Bone Marrow as Source of Cells in Reactions of Cellular Hypersensitivity

Lubaroff, David M.; Waksman, Byron H.

doi:10.1084/jem.128.6.1425

Cited by 93 publications

(13 citation statements)

References 10 publications

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“…2). Although it has been demonstrated that the bone marrow in the rabbit serves as a prime source of antigen-reactive cells (4,5) and macrophages (46)(47)(48), and that in the rodent the cell mediating humoral and cellular immunity appears to originate in the bone marrow (25,(49)(50)(51), the organ(s) of origin of the antibody-forming cell(s) in the rabbit still remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

Cells Involved in the Immune Response

Richter

Abdou

1969

The Journal of Experimental Medicine

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Bone marrow cells obtained from rabbits of one allotype were injected into irradiated rabbits of a different allotype. The recipients were also injected with sheep red blood cells, and their spleen cells were tested for plaque-forming capacity 7 days later. Spleen cells of all recipients gave large numbers of plaques as did spleen cells incubated with antiserum, directed toward donor allotype. However, incubation of the recipient spleen cells with antiserum directed toward recipient allotype completely suppressed plaque formation. These results demonstrate that antibody-formation in irradiated recipients of transferred lymphoid cells is a property of the recipient animal and that the antibody-forming cell is relatively irradiation-resistant. It was also demonstrated that only viable normal bone marrow cells are capable of transferring antibody-forming capacity to irradiated recipient rabbits. Neither sonicates nor heat-killed preparations of normal rabbit bone marrow cells possessed this capacity.

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Section: Discussionmentioning

confidence: 99%

Cells Involved in the Immune Response

Richter

Abdou

1969

The Journal of Experimental Medicine

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“…Failure to detect a lymnphocyte defect Lubaroff and Waksman (1968) showed that the capacity to develop a DHS reaction to PPD could be adoptively transferred to non-immune rats with intravenously administered lymphoid cells from B.C.G. immunized rats.…”

Section: Sequestration Of Macrophages In Growing Tumoursmentioning

confidence: 99%

Anti-tumour Activity of Aprotinin

Latner¹,

Longstaff²,

Turner³

1974

Br J Cancer

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Summary.-The effects of rat tumours of various macrophage contents on the syngeneic host's ability to produce either: (1) an inflammatory exudate in response to intraperitoneal oyster glycogen -or (2) a cutaneous delayed hypersensitivity (DHS) response to PPD or SRBC after appropriate sensitization, were studied as a function of tumour growth.Both these reactions were found to be markedly decreased as the tumours grew. The suppression was greatest in animals bearing tumours of high macrophage content. The suppression of the DHS response could be reversed by a local injection of normal peritoneal macrophages with the eliciting antigen, and lymphocytes from tumour bearing animals exhibiting poor DHS responses were able to adoptively transfer DHS reactivity to normal unsensitized recipients. The monocyte infiltration in response to oyster glycogen was also decreased, and these data indicate a monocyte, rather than a lymphocyte defect in the tumour induced " anergy " in this system.

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“…It was shown by Volkman and Gowans (21) and by Spector et al (22) that macrophages in inflammatory infiltrates stem from blood monocytes produced in the bone marrow, and others have shown that delayed-hypersensitivity reactions develop poorly or not at all in the absence of bone marrowderived cells (23,24). The importance of these cells in the present system was clearly demonstrated in experiments where recipients were X-irradiated with 800 R on the day of production of the heat lesions 5 days before transfer of cells from adrenal or spinal cord-sensitized donors.…”

Section: (19 20)mentioning

confidence: 99%

The Nature and the Specificity of Mononuclear Cells in Experimental Autoimmune Inflammations and the Mechanisms Leading to Their Accumulation

Werdelin

McCluskey

1971

The Journal of Experimental Medicine

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The nature and specificity of the mononuclear cells in passively transferred autoimmune encephalomyelitis and adrenalitis were studied. The recipients were prepared by production of a small heat lesion in the target tissue 5 days before transfer. Within 24 hr after transfer of lymph node cells from donors sensitized with the corresponding tissue antigen, a dense mononuclear cell infiltrate developed around the lesion. When lymph node cells labeled in vitro with 3H-thymidine or 3H-adenosine were transferred, a significant number of labeled lymphocytes was found in the infiltrate at 24 or 48 hr. Lymphocytes labeled with 3H-thymidine showed a greater tendency to accumulate than cells labeled with 3H-adenosine, indicating that newly formed lymphocytes were more prone to enter the reaction than older cells. Labeled lymphocytes and macrophages of recipient origin and labeled lymphocytes from donors stimulated with B. pertussis were also shown to accumulate around the heat lesion provided the reaction had been initiated by transfer of unlabeled lymphocytes from donors sensitized to the appropriate tissue-specific antigen. In recipients which were given lymph node cells from two groups of donors, sensitized either to spinal cord or to adrenal antigens, with cells from only one group of donors labeled, equal percentages of labeled cells were found around each lesion. Thus, no evidence of preferential accumulation of specifically sensitized lymphocytes was obtained. In recipients which received whole body irradiation on the day of production of the heat lesions, 5 days before transfer of lymph node cells from appropriately sensitized donors, neither monocytes nor lymphocytes accumulated around the lesion. However, if the tibial bone marrow was shielded or if bone marrow cells were given to the recipients shortly after irradiation, inflammation developed as in normal recipients. In recipients which were irradiated 24 hr after the transfer of unlabeled lymph node cells from donors sensitized to the appropriate tissue antigen and then given labeled lymph node cells from B. pertussis-stimulated donors, labeled lymphocytes were found in the reaction 24 hr later. This accumulation occurred although virtually all the lymphocytes present in the lesion at 24 hr after the first transfer were destroyed by the irradiation. The results are interpreted as follows. The autoimmune reaction is initiated by the arrival at the site of a few specifically sensitized lymphocytes, probably on a random basis. After contact with antigen, factors are produced and released which cause the influx of monocytes and of lymphocytes, in particular newly formed ones, of various specificities. There is no preferential accumulation of specifically sensitized cells. The influx of lymphocytes appears to require the presence of monocytes or macrophages in the reaction.

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Bone Marrow as Source of Cells in Reactions of Cellular Hypersensitivity

Cited by 93 publications

References 10 publications

Cells Involved in the Immune Response

Cells Involved in the Immune Response

Anti-tumour Activity of Aprotinin

The Nature and the Specificity of Mononuclear Cells in Experimental Autoimmune Inflammations and the Mechanisms Leading to Their Accumulation

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