Bone formation of demineralized human dentin block graft with different demineralization time: In vitro and in vivo study

Park, So-min; Kim, David-Hyungjin; Pang, Eun-Kyoung

doi:10.1016/j.jcms.2017.03.007

Cited by 21 publications

(15 citation statements)

References 30 publications

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“…Another study evaluated the characteristics of dentin blocks with different demineralization time (demineralized for 10 min, DDB10; for 60 min, DDB60), and assessed new bone formation after grafting the dentin blocks in rat calvaria defects. The DDB60 group resulted in significantly more new bone area than the DDB10 group at 8 weeks, but had higher resorption which reduced the total augmented area when compared with DDB10 [47]. The relationship between the demineralized degree and the new bone induction using DDM blocks was consistent with that using DDM particles [17].…”

Section: Ddm Preparationsupporting

confidence: 67%

“…Extensive studies have been conducted on DDM with its osteoinductivity and osteoconductivity in vitro and in vivo [17,18,[48][49][50][51]. In animal studies, DDM enhanced the bone healing process of tooth sockets, and promoted new bone formation in rat calvaria defects, rabbit parietal defects, minipig crania defects, sheep iliac defects, and porcine sinus [17,18,41,[47][48][49]52]. Besides bone, DDM was employed in osteochondral regeneration [20].…”

Section: Application Of Ddm In Bone Regenerationmentioning

confidence: 99%

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Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

et al. 2019

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Demineralized dentin matrix (DDM), derived from human teeth, is an excellent scaffold material with exciting bioactive properties to enhance bone and dental tissue engineering efficacy. In this article, first the nano-structure and bioactive components of the dentin matrix were reviewed. Then the preparation methods of DDM and the resulting properties were discussed. Next, the efficacy of DDM as a bone substitute with in vitro and in vivo properties were analyzed. In addition, the applications of DDM in tooth regeneration with promising results were described, and the drawbacks and future research needs were also discussed. With the extraction of growth factors from DDM and the nano-structural properties of DDM, previous studies also broadened the use of DDM as a bioactive carrier for growth factor delivery. In addition, due to its excellent physical and biological properties, DDM was also investigated for incorporation into other biomaterials design and fabrication, yielding great enhancements in hard tissue regeneration efficacy.

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Section: Ddm Preparationsupporting

confidence: 67%

Section: Application Of Ddm In Bone Regenerationmentioning

confidence: 99%

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

et al. 2019

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“…A comparison of 0.1 M EDTA and 0.6 M HCl revealed that EDTA resulted in an almost intact, hierarchically ordered collagen structure, while the hydrolysis induced by HCl destroyed the collagen structure 17 . Park et al 19 also investigated the HCl treatment time and showed that inorganic components and crystallinity sharply decreased as treatment time increased. However, a 60-min treatment time still could not eliminate all the mineral.…”

Section: Introductionmentioning

confidence: 99%

Comparison of different protocols for demineralization of cortical bone

Pang

Green

et al. 2021

Sci Rep

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Bone is a biological composite material consisting of two main components: collagen and mineral. Collagen is the most abundant protein in vertebrates, which makes it of high clinical and scientific interest. In this paper, we compare the composition and structure of cortical bone demineralized using several protocols: ethylene-diamine-tetraacetic acid (EDTA), formic acid (CH2O2), hydrochloric acid (HCl), and HCl/EDTA mixture. The efficiencies of these four agents were investigated by assessing the remaining mineral quantities and collagen integrity with various experimental techniques. Raman spectroscopy results show that the bone demineralized by the CH2O2 agent has highest collagen quality parameter. The HCl/EDTA mixture removes the most mineral, but it affects the collagen secondary structure as amide II bands are shifted as observed by Fourier transform infrared spectroscopy. Thermogravimetric analysis reveals that HCl and EDTA are most effective in removing the mineral with bulk measurements. In summary, we conclude that HCl best demineralizes bone, leaving the well-preserved collagen structure in the shortest time. These findings guide on the best demineralization protocol to obtain high-quality collagen from bone for clinical and scientific applications.

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“…Studies showed that demineralized dentin and bone had similar organic and inorganic structure. These studies reported that dentin could be used as a bone graft 22,23,24 . We used extracted human first premolar teeth due to orthodontic treatment and extracted rabbit teeth to obtain dentin graft materials.…”

Section: Discussionmentioning

confidence: 99%

“…Park et al used extracted human teeth for their research and demineralized the dentin with 0.6N HCl. Park et al compared the affect of different demineralization times of dentin on the new bone formation 24 . In another study, the researchers stored extracted bovine teeth at -80°C for 24 h and the teeth were demineralized in 0.6 N HCl for a week, then placed into chloroform methanol for 24 hours to obtain the dentin graft 29 .…”

Section: Discussionmentioning

confidence: 99%

Effects of dentin graft on bone mineral density of newly formed bone: an experimental animal study

Mercan

Sümer²,

Semirgen³

et al. 2018

Cukurova Medical Journal

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Öz Purpose: The aim of this study was to evaluate the bone mineral density of dentin used as bone graft material in bone defects. Materials and Methods: Sixteen male New Zealand white rabbits were used. Two critical-size calvarial bone defects were created in each rabbit and filled with different types of grafts. The bone defects were divided into four groups. The defects in group A1 were filled with processed dentin from human teeth; group A2 was filled with processed dentin from rabbit teeth; group B1 was filled with bovine bone (Bio-Oss), and the defects in group B2 were left empty. Bone mineral density was evaluated using dual-energy X-ray absorptiometry at the 4th and 12th weeks. Results: The bone mineral density values at the 4th week were not statistically different among groups A1, A2 and B1. However, the bone mineral density values at the 12th weeks were significantly higher for group B1 than the other groups. Histopathologic evaluations showed better bone-healing for group B1 than group A1 and A2. Conclusion: Dentin grafts and bovine bone graft have similar outcomes according to the bone mineral density values at the early term of bone-healing (4 weeks). However, in the late term of bone-healing (12 weeks), bone mineral density values of bovine bone were better than the other groups. Amaç: Bu çalışmanın amacı, kemik defektlerinde kemik grefti materyali olarak kullanılan dentinin kemik mineral yoğunluğunu değerlendirmektir. Gereç ve Yöntem: Çalışmada onaltı erkek Yeni Zelanda beyaz tavşanı kullanıldı. Her bir tavşanın kalvaryum kemiğinde ikişer kritik büyüklükte defekt oluşturuldu ve farklı greft materyalleri ile dolduruldu. Kemik defektleri dört gruba ayrıldı. A1 grubundaki defektler; insan dişlerinden elde edilmiş dentin ile; A2 grubundaki defektler; tavşan dişlerinden elde edilen dentin ile dolduruldu. B1 grubu sığır kemiğiyle (Bio-Oss) dolduruldu ve B2 grubundaki (kontrol) defektler boş bırakıldı. Kemik mineral yoğunluğu DEXA ile 4. ve 12. haftalarda değerlendirildi Bulgular: 4. haftada kemik mineral yoğunluğu değerleri A1, A2 ve B1 grupları arasında istatistiksel olarak anlamlı bulunmadı. Bununla birlikte, 12. haftadaki kemik mineral yoğunluğu değerleri grup B1 için diğer gruplara göre anlamlı derecede yüksek bulundu. Histopatolojik değerlendirmeye göre; B1 grubu, A1 ve A2 gruplarına kıyasla daha iyi bir kemik iyileşmesi gösterdi. Sonuç: Dentin greftleri ve sığır kemik grefti kemik iyileşmesinin erken dönemindeki (4. hafta) kemik mineral yoğunluğu değerlerine göre benzer sonuç sergilemiştir.. Bununla birlikte, kemik iyileşmesinin geç döneminde (12. hafta) sığır kemiğinin kemik mineral yoğunluğu değerleri diğer gruplardan daha iyi bulundu.

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Bone formation of demineralized human dentin block graft with different demineralization time: In vitro and in vivo study

Cited by 21 publications

References 30 publications

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

Comparison of different protocols for demineralization of cortical bone

Effects of dentin graft on bone mineral density of newly formed bone: an experimental animal study

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