Bond strength of adhesive restorations to Er:YAG laser-treated
dentin

Malta, Daniel Alexandre Menezes Pedrosa; Kreidler, M. A. M.; Villa, Girlene E.P; Andrade, Marcelo Ferrarezi de; Fontana, Carla Raquel; Lizarelli, R. F. Z.

doi:10.1002/lapl.200610085

Cited by 42 publications

(24 citation statements)

References 25 publications

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“…This phenomenon could explain findings from Malta et al [13] when dentin treatment using Er:YAG laser with low fluencies did not affect glass ionomer cement adhesion, different as occurs to dental adhesive system based in composite resin.…”

Section: Discussionmentioning

confidence: 73%

Caries resistance of lased human enamel with Er:YAG laser – morphological and ratio Ca/P analysis

et al. 2006

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Abstract:Objective: "In vitro" analysis of caries resistance of dental enamel under caries simulation after irradiation with Er:YAG laser. Background Data: More susceptible to caries development spots at adjacent hard tissues from cavity preparations of dental tissues using burrs or lasers are quite common. Methods: Thirteen caries-free third permanent human molars were distributed as follows: G1: sound control and caries control; G2: Er:YAG 100, 200, 300, or 400 mJ/ 10 Hz/ 3 sec.; G3: the same parameters of G2 followed by artificial caries simulation, through dynamic model of demineralization and remineralization (DE/RE). Caries resistance analysis was evaluated through scanning electron microscopy (SEM) and Ca/P rate (X-Rays spectroscopy -EDX). Results: Photomicrographs showed that the Er:YAG laser created craters with rough aspect which became more evident as the energy per pulse was increased, but without change of regular morphology of enamel prisms. Significant statistical changes among the irradiated and control groups was observed considering the Ca/P ratio. Conclusion: Irradiated groups showed higher caries resistance than control groups. However, it is not possible to affirm that the enamel surface accidental irradiation could be a benefit to caries resistance for other situations can be considered, as biofilm deposit, which could increase the caries susceptibility.Image from G1 group (control + caries) under 1000× magnification

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Section: Discussionmentioning

confidence: 73%

Caries resistance of lased human enamel with Er:YAG laser – morphological and ratio Ca/P analysis

et al. 2006

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“…The animals were separated in three groups: the control group (GI -without laser irradiation) and the other two experimental groups, both of them using laser irradiation changing fluencies: GII -12.8 J/cm 2 (10 mW of power, time irradiation of 10 seconds and intensity of 12.8 × 10 2 mW/cm 2 ); and, GIII -89.7 J/cm 2 (70 mW of power, during 10 seconds of irradiation time and intensity of 89.7 × 10 2 mW/cm 2 . Histological studies were conducted using optic microscopy and software KS 400, version 2.0 Germany, Karl Zeiss at four periods of time (8,14,28, and 52 days), determined after cavity preparation. The swine were sacrificed and the teeth were cut from the maxilla along with part of the bone, stored in formaldehyde at 4% and sent for histological analysis.…”

Section: Methodsmentioning

confidence: 99%

In vivo analysis of low-power laser effects irradiation at stimulation of reactive dentine

et al. 2007

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Investigate and compare the amount of reactive dentine formation after application of two different flunkies of low level laser. Rapid formation of reparative dentine is convenient, mainly when pulp is almost exposed. Several studies have shown that the use of correct fluency of low level laser can enhance cellular proliferation and seems to stimulate the production of reactive dentine. Using 780 nm infrared laser, twelve swine were tried using 3 teeth each. Three treatments groups were classified: GI -control group: without irradiation; GII -irradiation using fluency of 12.8 J/cm 2 ; and, GIII -irradiation using fluency of 89.7 J/cm 2 . Laser irradiation was performed on dentine (class V standardized) of the upper and lower incisors and on the apex of each tooth for 3 consecutive times, with a 72 h break of time. Histological studies were conducted at 4 distinct periods of time (8, 14, 28, and 52 days). GII showed increase on the formation of reparative dentine with significant difference (p < 0.01) between GI and GIII. Comparison between GI and GIII shows that there was no significant difference at 4 periods of time. After 52 days, the amount of reparative dentine was similar between GI, GII, and GIII. The dentinal matrix formation equal primary dentine occurred earlier in the GIII, GII, and GI, respectively. Our results suggest that into a twenty eight days period, the 12.8 J/cm 2 laser group was able to positively stimulate it, accelerating the odontoblast process and after 52 days, the formation of reactive dentine seems stabilized. C OLHistological results from GIII, considering fourteen days of period of time to evaluation

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“…Low level laser (LLL) has been extensively investigated in the search for ideal conditions of cell biomodulation [1][2][3][4][5]. The LLL interacts with different types of tissues and LLL therapy (LLLT) is known to cause varied reactions and modulate several biological processes.…”

Section: Introductionmentioning

confidence: 99%

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Tagliani¹,

Oliveira²,

Lins³

et al. 2010

Laser Phys. Lett.

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In spite of knowing that cells under stress are biostimulated by low level laser (LLL) irradiation, the ideal condition of stress to different cell lines has not yet been established. Consequently, the aim of the present in vitro study was to evaluate the effects of a defined parameter of LLL irradiation applied on stressed odontoblast-like pulp cells (MDPC-23). The cells were seeded (12500 cells/cm 2 ) in wells of 24-well plates using complete culture medium (DMEM) and incubated for 24 hours. Then, the DMEM was replaced by a new medium with low concentrations (nutritional stress condition) of fetal bovine serum (FBS) giving rise to the following experimental groups: G1: 2% FBS; G2: 5% FBS; and G3: 10% FBS. The cells were irradiated three times with LLL in specific parameters (808±3 nm, 100 mW, 1.5 J/cm 2 ) every 24 hours. No irradiation was carried out in groups G4 (2% FBS-Control), G5 (5% FBS-Control), and G6 (10% FBS-Control). For all groups, the cell metabolism (MTT assay) and morphology (SEM) was evaluated. The experimental groups showed enhanced cell metabolism and normal cell morphology regardless of FBS concentration. A slight increase in the cell metabolism was observed only in group G2. It was concluded that cell nutritional stress caused by reducing the concentration of FBS to 5% is the most suitable method to assess the biostimulation of LLL irradiated MDPC-23 cells.MDPC-23 cells adhered to the glass substrate exhibited a spindle-shaped morphology and some mitosis were observed (arrows); SEM ×500

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Bond strength of adhesive restorations to Er:YAG laser-treated dentin

Cited by 42 publications

References 25 publications

Caries resistance of lased human enamel with Er:YAG laser – morphological and ratio Ca/P analysis

Caries resistance of lased human enamel with Er:YAG laser – morphological and ratio Ca/P analysis

In vivo analysis of low-power laser effects irradiation at stimulation of reactive dentine

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

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