Bone and Cartilage from Stem Cells: Growth Optimalization and Stabilization of Cell Phenotypes

Mimic miRNA and Anti-miRNA Activated Scaffolds as a Therapeutic Strategy to Promote Bone, Cartilage, and Skin Regeneration

“…MSCs have been identified as an attractive cell source for cartilage regeneration due to their ease of isolation, regenerative potential, and chondrogenic differentiation [162].…”

Regenerative Medicine and Tissue Engineering

“…(A) JUN = transcription factor AP-1, known to be mandatory for osteoblast differentiation , appears to exert a major impact on mir-339, and mir-149 in a hierarchical feed-forward fashion. (B) ETS1 and SP3, two of the 14 TFs constituting the selection criteria for the osteochondral microRNA profile (Gordeladze, Reseland et al, 2011), appeared to serve as inductors of several microRNAs, among which we find the microRNA species mir-149, mir-328, and mir-339. However, ETS1 and SP3 (along with SP1 and SP7 = Osterix) are up-regulated in osteoblastic cells.…”

“…Especially mir-149 seems to serve as a switch, both stimulating chondrogenesis, while suppressing osteoblastogenesis in differentiating hMSCs. Furthermore, mir-328 and mir-339 are the better determinants of the osteochondral cell phenotypes, since manipulations of their intracellular levels enabled us to reciprocally trans-differentiate osteoblasts to chondrocytes at day 7-14 in 2D and 3D cultures in vitro Gordeladze 2011). Hence, we propose that these six microRNAs, and mir-149, mir-328, and mir-339 in particular, might be able to influence the osteochondral phenotypes in such a way that they are able to maintain their phenotype in vitro and in vivo for an extended period of time, while under the influence of a Th-cell invaded arthritic luminal space.…”

“…At the end of the experiment, the number of TRAP-positive cells was counted, and the eroded dentine surface (%) was estimated in a reflecting light microscope. B. Co-culture of chondrocytes in alginate beads (or osteoblasts in 2D culture) in standard medium (Gordeladze 2011). The subject experimental setting was also applied to differentiating osteoblast.…”

“…The subject experimental setting was also applied to differentiating osteoblast. Here, we used the cytokine mixture as described in (B), and antago-microRNAs counteracting the os- (Gordeladze 2011). The above mentioned experimental setting was used with chondrocytes differentiated from hMSCs in alginate beads in the absence or presence of cytokines (IL-1, IL-6, IL-17, and TNFα), pre-microRNAs of all microRNA species of the osteochondral signature, or the set of cytokines along with the pre-microRNAs.…”