Bone atrophy by disuse in adult rats

Sevastik, J. A.; Larsson, Sven‐Erik; Mattson, Sören

doi:10.1007/bf02065279

Cited by 3 publications

(1 citation statement)

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“…The importance of the mechanical environment for developing musculoskeletal tissues cannot be understated, as mechanical forces are known to influence these tissues' performance. For example, numerous experiments on disuse of musculoskeletal tissues like cartilage, muscle, and bone have been correlated to apoptosis within the tissues, followed by their subsequent atrophy 8–10. Additionally, in vitro studies with meniscus explants and cells have shown that mechanical stimuli such as direct compression, tension, and HP can increase extracellular matrix (ECM) expression and synthesis 4, 11–18.…”

Section: Introductionmentioning

confidence: 99%

Effects of hydrostatic pressure on leporine meniscus cell‐seeded PLLA scaffolds

Gunja

Athanasiou

2009

J Biomedical Materials Res

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Hydrostatic pressure (HP) is an important component of the loading environment of the knee joint. Studies with articular chondrocytes and TMJ disc fibrochondrocytes have identified certain benefits of HP for tissue engineering purposes. However, similar studies with meniscus cells are lacking. Thus, in this experiment, the effects of applying 10 MPa of HP at three different frequencies (0, 0.1, and 1 Hz) to leporine meniscus cell-seeded PLLA scaffolds were examined. HP was applied once every 3 days for 1 h for a period of 28 days. Constructs were analyzed for cellular, biochemical, and biomechanical properties. At t = 4 weeks, total collagen/scaffold was found to be significantly higher in the 10 MPa, 0 Hz group when compared with other groups. This despite the fact that the cell numbers/scaffold were found to be lower in all HP groups when compared with the culture control. Additionally, the total GAG/scaffold, instantaneous modulus, and relaxation modulus were significantly increased in the 10 MPa, 0 Hz group when compared with the culture control. In summary, this experiment provides evidence for the benefit of a 10 MPa, 0 Hz stimulus, on both biochemical and biomechanical aspects, for the purposes of meniscus tissue engineering using PLLA scaffolds.

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Section: Introductionmentioning

confidence: 99%