Bone healing stimulated by plasma Factor XIII: Osteotomy experiments in sheep

Abstract

Sheep had their right metatarsals osteotomized midshaft and stabilized by plate and screws. One group was injected with 1250 units of Factor Thirteen for 9 days postoperatively, and a control group received placebo injections. After 8 weeks, the bones were evaluated biomechanically, histomorphologically and by densitometry. The bones of the treated group had a significantly higher tensile strength than the bones of the control animals. The correlation of biomechanical and morphological results demonstrated that the tensile strength increased with an increasing number of osteons crossing the osteotomy gap. The hydroxyapatite content of the bone healing zone was 7.3 per cent higher for the treated bones than for the control bones.

Normal Bone Deposition Occurs in Mice Deficient in Factor XIII-A and Transglutaminase 2

“…Further studies will determine whether transglutaminase activity is required for the repair of injured bone, in line with the general repair functions of transglutaminases and previous reports in the literature [48,50]. If FXIII-A plays a role in bone repair, this will not be mediated by FXIII-A-37, a protein that was misidentified with an antibody that, under certain circumstances, reacts preferentially with other antigens.…”

“…Various studies have used mAb AC-1A1 for cytochemical or histochemical detection of FXIII-A, for example in osteoblasts [11], megakaryocytes [20], rat heart [17] and human aortic valves [8,48]. Immunofluorescence labelling of tissue sections from wild-type and FXIII-A knockout mice indicates that mAb-AC-1A1 is capable of recognising intracellular FXIII-A when expressed at sufficient levels, although background staining is usually evident.…”

Structural properties of fracture haematoma: current status and future clinical implications

“…In spite of this, α – α crosslinking takes centre stage in determining the physical properties of clots, such as stiffness, elasticity and tautness, compared to γ – γ crosslinking (Duval et al ., ; Helms et al ., ). In clinical a setting, the local application of factor XIII into bone defects has been proposed in an effort to boost new bone formation, but the anticipated outcomes still remain controversial (Claes et al ., ; El‐Hakim, ; Hellerer et al ., ).…”

Tissue Transglutaminase and Factor XIII in Cartilage and Bone Remodeling

“…16 ' 17 Experiments conducted in ani mal models showed a stimulatory effect of factor XIII on bone healing, when applied locally and systemically. 18,19…”