Bone Homeostasis in Experimental Fumonisins Intoxication of Rats

Abstract

fumonisins are strongly toxic metabolites of Fusarium proliferatum and Fusarium verticillioides commonly present in corn-based feed. the aim of the study was to evaluate bone homeostasis in experimental fumonisins B1 and B2 intoxication of rats, a vertebrate animal model of toxicological studies, as still little is known about the possible disturbing effect of fumonisins on bone homeostasis. adolescent (5-week-old) male Wistar rats were randomly assigned into a control group and a group fB intoxicated with fumonisins by daily intragastric administration of fumonisins at the dose of 90 mg/kg of body weight per animal in the fB group for 21 days. the fumonisin intoxication did not affect body and bone mass, although the mechanical and geometric properties were decreased in fumonisin-intoxicated rats. Bone volumetric and mineral density did not differ between groups, but bone mineral content and bone ash percentage was lower in the fB group. Detailed analysis showed that Ca, Cu, Fe, Mn, Sr, and Zn bone content significantly decreased in fumonisin intoxicated rats and the alterations in structure of bone mineral phase (reduction of the apatite-bone crystals size) were noted. While the negative structural alterations in growth plate and articular cartilages were also observed, fumonisin intoxication improved histomorphometrical parameters of trabecular bone. concluding, the dose of fumonisins used in the present study caused hepatotoxic effect, which was sufficient to trigger the disturbance in mineral homeostasis resulting in altered bone metabolism and decreased mechanical endurance.

The effect of bee pollen on bone biomechanical strength and trabecular bone histomorphometry in tibia of young Japanese quail (Coturnix japonica)

“…Bone geometry, weight or mineralization are not only indices for evaluating the morphological changes in bones after dietary treatment [42,43]. Also bone metaphysis microarchitecture can be an indicator of bone functionality, as bone longitudinal growth occurs as a consequence of conversion of cartilage to mineralized bone within the metaphysis [44,45]. Despite being 6 weeks old, our quails still seem to have developing bones, irrespective of line type, sex or bee pollen supplementation.…”

Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts?

“…In the current study, 2-Ox supplementation did not improve any of the indices of bone diaphysis geometry or bone breaking strength in the three-point bending test. However, bone material properties are better traits than raw bone breaking strength in measuring the effect of treatment on bone mechanical strength, as they can correct for bone size and testing procedure [22,25,40]. Higher values of ultimate stress, which is a measure of the internal resistance of a material to deformity, indicate that bones of gilts from the 2-Ox group were able to withstand higher stresses before fracture.…”

“…The measurement of bone mineral density (BMD) and bone mineral content (BMC) for the whole bone was performed using the dual-energy X-ray absorptiometry (DXA) method on a Discovery W densitometer (Hologic, Bedford, MA, USA), which was calibrated prior to the measurements using bone phantoms of known BMD [22].…”

“…Connective tissue homeostasis, which is regulated by many factors, varies in prenatal and postnatal life and can be affected by several hormones and nutritional factors [21][22][23][24][25]. The quality of food consumed by pregnant dams, or by their offspring during postnatal life, plays an important role in fetal growth and the subsequent development of newborns.…”

Effects of Yeast (Saccharomyces Cerevisiae) Probiotics Supplementation on Bone Quality Characteristics in Young Japanese Quail (Coturnix Japonica): The Role of Sex on the Action of the Gut-Bone Axis

“…Finally, the bones were defatted in a 1:1 mixture of chloroform and methanol for 24 h, weighed and ashed in a muffle furnace at 500 • C for 24 h to determine bone ash percentage. The percentage ash was determined relative to dry weight of the tibia [25].…”