Bone Marrow Adipose Tissue Quantification by Imaging

Hassan, Ebrahim Bani; Ghasem‐Zadeh, Ali; Imani, Mahdi; Kutaiba, Numan; Wright, David K.; Sepehrizadeh, Tara; Duque, Gustavo

doi:10.1007/s11914-019-00539-5

Cited by 14 publications

(8 citation statements)

References 97 publications

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“…The mid-thigh was defined as midway between the tip of the greater trochanter and medial edge of the tibial plateau, located by deep palpation. The images were exported and further analyzed by Slice-O-Matic ™ (Tomovision, Montreal, CA) to determine the muscle, intramuscular fat and subcutaneous fat volumes as previously described [31][32][33]. After visual checks; where due to beam hardening artefacts the tissue was not segmented ("tagged") optimally, the assignment of individual voxels or small voxel islands were changed into the correct tissue manually, at the discretion of the operator.…”

Section: Peripheral Quantitative Computed Tomography (Pqct)mentioning

confidence: 99%

Intermittent fasting and continuous energy restriction result in similar changes in body composition and muscle strength when combined with a 12 week resistance training program

et al. 2022

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Purpose The objective of this study was to compare the effects of 12 weeks of resistance training combined with either 5:2 intermittent fasting or continuous energy restriction on body composition, muscle size and quality, and upper and lower body strength. Methods Untrained individuals undertook 12 weeks of resistance training plus either continuous energy restriction [20% daily energy restriction (CERT)] or 5:2 intermittent fasting [~ 70% energy restriction 2 days/week, euenergetic consumption 5 days/week (IFT)], with both groups prescribed a mean of ≥ 1.4 g of protein per kilogram of body weight per day. Participants completed 2 supervised resistance and 1 unsupervised aerobic/resistance training combination session per week. Changes in lean body mass (LBM), thigh muscle size and quality, strength and dietary intake were assessed. Results Thirty-four participants completed the study (CERT = 17, IFT = 17). LBM was significantly increased (+ 3.7%, p < 0.001) and body weight (− 4.6%, p < 0.001) and fat (− 24.1%, p < 0.001) were significantly reduced with no significant difference between groups, though results differed by sex. Both groups showed improvements in thigh muscle size and quality, and reduced intramuscular and subcutaneous fat assessed by ultrasonography and peripheral quantitative computed tomography (pQCT), respectively. The CERT group demonstrated a significant increase in muscle surface area assessed by pQCT compared to the IFT group. Similar gains in upper and lower body strength and muscular endurance were observed between groups. Conclusion When combined with resistance training and moderate protein intake, continuous energy restriction and 5:2 intermittent fasting resulted in similar improvements in body composition, muscle quality, and strength. ACTRN: ACTRN12620000920998, September 2020, retrospectively registered.

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Section: Peripheral Quantitative Computed Tomography (Pqct)mentioning

confidence: 99%

Intermittent fasting and continuous energy restriction result in similar changes in body composition and muscle strength when combined with a 12 week resistance training program

et al. 2022

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“…Dr. Duque discussed secreted factors that couple bone marrow adipocytes to the surrounding skeletal cells and their implications for the pathophysiology of osteoporosis. In addition, he provided information about key technical approaches that can be used to study aspects of bone marrow adipocyte function and lipotoxicity both in vitro and ex vivo (19,20). After this, Dr. Kerckhofs demonstrated how novel contrast agents such as polyoxometalate can be used to visualize and to quantify bone marrow adipocytes in three dimensions, in addition to relating this information to bone and vascular quantifications using high-resolution computed tomography (21).…”

Section: Session Iv: Advanced Methods For Clinical and Pre-clinical Assessment Of Bone Marrow Adiposity And Skeletal Healthmentioning

confidence: 99%

Report From the 6th International Meeting on Bone Marrow Adiposity (BMA2020)

2021

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The 6th International Meeting on Bone Marrow Adiposity (BMA) entitled “Marrow Adiposity: Bone, Aging, and Beyond” (BMA2020) was held virtually on September 9th and 10th, 2020. The mission of this meeting was to facilitate communication and collaboration among scientists from around the world who are interested in different aspects of bone marrow adiposity in health and disease. The BMA2020 meeting brought together 198 attendees from diverse research and clinical backgrounds spanning fields including bone biology, endocrinology, stem cell biology, metabolism, oncology, aging, and hematopoiesis. The congress featured an invited keynote address by Ormond MacDougald and ten invited speakers, in addition to 20 short talks, 35 posters, and several training and networking sessions. This report summarizes and highlights the scientific content of the meeting and the progress of the working groups of the BMA society (http://bma-society.org/).

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“…BM aspirates are obtained by aspiration of 5-10 mL from the iliac crest after infiltration of the area with local anesthetic (lidocaine, 10 mg/mL), in a 20 mL syringe and mixed 1:1 with basal media containing heparin (100 U/mL) or EDTA (1 mM) 1,2 .…”

Section: Isolation and Transportsmentioning

confidence: 99%

“…However, the methods used to isolate BMAT, BMAds and BMSCs, and the anatomical site(s) from which these are obtained, may influence their biological properties and the downstream analyses for which they can be used reliably. BMA is a young field that has recently received increasing attention among numerous scientific communities (1)(2)(3)(4)(5). The goal of this BMA biobanking position paper is to make protocols accessible for all interested researchers and to support researchers in designing their biobanks, sharing their samples and expertise to accelerate progress in BMA research.…”

Section: Introductionmentioning

confidence: 99%

Guidelines for Biobanking of Bone Marrow Adipose Tissue and Related Cell Types: Report of the Biobanking Working Group of the International Bone Marrow Adiposity Society

et al. 2021

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Over the last two decades, increased interest of scientists to study bone marrow adiposity (BMA) in relation to bone and adipose tissue physiology has expanded the number of publications using different sources of bone marrow adipose tissue (BMAT). However, each source of BMAT has its limitations in the number of downstream analyses for which it can be used. Based on this increased scientific demand, the International Bone Marrow Adiposity Society (BMAS) established a Biobanking Working Group to identify the challenges of biobanking for human BMA-related samples and to develop guidelines to advance establishment of biobanks for BMA research. BMA is a young, growing field with increased interest among many diverse scientific communities. These bring new perspectives and important biological questions on how to improve and build an international community with biobank databases that can be used and shared all over the world. However, to create internationally accessible biobanks, several practical and legislative issues must be addressed to create a general ethical protocol used in all institutes, to allow for exchange of biological material internationally. In this position paper, the BMAS Biobanking Working Group describes similarities and differences of patient information (PIF) and consent forms from different institutes and addresses a possibility to create uniform documents for BMA biobanking purposes. Further, based on discussion among Working Group members, we report an overview of the current isolation protocols for human bone marrow adipocytes (BMAds) and bone marrow stromal cells (BMSCs, formerly mesenchymal), highlighting the specific points crucial for effective isolation. Although we remain far from a unified BMAd isolation protocol and PIF, we have summarized all of these important aspects, which are needed to build a BMA biobank. In conclusion, we believe that harmonizing isolation protocols and PIF globally will help to build international collaborations and improve the quality and interpretation of BMA research outcomes.

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Bone Marrow Adipose Tissue Quantification by Imaging

Cited by 14 publications

References 97 publications

Intermittent fasting and continuous energy restriction result in similar changes in body composition and muscle strength when combined with a 12 week resistance training program

Intermittent fasting and continuous energy restriction result in similar changes in body composition and muscle strength when combined with a 12 week resistance training program

Report From the 6th International Meeting on Bone Marrow Adiposity (BMA2020)

Guidelines for Biobanking of Bone Marrow Adipose Tissue and Related Cell Types: Report of the Biobanking Working Group of the International Bone Marrow Adiposity Society

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