Bone defect healing enhanced by ultrasound stimulation: Anin vitro tissue culture model

Abstract

Ultrasound has many medical applications. Previous animal and clinical studies have clearly shown a positive effect of ultrasound on the rate of osseous repair. The present in vitro study was designed to elucidate the specific response of bony tissue to ultrasound treatment. Bilateral femora were obtained from 36 mature male Wistar rats. A bone defect was created at the center of each distal metaphysis. The femora were maintained for either 7 or 14 days in in vitro tissue culture and received 15 min of ultrasound stimulation or a sham exposure. The ultrasound intensity used was either 320 or 770 mW/cm2. Healing of the bone defect was evaluated by histomorphological examination and by analysis for the synthesis and secretion of prostaglandin E2. The results showed that ultrasound stimulation can accelerate both defect healing and trabecular bone regeneration. All experimental femoral defects treated with ultrasound healed faster than the untreated cortical defects, but only the defects receiving 770 mW/cm2 reached a level that was significantly different. The healing rate for the 320-mW/cm2 stimulated defects was intermediate between that of the 770-mW/cm2 and sham-exposed defects. With ultrasound stimulation, prostaglandin E2 secretion by the experimental femora decreased significantly. Changes in the prostaglandin synthesis and concentration were found to correspond to changes in the amount of trabecular regeneration and to acceleration of bone healing. This highly controlled and well-studied model of ultrasound stimulation of bone healing in vitro can be used to further examine the biological mechanisms involved.

Comparative Study of the Effects of Low-Level Laser and Low-Intensity Ultrasound Associated With Biosilicate® on the Process of Bone Repair in the Rat Tibia

“…Takikawa et al (12) observed in an experimental study that, after six weeks of US treatment, it had significantly increased the consolidation rate in fractures with bone nonunion, compared with the control group. The same results were found by Sun et al (14) and Lirani et al (15) .…”

Distraksiyon osteogenezinde düşük yoğunlukta ultrason stimülasyonunun kemik mineral yoğunluğuna etkisinin DEXA ile incelemesi

“…In vitro olarak yapılmış olan çalışmalarda, ultrason tedavisinin osteojenik hücreler üzerinde mekanik stres oluşturarak proliferasyonu ve osteoblastik aktiviteyi uyardığı gösterilmiştir. 28,35,37,38 Dyson ve Brookes 39 sıçanlar üzerinden uyguladıkları 500 mW/cm2'lik ultrason stimülasyonu ile bilateral fibular fraktürlerini incelemişler; ultrasonun özellikle fraktürün erken safhalarında etkili olduğu bulgusuna ulaşmışlardır. Ding ve ark.…”

Η Επίδραση Της Χορήγησης Αντιβιοτικών Στην Πώρωση Των Καταγμάτων

“…Μεταξύ αυτών αναφέρονται η εφαρμογή ηλεκτρικών ερεθισμάτων, ηλεκτρομαγνητικών πεδίων, υπερήχων, 128,129 το υπερβαρικό οξυγόνο και η χρήση οστικών αλλομοσχευμάτων. 130,131,132,133,44,134,135 Είναι σαφές, ότι είναι δύσκολος ο έλεγχος της θετικής ή της αρνητικής επίδρασης στην πώρωση κατά την εξέλιξή της.…”