Bone healing around titanium implants in two rat colitis models

Küchler, Uwe; Luvizuto, Eloá Rodrigues; Muñoz, Fernando; Hofbauer, Julia; Watzek, Georg; Gruber, Reinhard

doi:10.1111/j.1600-0501.2012.02454.x

Cited by 7 publications

(7 citation statements)

References 35 publications

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“…In agreement with previous studies based on a rat tibia implantation model, we observed peri‐implant bone formation of titanium screws in the cortical bone. In support of this claim, the medullary bone formation resembles one of previous studies; for example, BV/TV was around 20% (Kuchler et al., 2013 ), only slightly higher than the 10% in the present study. The cortical bone formation was approximately 90% in previous studies (Kuchler et al., 2011 , 2013 ), now also reaching 90% when combining new and old bone.…”

Section: Discussionsupporting

confidence: 92%

“…In support of this claim, the medullary bone formation resembles one of previous studies; for example, BV/TV was around 20% (Kuchler et al., 2013 ), only slightly higher than the 10% in the present study. The cortical bone formation was approximately 90% in previous studies (Kuchler et al., 2011 , 2013 ), now also reaching 90% when combining new and old bone. Under these conditions, BDL had not caused a significant and relevant change of the standard parameters of peri‐implant bone formation—the BV/TV and BIC.…”

Section: Discussionsupporting

confidence: 92%

“…First, the tibia model only moderately represents the situation of the jaw with its unique immunologic situation and the lack of functional loading of implants. Second, our study is a pilot as we have only selected one observation period based on previous studies with PTH (Kuchler et al., 2011 ; Mair et al., 2009 ), colitis (Kuchler et al., 2013 ), and vitamin D deficiency (Dvorak et al., 2012 ). Future studies should consider the long‐term effects of liver cirrhosis on osseointegration.…”

Section: Discussionmentioning

confidence: 99%

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Bone healing around titanium implants in a preclinical model of bile duct ligation‐induced liver injury

Talebian

Kampleitner

Sagl

et al. 2021

Clinical Oral Implants Res

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Objectives Chronic liver disease increases the risk for periodontal disease and osteoporotic fractures, but its impacts on bone regeneration remain unknown. Herein, we studied the impact of liver cirrhosis on peri‐implant bone formation. Material and Methods A total of 20 male Wistar rats were randomly divided into two groups: one with the common bile duct ligated (BDL) and the respective sham‐treated control group (SHAM). After four weeks of disease induction, titanium mini‐screws were inserted into the tibia. Successful induction of liver cirrhosis was confirmed by the presence of clinical symptoms. Another four weeks later, peri‐implant bone volume per tissue volume (BV/TV) and bone‐to‐implant contact (BIC) were determined by histomorphometric analysis. Results Peri‐implant bone formation was not significantly different between the SHAM and BDL groups. In the cortical compartment, the median percentage of peri‐implant new bone was 10.1% (95% CI of mean 4.0–35.7) and 22.5% (13.8–30.6) in the SHAM and BDL groups, respectively (p = .26). Consistently, the new bone in direct contact with the implant was 18.1% (0.4–37.8) and 23.3% (9.2–32.8) in SHAM and BDL groups, respectively (p = .38). When measuring the medullary compartment, the new bone area was 7.1% (4.8–10.4) and 10.4% (7.2–13.5) in the SHAM and BDL groups, respectively (p = .17). Medullary new bone in direct contact with the implant was 10.0% (1.2–50.4) and 20.6% (16.8–35.3) in SHAM and BDL groups, respectively, and thus comparable between the two groups (p = .46). Conclusions Bile duct ligation has no significant impact on the early stages of peri‐implant bone formation.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Bone healing around titanium implants in a preclinical model of bile duct ligation‐induced liver injury

Talebian

Kampleitner

Sagl

et al. 2021

Clinical Oral Implants Res

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“…After treating the images for the histomorphometric analysis, the bone area was quantified concerning the total tissue area of each sample using the same software (Cell Sens Dimensions, Olympus, Tokyo, Japan). The measurements made in this study for analysis were based on the study by Kuchler et al (2013) [ 24 ], as follows: Bone–implant contact (BIC): percentage of the implant surface in direct contact with the bone ( Figure 3 ). …”

Section: Methodsmentioning

confidence: 99%

Assessment of the Tissue Response to Modification of the Surface of Dental Implants with Carboxyethylphosphonic Acid and Basic Fibroblastic Growth Factor Immobilization (Fgf-2): An Experimental Study on Minipigs

Aragoneses

Suárez

López-Valverde

et al. 2021

Biology

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The aim of this study was to evaluate the effect of implant surface treatment with carboxyethylphosphonic acid and fibroblast growth factor 2 on the bone–implant interface during the osseointegration period in vivo using an animal model. The present research was carried out in six minipigs, in whose left tibia implants were inserted as follows: eight implants with a standard surface treatment, for the control group, and eight implants with a surface treatment of carboxyethylphosphonic acid and immobilization of FGF-2, for the test group. At 4 weeks after the insertion of the implants, the animals were sacrificed for the histomorphometric analysis of the samples. The means of the results for the implant–bone contact variable (BIC) were 46.39 ± 17.49% for the test group and 34.00 ± 9.92% for the control group; the difference was not statistically significant. For the corrected implant–bone contact variable (BICc), the mean value of the test group was 60.48 ± 18.11%, and that for the control group, 43.08 ± 10.77%; the difference was statistically significant (p-value = 0.035). The new bone formation (BV/TV) showed average results of 27.28 ± 3.88% for the test group and 26.63 ± 7.90% for the control group, meaning that the differences were not statistically significant (p-value = 0.839). Regarding the bone density at the interthread level (BAI/TA), the mean value of the test group was 32.27 ± 6.70%, and that of the control group was 32.91 ± 7.76%, with a p-value of 0.863, while for the peri-implant density (BAP/TA), the mean value of the test group was 44.96 ± 7.55%, and that for the control group was 44.80 ± 8.68%, without a significant difference between the groups. The current research only found a significant difference for the bone–implant contact at the cortical level; therefore, it could be considered that FGF-2 acts on the mineralization of bone tissue. The application of carboxyethylphosphonic acid on the surface of implants can be considered a promising alternative as a biomimetic coating for the immobilization of FGF-2. Despite no differences in the new bone formation around the implants or in the interthread or peri-implant bone density being detected, the biofunctionalization of the implant surface with FGF-2 accelerates the mineralization of the bone–implant interface at the cortical level, thereby reducing the osseointegration period.

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“…Research into bisphosphonates [ 347 , 355 , 357 , 358 , 359 ] or agonists like selective estrogen receptor modulators [ 347 , 360 ] is typically conducted in the rat as it is an excellent model of osteoporosis. Other diseases, such as arthritis [ 361 ] or Crohn’s disease [ 362 ] and the effects of severe dietary magnesium deficiency on systemic bone density, have been investigated [ 363 ]. Finally, the rat tibia can also be used for mechanical testing, including in dynamic loading models [ 364 , 365 ], as well as for exploring the effects of pulsed ultrasound [ 366 ] or laser therapy [ 367 ].…”

Section: Appendix A1 Research In Non-human Primatesmentioning

confidence: 99%

Pre-Clinical Models in Implant Dentistry: Past, Present, Future

et al. 2021

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Biomedical research seeks to generate experimental results for translation to clinical settings. In order to improve the transition from bench to bedside, researchers must draw justifiable conclusions based on data from an appropriate model. Animal testing, as a prerequisite to human clinical exposure, is performed in a range of species, from laboratory mice to larger animals (such as dogs or non-human primates). Minipigs appear to be the animal of choice for studying bone surgery around intraoral dental implants. Dog models, well-known in the field of dental implant research, tend now to be used for studies conducted under compromised oral conditions (biofilm). Regarding small animal models, research studies mostly use rodents, with interest in rabbit models declining. Mouse models remain a reference for genetic studies. On the other hand, over the last decade, scientific advances and government guidelines have led to the replacement, reduction, and refinement of the use of all animal models in dental implant research. In new development strategies, some in vivo experiments are being progressively replaced by in vitro or biomaterial approaches. In this review, we summarize the key information on the animal models currently available for dental implant research and highlight (i) the pros and cons of each type, (ii) new levels of decisional procedures regarding study objectives, and (iii) the outlook for animal research, discussing possible non-animal options.

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Bone healing around titanium implants in two rat colitis models

Abstract: The results suggest bone regeneration around implants is not impaired in chemically induced colitis models. Considering that Crohn's disease can affect any part of the gastrointestinal tract including the mouth, our model only partially reflects the clinical situation.

Cited by 7 publications

References 35 publications

Bone healing around titanium implants in a preclinical model of bile duct ligation‐induced liver injury

Bone healing around titanium implants in a preclinical model of bile duct ligation‐induced liver injury

Assessment of the Tissue Response to Modification of the Surface of Dental Implants with Carboxyethylphosphonic Acid and Basic Fibroblastic Growth Factor Immobilization (Fgf-2): An Experimental Study on Minipigs

Pre-Clinical Models in Implant Dentistry: Past, Present, Future

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