Bone marrow- and subcutaneous adipose tissue-derived mesenchymal stem cells: Differences and similarities

Abstract

Bone marrow (BM) and subcutaneous adipose tissue (Ad) are both considered being prospective sources of MSC for therapeutic applications. However, functional properties and therapeutic efficacy of MSC derived from different tissues of the same patient are still poorly investigated. In our study, BM-MSC and F-MSC cultures from 43 adult donors were evaluated in successive passages for immunophenotype, secretion of VEGF, SDF1, MCP1, IL6 and TGFβ1, frequency of colony-forming units (CFU-F), frequency of adipo- and osteo-progenitors (CFU-Ad, CFU-Ost), and for onset of in vitro replicative senescence. We have demonstrated that at early passages (P2-P4 or up to 14-15 in vitro population doublings) BM- and Ad- derived MSC cultures are comparable in such important characteristics as proliferation rate (population doubling time: 3.4±0.2% in BM-MSC, 3±0.3% in F-MSC), clonogenity (CFU-F frequency: 32±5% in BM-MSC, 31±5% in F-MSC), differentiation potential (CFU-Ad frequency: 10.4±2% in BM-MSC, 13±3% in F-MSC; CFU-Ost frequency: 18.5±5.5% in BM-MSC, 18±5% in F-MSC), but differ significantly in abundance of CD146⁺ fraction within the sample (25±5% in BM-MSC, 7±3% in F-MSC) and in a level of VEGF, SDF-1, MCP1 and TGFβ1 secretion. We have also demonstrated that BM-MSC enter senescence after P3-4 while most of F-MSC did not show senescence features up to P6-8. Together, these data demonstrate that specific properties of MSC from different sources should be always taken into account, when developing and optimizing the specific protocols for MSC expansion and evaluation for each particular clinical application.

Low power laser irradiation and human adipose-derived stem cell treatments promote bone regeneration of critical-sized calvarial defects in rats

“…The results are consistent with many preceding studies providing robust scientific evidence on the osteogenic potency of ADSCs and their ability to promote bone repair and regeneration in vivo [40][41][42][43][44]. ADSCs have great potential for bone regeneration due to their many appreciated features, including their increased accessibility and resistance to senescence and malignant transformation [45][46][47]. The secretome of the ADSCs contains several endocrine factors with bone remodeling activity [48][49][50].…”

WISP1 Is a Novel Adipokine Linked to Inflammation in Obesity

“…Human mesenchymal stem cells were isolated from abdominal SAT samples (25) and differentiated into mature adipocytes in DMEM-F12 supplemented with 500 …”

Cranioplasty with Adipose-Derived Stem Cells, Beta-Tricalcium Phosphate Granules and Supporting Mesh: Six-Year Clinical Follow-Up Results

“…In recent years, ASCs have been under extensive study in regenerative medicine 15, 16, 17, 18. They are easily retrieved in high numbers from fat tissue and expanded in vitro, which makes them an attractive tool in tissue reconstruction 14, 19, 20.…”