Bombyx mori transferrin: Genomic structure, expression and antimicrobial activity of recombinant protein

Yun, Eun‐Young; Lee, Jae-Keum; Kwon, O‐Yu; Hwang, Jae‐Sam; Kim, Iksoo; Kang, Seok-Woo; Lee, Won–Jae; Ding, Jeak Ling; You, Kwan‐Hee; Goo, Tae-Won

doi:10.1016/j.dci.2009.05.008

Cited by 42 publications

(38 citation statements)

References 27 publications

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“…In contrast, an analysis of Tsf1 sequences indicates a broad range in the number introns in the ORF (Tables 1a and 1b; [1,19,37,70]). Aedes aegypti (AaTsf1; yellow fever mosquito; [19,29]) has only 1 intron, while the gene for Bombyx mori Tsf1 (BmTsf1; silk worm) is relatively long and structurally more similar to human Tsf with approximately 12 introns [70]. Generally, Tsf1 genes from insects of the order Diptera including, A. aegypti, Culex quinquefasciatus (Southern house mosquito, West Nile virus vector) and Drosophila melanogaster (fruit fly) have half the number of ORF introns found in mammalian Tsf genes or fewer [1,19,37,71].…”

Section: Features Of Genomic and Primary Structurementioning

confidence: 88%

“…Mammalian Tsf genes are characterized by many introns in the open reading frame (ORF); Tsf, LF and OTsf each have 16 [1,19]. In contrast, an analysis of Tsf1 sequences indicates a broad range in the number introns in the ORF (Tables 1a and 1b; [1,19,37,70]). Aedes aegypti (AaTsf1; yellow fever mosquito; [19,29]) has only 1 intron, while the gene for Bombyx mori Tsf1 (BmTsf1; silk worm) is relatively long and structurally more similar to human Tsf with approximately 12 introns [70].…”

Section: Features Of Genomic and Primary Structurementioning

confidence: 92%

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Insect transferrins: Multifunctional proteins

Geiser

Winzerling

2012

Biochimica et Biophysica Acta (BBA) - General Subjects

115

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Section: Features Of Genomic and Primary Structurementioning

confidence: 88%

Section: Features Of Genomic and Primary Structurementioning

confidence: 92%

Insect transferrins: Multifunctional proteins

Geiser

Winzerling

2012

Biochimica et Biophysica Acta (BBA) - General Subjects

115

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“…The silkworm can also express high levels of foreign genes through the Baculovirus system. These resultant recombinant proteins may be used as vaccine antigens (3) and antibacterial proteins (4), such as the Cholera toxin B subunit (CTB) and Transferrin (Tf), respectively. In 2004, Mita et al completed genomic sequencing of the entire 500 Mb B. mori genome (5) and over 11,000 of the expressed sequence tags (EST) were analyzed (6,7).…”

Section: Introductionmentioning

confidence: 99%

Tissue-specific gene expression analysis of silkworm (Bombyx mori) by quantitative real-time RT-PCR

Park

Kang²,

Goo³

et al. 2010

BMB Reports

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The Bombyx mori Microarray Database (BmMDB; http://silkworm.swu.edu.cn/microarray) provides information for tissue-specific gene expression by using the whole-genome oligonucleotide microarray in the silkworm. We analyzed the tissue-specific expression patterns in the silk gland, fat body, and midgut five days of fifth instar larvae during the development of B. mori. To verify the tissue-specific expression, analysis was conducted using quantitative Real-time RT-PCR and the highly expressed endogenous Actin RNA as an intrinsic reference. Finally, we confirmed five genes, (sw15872, sw00692, sw20990, sw05300,and sw2250), out of 18 candidates expressed in two different tissues, which was consistent with the data published by Dr. Xiang's group, thereby supporting the BmMDB. Further studies for promoter regions of candidate genes can be applied in creating transgenic silkworms as biomedical insects for use in producing biomaterials, and to serve as well-characterized models for understanding the mechanism for the genetic regulation of tissue-specific development.[BMB reports 2010; 43(7): 480-484]

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“…Transcription and translation of the transferrin protein are upregulated by bacterial infection in several species of insects, including Lepidoptera (Nichol et al, 2002;Seitz et al, 2003;Ampasala et al, 2004;Yun et al, 2009;Guz et al, 2012). Here we demonstrate that SpliTrf is also upregulated in response to challenge with a gram-positive bacterium, B. thuringiensis.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it is known that transferrin synthesis is increased following exposure to bacteria, fungi, pathogens, and parasites, as well as insect parasitoids (Beernsten et al, 1994;Yoshiga et al, 1997Yoshiga et al, , 1999Kucharski et al, 2003;Thompson et al, 2003;Ampasala et al, 2004;Valles et al, 2005;Bergin et al, 2006;Lee et al, 2006;Guz et al, 2007Guz et al, , 2012Magalhaes et al, 2007;Paily et al, 2007;Wang et al, 2007;Mowlds et al, 2008;Yun et al, 2009;Zhou et al, 2009;Kim et al, 2010). Interestingly, although transferrin expression was found to be induced by dengue virus infection of the salivary glands in yellow fever mosquito, Aedes aegypti (Luplertlop et al, 2011), transferrin protein levels were shown to be downregulated (Tchankouo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis

Güz¹,

Dageri²,

Erdoğan³

et al. 2013

Turk J Biol

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Insect immune responses involved in virus defense have lately received increasing attention. While the pathways and effector molecules active in defense against bacteria and fungi are well studied (Ferrandon et al., 2007;Levitin et al., 2008), the regulation of the innate immune system against viral infections still remains to be elucidated. The baculoviruses (Baculoviridae) are doublestranded DNA viruses that infect arthropods, mainly insects and in particular Lepidoptera (Cory and Myers, 2003). Baculoviruses are not only biocontrol agents of lepidopteran pests, but also have been developed and used extensively in research, serving as expression vectors for high-level production of recombinant proteins (Bonning and Hammock, 1996;Susurluk et al., 2013). Although baculoviruses have been successfully used to control lepidopteran and hymenopteran insect pests of agriculture and forestry importance worldwide, little is known about the host immune responses towards these viral infections (Federici, 1986;Miller, 1997; Moscardi, 1999).Transferrin is an iron-binding protein that has a role in iron transport (Nichol et al., 2002), in preventing oxidative stress, and in delivering iron to eggs for development (Yoshiga et al., 1997). In addition, it is known that transferrin synthesis is increased following exposure to bacteria, fungi, pathogens, and parasites, as well as insect parasitoids (

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Bombyx mori transferrin: Genomic structure, expression and antimicrobial activity of recombinant protein

Cited by 42 publications

References 27 publications

Insect transferrins: Multifunctional proteins

Insect transferrins: Multifunctional proteins

Tissue-specific gene expression analysis of silkworm (Bombyx mori) by quantitative real-time RT-PCR

Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis

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