Bone Marrow Cells Immunomagnetically Selected For CD271+ Antigen Promote<i>In Vitro</i>the Repair of Articular Cartilage Defects

Hermida‐Gómez, Tamara; Fuentes‐Boquete, Isaac; Gimeno-Longas, Maria José; Muiños‐López, Emma; Díaz‐Prado, Silvia; Toro, Javier de; Blanco, Francisco J.

doi:10.1089/ten.tea.2010.0346

Cited by 45 publications

(37 citation statements)

References 40 publications

(50 reference statements)

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“…CD271 has been strongly linked to chondrogenesis in several studies. When MSCs were isolated with conventional plastic adhesion, the CD271+ portion was superior to the CD271-portion of MSCs in cartilage matrix deposition, tissue formation and integration with the native cartilage in an in vitro cartilage repair model [Hermida-Gómez et al, 2011]. In human synovium, CD271+ cells are more chondrogenic than CD73+ and CD106+ subpopulations [Arufe et al, 2010].…”

Section: Discussionmentioning

confidence: 97%

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

Watson

Foo

et al. 2013

Cells Tissues Organs

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CD271 has been applied to isolate mesenchymal stem cells (MSCs) from bone marrow and other tissues. Umbilical cord blood is a unique resource of stem cells and endothelial progenitor cells. Isolation of MSCs from umbilical cord blood, however, has been inefficient and inconsistent. This study was designed to examine the potential application of CD271 as a marker for the isolation of MSCs from umbilical cord blood. CD271+ cells were isolated from umbilical cord blood and bone marrow using CD271 antibody-conjugated microbeads, and characterized in osteogenic, chondrogenic and adipogenic differentiation. CD271+ cells from umbilical cord blood were slow to proliferate compared with those isolated from bone marrow. While CD271+ cells from bone marrow differentiated into osteogenic, chondrogenic and adipogenic lineages, there were no sound indications of differentiation by CD271+ cells from umbilical cord blood under the same differentiation conditions applied to the CD271+ cells from bone marrow. The study also found that bone marrow CD271+ cells remarkably upregulated the expression of chondrogenic genes under chondrogenic differentiation induction. When implanted into bone defects in mice, CD271+ cells from bone marrow regenerated significant bone, but the counterparts in umbilical cord blood formed little bone in the bone defects. In conclusion, CD271 is an efficient marker for MSC isolation from bone marrow but has failed to isolate MSCs from umbilical cord blood. CD271+ cells in bone marrow are particularly chondrogenic. The property of CD271+ cells is unique but varies from different tissues.

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Section: Discussionmentioning

confidence: 97%

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

Watson

Foo

et al. 2013

Cells Tissues Organs

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“…These properties hold MSCs as a promising candidate for the development of new therapeutics for the application in regenerative medicine [Kode et al, 2009] and tissue engineering in order to accelerate wound healing [Maxson et al, 2012] and for articular cartilage treatment [Hermida-Gómez et al, 2011]. It has been proven that MSCs can be harvested from many human tissues, such as the bone marrow [Prockop, 1997;Caplan and Bruder, 2001], adipose tissue [Zuk et al, 2001] and skin [Toma et al, 2005;Lorenz et al, 2008].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of CD271<sup>+</sup> Stem Cells Derived from Sheep Dermal Skin

Jahroomishirazi¹,

Bader²,

Ebert³

et al. 2014

Cells Tissues Organs

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Background: Mesenchymal stem cells (MSCs) have great promise in the field of regenerative medicine due to their differentiation potential into several lineages. Besides the bone marrow, MSCs can be obtained from the dermis, which represents a large stem cell reservoir in the skin. Sheep provide an appropriate large animal model for preclinical studies. In this study, we focused on the isolation and characterization of MSCs from sheep dermis as an alternative to bone marrow MSCs (bmMSCs). Methods: Primary ovine cells were obtained from the dermis for comparison with bone marrow. CD271⁺/45^- dermal MSCs (CD271-dMSCs), which were sorted by flow cytometry, and plastic-adherent bmMSCs were examined for morphology, proliferation and senescence-associated β-galactosidase activity in both low and high oxygen conditions. CD271 expression on cultured cells was assessed by flow cytometry. Adipogenic and osteogenic potentials of CD271-dMSCs were evaluated by oil red O and von Kossa staining. Chondrogenic capacity of CD271-dMSCs and CD271⁺/CD45^- bone marrow cells (CD271-bmMSCs) was detected using immunohistochemistry and measurement of sulfated glycosaminoglycans. Results: The cell proliferation assay demonstrated no significant difference between CD271-dMSCs and bmMSCs under low oxygen conditions. Cultured CD271-dMSCs revealed much more CD271 expression compared to CD271-bmMSCs. CD271-dMSCs and CD271-bmMSCs showed basically similar expression of the cartilage-specific proteins aggrecan and collagen type II, although with a stronger staining in CD271-bmMSC-derived cultures. Remarkably, there was co-expression of CD271 and aggrecan during chondrogenic differentiation, suggesting an involvement of CD271 in chondrogenesis. Conclusion: Based on these findings, CD271-dMSCs might serve as an appropriate alternative cell source in preclinical research.

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“…Since the CD271 antigen is able to promote cell proliferation and differentiation along three mesenchymal lineages, i.e. adipogenic, osteogenic and chondrogenic [27,28,[38][39][40], it has been intensely investigated as an antigen driving generation of differentiated cells suitable for specific therapeutic applications. Obtaining the chondrocyte-like phenotype in an attempt to regenerate cartilage has been one of these experimental directions.…”

Section: Discussionmentioning

confidence: 99%

“…Obtaining the chondrocyte-like phenotype in an attempt to regenerate cartilage has been one of these experimental directions. Because it was found that the CD271 + MSCs can effectively differentiate towards cartilage tissue [15,27,28], we decided to check if these cells can differentiate into chondrocyte-like cells of the nucleus pulposus (NP) of IVD. However, since CD271 is a very efficient nerve growth factor receptor, its presence may lead to nerve ingrowth into NP.…”

Section: Discussionmentioning

confidence: 99%

“…The finding that the CD271 + MSCs can effectively differentiate towards cartilage tissue [15,27,28] has inspired us to investigate the ability of these cells to differentiate into chondrocyte-like cells of the nucleus pulposus (NP) of IVD. We have also investigated CD271 -MSCs and compared these cells to the entire population of MSCs generated by plastic adherence [29][30][31] …”

Section: Cd271 Mscs In Ivd Regenerationmentioning

confidence: 99%

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The feasibility of the CD271+ and CD271– mesenchymal stromal cell enrichment toward nucleus pulposus-like cells

Jezierska-Woźniak

Barczewska

Habich

et al. 2017

Folia Histochem Cytobiol.

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introduction. Factors promoting nerve cell ingrowth are considered responsible for chronic back pain resulting from the intervertebral disc degeneration (IDD). One of the recent exploratory IDD treatments is stem cell transplantation therapy. The CD271 (low-affinity nerve growth factor receptor) has been identified as a marker of the most homogeneous mesenchymal stem cell (MSC) subset. It is capable of promoting differentiation along adipogenic, osteogenic and chondrogenic lineages and producing significantly higher levels of cytokines as compared to the total population of plastic adherence-mesenchymal stem cells (PA-MSCs). We investigated the ability of CD271 + MSCs to differentiate into chondrocyte-like cells of the nucleus pulposus (NP) of intervertebral disc. We also examined CD271 -MSCs, using PA-MSCs as a control cell population. Material and methods. Bone marrow derived PA-MSCs and its two subsets, CD271 -MSCs and CD271 + MSCs, were seeded in collagen scaffolds. After two weeks of growth in NP-differentiation medium, RNA was isolated from cells-scaffold constructs and was analyzed by q-PCR for expression of NP markers. Glycosaminoglycans were analyzed biochemically directly in cells-scaffold constructs. results. Expression of NP markers -extracellular matrix components such as aggrecan, collagen type II and glycosaminoglycans on both RNA and the protein levels -was significantly higher in CD271-MSCs compared to the CD271 + MSCs and PA-MSCs cell populations. Conclusions. CD271-MSCs may be superior candidates for NP restorative treatment compared to CD271 + MSCs and PA-MSCs due to their ability of expressing NP-supporting extracellular matrix components at levels higher than the other two studied MSC subsets.

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Bone Marrow Cells Immunomagnetically Selected For CD271+ Antigen PromoteIn Vitrothe Repair of Articular Cartilage Defects

Cited by 45 publications

References 40 publications

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

Isolation and Characterization of CD271<sup>+</sup> Stem Cells Derived from Sheep Dermal Skin

The feasibility of the CD271+ and CD271– mesenchymal stromal cell enrichment toward nucleus pulposus-like cells

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