temporal resolution gastric emptying breath tests in mice

Miller

Bajzer

Hein

et al. 2018

Neurogastroenterology Motil

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Section: Resultsmentioning

confidence: 99%

Section: Strain Differencesmentioning

confidence: 99%

High temporal resolution gastric emptying breath tests in mice

Miller

Bajzer

Hein

et al. 2018

Neurogastroenterology Motil

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show abstract

“…As Gusb-deficient BMDCs were capable of activating Dectin-2-reporter cells, other protein(s) are likely to provide the same particular glycans to be recognized by Dectin-2. Given that Gusb is one of the most abundant proteins in myeloid cells [22] but not lymphocytes (data not shown), more detail analysis using sensitive Mass spectrometry may identify other “career” proteins which are less abundantly expressed. Although the precise glycan structure on Gusb has not been determined yet, our data suggest that mannose is likely to be involved in the recognition by Dectin-2 (Fig 1F).…”

Section: Discussionmentioning

confidence: 99%

C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells

Mori¹,

Shibata²,

Yamasaki³

2017

PLoS ONE

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C-type lectin receptors (CLRs) recognize pathogen-derived ligands and abnormal self that trigger protective immune responses. However, the precise nature of self ligands recognized by CLRs remains to be determined. Here, we found that Dectin-2 recognizes bone marrow-derived dendritic cells (BMDCs) using Dectin-2-expressing reporter cells. This activity was inhibited by an excessive amount of mannose, and by the mutation of mannose-binding motif in Dectin-2. β-glucuronidase (Gusb) was identified as a protein bound to Dectin-2 and mutations of N-glycosylation sites in Gusb impaired the binding of Gusb to Dectin-2. Overexpression of Gusb in a macrophage cell line conferred an ability to stimulate Dectin-2-expressing reporter cells. Our study suggests that a glycosylated protein with mannose-related structure is recognized by Dectin-2.

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“…By far, more data are available from viral [86,87,88,89,90] and bacterial [91,92,93,94,95] pathogens.…”

Section: Interaction Of Macrophages With Human Pathogenic Fungimentioning

confidence: 99%

Challenges and Strategies for Proteome Analysis of the Interaction of Human Pathogenic Fungi with Host Immune Cells

et al. 2015

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Opportunistic human pathogenic fungi including the saprotrophic mold Aspergillus fumigatus and the human commensal Candida albicans can cause severe fungal infections in immunocompromised or critically ill patients. The first line of defense against opportunistic fungal pathogens is the innate immune system. Phagocytes such as macrophages, neutrophils and dendritic cells are an important pillar of the innate immune response and have evolved versatile defense strategies against microbial pathogens. On the other hand, human-pathogenic fungi have sophisticated virulence strategies to counteract the innate immune defense. In this context, proteomic approaches can provide deeper insights into the molecular mechanisms of the interaction of host immune cells with fungal pathogens. This is crucial for the identification of both diagnostic biomarkers for fungal infections and therapeutic targets. Studying host-fungal interactions at the protein level is a challenging endeavor, yet there are few studies that have been undertaken. This review draws attention to proteomic techniques and their application to fungal pathogens and to challenges, difficulties, and limitations that may arise in the course of simultaneous dual proteome analysis of host immune cells interacting with diverse morphotypes of fungal pathogens. On this basis, we discuss strategies to overcome these multifaceted experimental and analytical challenges including the viability of immune cells during co-cultivation, the increased and heterogeneous protein complexity of the host proteome dynamically interacting with the fungal proteome, and the demands on normalization strategies in terms of relative quantitative proteome analysis.

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Bone marrow-derived macrophages from BALB/c and C57BL/6 mice fundamentally differ in their respiratory chain complex proteins, lysosomal enzymes and components of antioxidant stress systems

Cited by 26 publications

References 50 publications

High temporal resolution gastric emptying breath tests in mice

High temporal resolution gastric emptying breath tests in mice

C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells

Challenges and Strategies for Proteome Analysis of the Interaction of Human Pathogenic Fungi with Host Immune Cells

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