Bone formation during forelimb regeneration: A microtomography (microCT) analysis

Stock, Stuart R.; Blackburn, David C.; Gradassi, Michael; Simon, Hans Georg

doi:10.1002/dvdy.10241

Cited by 40 publications

(27 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This suggests that up until 8 weeks post-amputation, the digits and the carpals/metacarpals elongate to contribute to the growth of the entire hand, and after this time, the palm stops growing but the digits continue to elongate the beginning of ossification in the advanced regenerate. This observation is supported by a recent study showing the beginnings of mineralization of the distal regenerate by day 37 post-amputation (Stock et al 2003). It should be noted that there is one previous study examining apoptosis in regenerating forelimbs of larval axolotls: Mescher et al (2000).…”

Section: Discussionsupporting

confidence: 73%

Growth and apoptosis during larval forelimb development and adult forelimb regeneration in the newt (Notophthalmus viridescens)

2004

View full text Add to dashboard Cite

Many of the genes involved in the initial development of the limb in higher vertebrates are also expressed during regeneration of the limb in urodeles such as Notophthalmus viridescens. These similarities have led researchers to conclude that the regeneration process is a recapitulation of development, and that patterning of the regenerate mimics pattern formation in development. However, the developing limb and the regenerating limb do not look similar. In developing urodele forelimbs, digits appear sequentially as outgrowths from the limb palette. In regeneration, all the digits appear at once. In this work, we address the issue of whether regeneration and development are similar by examining growth and apoptosis patterns. In contrast to higher vertebrates, forelimb development in the newt, N. viridescens, does not use interdigital apoptosis as the method of digit separation. During adult forelimb regeneration, apoptosis seems to play an important role in wound healing and again during cartilage to bone turnover in the advanced digits and radius/ulna. However, similar to forelimb development, demarcation of the digits in adult forelimb regeneration does not involve interdigital apoptosis. Outgrowth, rather than regression of the interdigital mesenchyme, leads to the individualization of forelimb digits in both newt development and regeneration.

show abstract

Section: Discussionsupporting

confidence: 73%

Growth and apoptosis during larval forelimb development and adult forelimb regeneration in the newt (Notophthalmus viridescens)

2004

View full text Add to dashboard Cite

show abstract

“…However, in contrast to this proximal-distal sequence, the portion of the humerus distal to the amputation site was found to fail to ossify in synchrony with the regenerating radius and ulna. This finding suggests that the replacement of cartilage with mineralized bone close to the amputation site is delayed with respect to other regenerating skeletal elements (Stock et al 2003).…”

Section: Bonementioning

confidence: 98%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

View full text Add to dashboard Cite

“…Microcomputed tomography, [1][2][3] or micro-CT, is a nondestructive X-ray imaging modality that has been commonly used to characterize natural bone [4][5][6][7] for almost 20 years. Although the use of micro-CT to image bone scaffolds for tissue engineering applications is less common, it has been used to characterize scaffold architecture, [8][9][10][11] in vitro scaffold degradation, 12,13 and bone growth into polymeric 14,15 and calcium phosphate 16,17 scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Micro‐CT based quantification of non‐mineralized tissue on cultured hydroxyapatite scaffolds

Hilldore

Wojtowicz

Johnson

2007

J Biomedical Materials Res

View full text Add to dashboard Cite

An improved method to determine material volumes from microcomputed tomography (micro-CT) data is presented. In particular, the method can account for materials with significantly overlapping peaks and small volumes. The example case is a hydroxyapatite scaffold cultured with osteoprogenitor cells. The histogram obtained from the micro-CT data is decomposed into a Gaussian attenuation distribution for each material in the sample, including scaffold, pore and surface tissue, and background. This is done by creating a training set of attenuation data to find initial parameters and then using a nonlinear curve fit, which produced R(2) values greater than 0.998. To determine the material volumes, the curves that simulated each material are integrated, allowing small volume fractions to be accurately quantified. Thresholds for visualizing the samples are chosen based on volume fractions of the Gaussian curves. Additionally, the use of dual-material regions helps accurately visualize tissue on the scaffold, which is otherwise difficult because of the large volume fraction of scaffold. Finally, the curve integration method is compared with Bayesian estimation and intersection thresholding methods. The pore tissue is not represented at all by the Bayesian estimation, and the intersection thresholding method is less accurate than the curve integration method.

show abstract

Bone formation during forelimb regeneration: A microtomography (microCT) analysis

Cited by 40 publications

References 34 publications

Growth and apoptosis during larval forelimb development and adult forelimb regeneration in the newt (Notophthalmus viridescens)

Growth and apoptosis during larval forelimb development and adult forelimb regeneration in the newt (Notophthalmus viridescens)

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Micro‐CT based quantification of non‐mineralized tissue on cultured hydroxyapatite scaffolds

Contact Info

Product

Resources

About