Bone Graft Prefabrication Following the In Vivo Bioreactor Principle

Huang, Ru‐Lin; Kobayashi, Eiji; Li, Kai; Li, Qingfeng

doi:10.1016/j.ebiom.2016.09.016

Cited by 56 publications

(50 citation statements)

References 113 publications

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“…Thus, the complete vascularization of large implants after transplantation needs a prolonged time period that is associated with major tissue loss due to hypoxic conditions. In plastic surgery, the flap prefabrication technique allows free tissue grafts to be transferred as pedicled flaps and has the major advantage that the tissue construct is directly reperfused after implantation at its final destination 2930. The success of the prefabricated buccal mucosa-lined flap for tubularized urethral reconstruction further confirmed that the flap prefabrication technique may have potential application for urethral tissue engineering.…”

Section: Discussionmentioning

confidence: 89%

Tubularized urethral reconstruction using a prevascularized capsular tissue prelaminated with buccal mucosa graft in a rabbit model

et al. 2019

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Tubularized graft urethroplasty fails largely because of inadequate graft take. Prefabrication of buccal mucosa lined flap has theoretical indications for constructing neourethra with an independent blood supply. The efficacy of using a tissue expander capsule as an induced vascular bed to prefabricate an axial vascularized buccal mucosa-lined flap for tubularized urethral reconstruction in a rabbit model was tested. The experiments were performed in three stages. First, silicone tissue expanders were inserted into the groin to induce vascularized capsule pouch formation. Next, buccal mucosa grafts were transplanted to the newly formed capsular tissue supplied by the axial vessel for buccal mucosa-lined flap prefabrication. Then, circumferential urethral defects were created and repaired by buccal mucosa graft (Group 1), capsule flap (Group 2) and prefabricated capsule buccal mucosa composite flap (Group 3). With retrograde urethrography, no rabbits in Group 1 maintained a wide urethral caliber. In Group 2, the discontinued epithelial layer regenerated at 1 month, and the constructed neourethra narrowed even though the lumen surface formed intact urothelial cells at 3 months. In Group 3, buccal mucosa formed the lining in the neourethra and kept a wide urethral caliber for 3 months. The capsule may serve as an induced vascular bed for buccal mucosa-lined flap prefabrication. The prefabricated buccal mucosa-lined flap may serve as a neourethra flap for circumferential urethral replacement.

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Section: Discussionmentioning

confidence: 89%

Tubularized urethral reconstruction using a prevascularized capsular tissue prelaminated with buccal mucosa graft in a rabbit model

et al. 2019

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“…Three factors play an important role in the engineering of cartilage tissue. Cells are somatic cells or stem cells [7]. Biomaterials are synthetic or natural materials.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, host cell ingrowth would impair the quality of reconstructed tissue. Therefore, a new trend to avoid the above problems is in vivo bioreactor (IV bioreactor), which can effectively prevent vascular invasion, host cell invasion and immune rejection [7]. This using the body as a bioreactor, the traditional three elements (scaffold, cell and growth factor) were cultured, and the regeneration environment of the body provided help for its growth.…”

Section: Introductionmentioning

confidence: 99%

In vivo bioreactor using cellulose membrane benefit engineering cartilage by improving the chondrogenesis and modulating the immune response

Park

Choi

et al. 2019

Preprint

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To regenerate tissue engineered cartilage as a source for the restoration of cartilage defects, we used a human fetal cartilage progenitor cell (hFCPC) pellet for improve the chondrogenesis and modulation the immune response with a In vivo (IV) bioreactor system, that was buried subcutaneously in the host and then implanted into a cartilage defect. In vivo bioreactor (IVB) was composed of silicone tube and cellulose nanopore-size membrane. FCPC pellets were first cultured in vitro for 3 days, and then cultured in vitro, subcutaneous and IV bioreactor for 3 weeks. First evaluated the IV bioreactor fluid appearance, component and liquidity, and then evaluate chondrogenesis and immunogenicity of the pellets using gross observation, cell viability, histology, biochemical analysis, RT-PCR, and Western Blot, finally evaluates the cartilage repair and synovial inflammation using histology. The fluid color and transparency of IV bioreactor were similar to synovial fluid (SF) and the component was also close to SF compared to the serum. IV bioreactor system not only promotes the synthesis of cartilage matrix and maintains cartilage phenotype, but also delays the occurrence of calcification compared with subcutaneous. A IV bioreactor, which has been predominantly adopted to study cell differentiation, was effective in preventing host immune rejection.

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“…It provides optimal conditions in terms of osseointegration and osteoconductivity in case of bone defect reconstruction, due to the living cells and the cellular matrix within the transplant leading to rapid bone regrowth [2].…”

Section: The Technical Challenge: Reconstruction Of Large-scale Tissumentioning

confidence: 99%

The Arteriovenous Loop: Engineering of Axially Vascularized Tissue

et al. 2018

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Background: Most of the current treatment options for large-scale tissue defects represent a serious burden for the patients, are often not satisfying, and can be associated with significant side effects. Although major achievements have already been made in the field of tissue engineering, the clinical translation in case of extensive tissue defects is only in its early stages. The main challenge and reason for the failure of most tissue engineering approaches is the missing vascularization within large-scale transplants. Summary: The arteriovenous (AV) loop model is an in vivo tissue engineering strategy for generating axially vascularized tissues using the own body as a bioreactor. A superficial artery and vein are anastomosed to create an AV loop. This AV loop is placed into an implantation chamber for prevascularization of the chamber inside, e.g., a scaffold, cells, and growth factors. Subsequently, the generated tissue can be transplanted with its vascular axis into the defect site and anastomosed to the local vasculature. Since the blood supply of the growing tissue is based on the AV loop, it will be immediately perfused with blood in the recipient site leading to optimal healing conditions even in the case of poorly vascularized defects. Using this tissue engineering approach, a multitude of different axially vascularized tissues could be generated, such as bone, skeletal or heart muscle, or lymphatic tissues. Upscaling from the small animal AV loop model into a preclinical large animal model could pave the way for the first successful attempt in clinical application. Key Messages: The AV loop model is a powerful tool for the generation of different axially vascularized replacement tissues. Due to minimal donor site morbidity and the possibility to generate patient-specific tissues variable in type and size, this in vivo tissue engineering approach can be considered as a promising alternative therapy to current treatment options of large-scale defects.

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Bone Graft Prefabrication Following the In Vivo Bioreactor Principle

Cited by 56 publications

References 113 publications

Tubularized urethral reconstruction using a prevascularized capsular tissue prelaminated with buccal mucosa graft in a rabbit model

Tubularized urethral reconstruction using a prevascularized capsular tissue prelaminated with buccal mucosa graft in a rabbit model

In vivo bioreactor using cellulose membrane benefit engineering cartilage by improving the chondrogenesis and modulating the immune response

The Arteriovenous Loop: Engineering of Axially Vascularized Tissue

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