Bone/cartilage organoid on-chip: Construction strategy and application

Yan, Hu; Zhang, Hao; Wang, Sicheng; Cao, Liehu; Zhou, Fengjin; Jing, Yingying; Su, Jiacan

doi:10.1016/j.bioactmat.2023.01.016

Cited by 19 publications

(16 citation statements)

References 163 publications

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“…There is evidence that changes to the subchondral bone contribute to OA onset and progression [3,13]. Moreover, abnormal subchondral bone remodeling is essential for OA pathology [20][21][22][23]. Our results showed that abnormal bone remodeling of the tibia was observed in the DMM group.…”

Section: Discussionsupporting

confidence: 48%

The Therapeutic Role of Hyperoside in Medial Meniscus Destabilization-Induced Osteoarthritis: A Study on Subchondral Bone Remodeling

Yang,

Chen,

Tang

et al. 2024

Preprint

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Background The process of subchondral bone remodelling plays an imperative role in the progressive development of OA. Hyperoside (Hyp) is a flavonoid, which has a wide range of pharmacological effects. This study aims to explore the effect of Hyp on the subchondral bone to elucidate the therapeutic role of Hyp in medial meniscus destabilization (DMM) induced OA.Methods Firstly, we conducted histological research(HE staining, Safranin-O/Fast Green and Toluidine blue staining, TRAP staining, IHC) and microCT to test the ability of Hyp on cartilage degeneration and abnormal subchondral microstructural changes in the DMM-induced osteoarthritic mouse model. Secondly, In the in vitro experiments, RAW264.7 cells were induced to osteoblasts in the presence of different concentrations of Hyp and osteoclasts were labelled by TRAP staining. MC3TC-E1 cells were used to perform osteoinduction experiments in Osteogenic Induction Media (OIM). Osteogenic activity was observed through Alizarin red S staining, and mineralisation activity was observed through ALP staining. Last, Finally, the effect of Hyp on NF-κB pathways was studied using Western blot and immunofluorescence.Results Hyp decreased cartilage degeneration and improved BV/TV and Tb.Th structural parameters. It also reduced the number of TRAP-positive osteoclasts, nestin cells, and osterix cells in the subchondral bone. Additionally, Hyp Inhibits osteoclast formation and enhanced alkaline phosphatase activity and mineralization. Furthermore, the NF-κB signalling pathway related to osteoclasts was inhibited.Conclusion These results indicate that Hyp has potential therapeutic value for OA by modulating osteoclastogenesis and osteoblastogenesis in the subchondral bone.

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Section: Discussionsupporting

confidence: 48%

The Therapeutic Role of Hyperoside in Medial Meniscus Destabilization-Induced Osteoarthritis: A Study on Subchondral Bone Remodeling

Yang,

Chen,

Tang

et al. 2024

Preprint

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“…Since the BMSCs bank from the aging osteoporotic patients demonstrated the multipotent differentiation capability, the interaction of BMSCs with the microenvironment can be further explored to interpret the biological function of BMSCs in the osteoporotic tissue. In addition, bio-macromolecular signals, such as the exosome, microRNA, and interleukin released from the OP BMSCs, can be examined to delineate the mutual interaction between OP BMSCs and the bone mass microenvironment (29).…”

Section: Discussionmentioning

confidence: 99%

Persistent mesodermal differentiation capability of bone marrow MSCs isolated from aging patients with low energy traumatic hip fracture and osteoporosis: clinical evidence

Wang

Ding

et al. 2023

Preprint

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Background Low-energy trauma-elicited bone fractures represent a sign of bone fragility and ongoing osteoporosis. The low energy hit, such as a slight fall down from a bed, standing height, or fewer, results in a bone fracture, especially in the hip, which is a life-threatening risk for the older adult and a heavy burden for the social economics. Bone can self-heal and maintain the homeostasis of anabolism and catabolism to preserve a healthy bony structure. Meanwhile, bone marrow-derived stem cells (BMSCs) are critical in osteogenesis, leading to metabolic homeostasis in the healthy bony microenvironment. Notably, patients with low-energy traumatic bone fractures usually suffer a higher level of bony catabolism accompanied by osteoporosis. However, whether the BMSCs derived from the patients who suffered osteoporosis and low-energy traumatic hip fracture preserve a sustained mesodermal differentiation capability, especially in osteogenesis, is yet to be explored in a clinical setting.Methods Therefore, we collected BMSCs from clinical hip fracture patients accompanied by evidenced osteoporosis in the current study. The CD markers identification, cytokines examination, and osteogenic and adipogenic differentiation were evaluated and compared with the BMSCs withdrawn from the healthy young donors. Meanwhile, the BMSCs doubling time, doubling level, and cumulative population doubling level of proliferation test were also examined.Results Data reveals that BMSCs collected from elderly osteoporotic patients expressed more abundant interleukin 6 (IL-6) and vascular endothelial growth factor (VEGF) than the BMSCs collected from young donors. However, the CD markers and osteogenic and adipogenic differentiation capability in these elderly osteoporotic patients and healthy young donors are identical. Notably, the proliferation capability of BMSCs derived from aging osteoporotic patients compared with BMSCs from healthy juvenile donors reveal an equivalent level in passages 3 ~ 4 that is conventionally used for clinical cell transplantation.Conclusion Collectively, our data evidence that BMSCs derived from elderly osteoporotic patients hold the equivalent differentiation capability identical to BMSCs derived from youth, and the unbalanced bony homeostasis and subsequent osteoporosis in the older adult need further detailed exploration.

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“…Bone cells, including osteoblasts, osteocytes, and osteoclasts, contribute to the continuous remodeling process that maintains bone integrity. However, traumatic injuries, degenerative diseases, and congenital abnormalities may lead to bone defects, necessitating innovative strategies for bone tissue regeneration and repair [128]. In recent years, tissue engineering has emerged as a promising approach for constructing bone-like organoids that closely mimic native bone structures and functions [129].…”

Section: Bonementioning

confidence: 99%

Organoid bioinks: construction and application

Wang,

Song,

Wang

et al. 2024

Biofabrication

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Organoids have emerged as crucial platforms in tissue engineering and regenerative medicine but confront challenges in faithfully mimicking native tissue structures and functions. Bioprinting technologies offer a significant advancement, especially when combined with organoid bioinks-engineered formulations designed to encapsulate both the architectural and functional elements of specific tissues. This review provides a rigorous, focused examination of the evolution and impact of organoid bioprinting. It emphasizes the role of organoid bioinks that integrate key cellular components and microenvironmental cues to more accurately replicate native tissue complexity. Furthermore, this review anticipates a transformative landscape invigorated by the integration of artificial intelligence with bioprinting techniques. Such fusion promises to refine organoid bioink formulations and optimize bioprinting parameters, thus catalyzing unprecedented advancements in regenerative medicine. In summary, this review accentuates the pivotal role and transformative potential of organoid bioinks and bioprinting in advancing regenerative therapies, deepening our understanding of organ development, and clarifying disease mechanisms.

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Bone/cartilage organoid on-chip: Construction strategy and application

Cited by 19 publications

References 163 publications

The Therapeutic Role of Hyperoside in Medial Meniscus Destabilization-Induced Osteoarthritis: A Study on Subchondral Bone Remodeling

The Therapeutic Role of Hyperoside in Medial Meniscus Destabilization-Induced Osteoarthritis: A Study on Subchondral Bone Remodeling

Persistent mesodermal differentiation capability of bone marrow MSCs isolated from aging patients with low energy traumatic hip fracture and osteoporosis: clinical evidence

Organoid bioinks: construction and application

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