Bombyxin-stimulated ecdysteroidogenesis in relation to sugar transporter/trehalase expressions in Bombyx prothoracic glands

Gu, Shi‐Hong; Chang, Chia-Hao; Lin, Pei‐Ling

doi:10.1016/j.ibmb.2022.103864

Cited by 4 publications

(3 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous study demonstrated that bombyxin treatment significantly increased St4 gene expression levels in silkworm prothoracic glands (Gu et al, 2022). We further investigated temporal changes in St4 gene expression levels in diapause and HCl‐treated eggs.…”

Section: Resultsmentioning

confidence: 96%

“…Thus, we suggest that increased St4 and St3 gene expression levels during early embryonic development may also be related to morphological changes in the embryo. St4 and St1 are downstream target genes involved in bombyxin‐stimulated prothoracic glands during the larval stage (Gu et al, 2022). A previous study demonstrated that higher bombyxin signaling was detected in HCl‐treated eggs compared to diapause eggs (Gu et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Ecdysteroids in dechorionated eggs and medium were extracted with methanol and measured using a 20‐hydroxyecdysone (20E) EIA kit (Cayman Chemical/Sanbio) as previously described (Gu et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Expressions of sugar transporter genes during Bombyx mori embryonic development

Lin

Chang

2023

J Exp Zool Pt A

View full text Add to dashboard Cite

Sugar transporters (Sts) play important roles in controlling carbohydrate transport and are responsible for mediating the movement of sugars into cells. Few studies have been conducted on expressions of Sts during insect embryonic development. In the present study, we investigated temporal expressions of St genes during the embryonic diapause process in Bombyx mori. We found that in HCl‐treated developing eggs, high gene expressions of trehalose transporter 1 (Tret1) were detected during middle and later embryonic development. St4 and St3 gene expressions gradually increased during the early stages, reached a small peak on Day 3, and large peaks were again detected on Day 7. However, in diapause eggs, expression levels of the Tret1, St4, and St3 genes all remained at low levels. Differential temporal changes in expressions of the Tret1, St4, and St3 genes found between diapause and HCl‐treated eggs were further confirmed using nondiapause eggs. Our results showed that nondiapause eggs exhibited similar changing patterns as those of HCl‐treated eggs, thus clearly indicating potential correlations between expressions of these genes and embryonic development. In addition, high gene expressions of Tret1 were also detected when dechorionated eggs were incubated in the medium. The addition of LY294002 (a specific phosphatidylinositol 3‐kinase [PI3K] inhibitor) and U0126 (a mitogen‐activated protein kinase/extracellular signal‐regulated kinase [ERK] kinase [MEK] inhibitor) partially inhibited Tret1 gene expression in dechorionated eggs, but did not affect either ecdysteroid‐phosphate phosphatase gene expression or ecdysteroid biosynthesis, clearly indicating that both PI3K and ERK are involved in increased gene expression of Tret1 that was independent of ecdysteroid levels. To our knowledge, this is the first comprehensive report to demonstrate the transcriptional regulation of St genes during embryonic development, thus providing useful information for a clearer understanding of insect egg diapause mechanisms.

show abstract

Section: Resultsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%