Bombyx mori prohemocyte division and differentiation in individual microcultures

Yamashita, Miyuki; Iwabuchi, Kikuo

doi:10.1016/s0022-1910(00)00144-x

Cited by 87 publications

(74 citation statements)

References 21 publications

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“…So far, the effects of hormones on each cell type have not been studied, since all studies to date have been performed with mixed hemocyte populations. Yamashita and Iwabuchi (2001) recently showed that single-cell culture can provide much information about the fate of individual B. mori hemocytes in vitro. Preliminary studies on the effect of insect hormones on B. mori hemocytes using the single-cell culture technique suggested that the mitotic division of granulocytes was stimulated by bombyxin-II.…”

Section: Introductionmentioning

confidence: 99%

“…Granulocytes predominate B. mori larval hemolymph (66.8% in 4th instar larvae). These cells are formed in the hemopoietic organs together with other types of hemocytes (Akai and Sato, 1971;Han et al, 1998) and are also supplied by the division of circulating cells and by prohemocyte differentiation (Yamashita and Iwabuchi, 2001). Here we describe the effects of bombyxin-II on each B. mori cell type, particularly granulocytes, in detail.…”

Section: Introductionmentioning

confidence: 99%

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Effect of bombyxin-II, an insulin-related peptide of insects, on Bombyx mori hemocyte division in single-cell culture

Saito

Iwabuchi

2003

Appl. Entomol. Zool.

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We examined the effect of an insulin-related peptide, bombyxin-II, on the mitotic division of Bombyx mori hemocytes in vitro. Hemocytes isolated from larval hemolymph (day 1 of 4th instar) were cultured singly in MGM-450 medium. Some cultured prohemocytes (11.1%), plasmatocytes (2.6%), granulocytes (8.5%) and spherulocytes (10.8%) underwent mitotic division in the medium. Synthetic bombyxin-II added to the medium together with 10% larval hemolymph notably increased the ratio of dividing granulocytes to 45.5%, while no effect was evident on the other types of hemocytes. Bombyxin-II or hemolymph alone failed to stimulate the division of granulocytes. These results suggest that bombyxin-II acts cooperatively with hemolymph factor(s) to stimulate mitotic division of granulocytes. Bovine pancreas insulin was also active and could substitute for bombyxin.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of bombyxin-II, an insulin-related peptide of insects, on Bombyx mori hemocyte division in single-cell culture

Saito

Iwabuchi

2003

Appl. Entomol. Zool.

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“…1) e posterior extrusão do seu núcleo (EN, Fig. 1 BARDUCO et al, 1988;SAXENA et al, 1988;GENG & DUNN, 1989;BOMBONATO & GREGÓRIO, 1995;RIBEIRO et al, 1996;STRAND & JOHNSON, 1996;RICHARDS & EDWARDS, 1999;YAMASHITA & IWABUCHI, 2001. Fato interessante foi o observado para os oenocitóides.…”

Section: Resultsunclassified

“…Os resultados obtidos pela CDH são concordantes com as informações constantes na literatura que apontam PL e GR, como os hemócitos mais abundantes em várias espécies de insetos (GUPTA, 1979(GUPTA, , 1985RIBEIRO et al, 1996;RICHARDS & EDWARDS, 1999;YAMASHITA & IWABUCHI, 2001). Também foram concordantes com os encontrados por ANDRADE et al (2003) para as LS.…”

Section: Resultsunclassified

“…Os resultados apresentados por estes hemócitos em LR estão em concordância com o descrito por inúmeros autores (GUPTA, 1979(GUPTA, , 1985GUPTA & SUTHERLAND, 1966;KURIHARA et al, 1992a;AHMAD, 1993;BOMBONATO & GREGÓRIO, 1995;YAMASHITA & IWABUCHI, 2001;FALLEIROS et al, 2003) para outras espécies, bem como o descrito para LS de A. gemmatalis por ANDRADE et al (2003).…”

Section: Resultsunclassified

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Caracterização citológica dos hemócitos de Anticarsia gemmatalis (Lepidoptera, Noctuidae) em larvas resistentes ao vírus AgMNPV

Negreiro

Carvalho

Andrade

et al. 2009

Iheringia, Sér. Zool.

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ABSTRACT. Citological characterization of the Anticarsia gemmatalis (Lepidoptera, Noctuidae) hemocytes in resistant larvae to the virus AgMNPV. The occurrence of Anticarsia gemmatalis (Hübner, 1932) larvae resistant to the virus AgMNPV in laboratory led to the study of the hemocytes of this insect, in order to evalue its participation in the mechanisms which enables the resistance to the virus. The resistant larvae with 6 to 11 developmental days old (3 rd to 5 th instars) were anesthetized by cold and after that were quickly cleaned in 70% alcohol. The hemolymph was collected through abdominal puncturing; the morphological analysis was made in phase contrast and colored smears with Seller solution. The total hemocytes counting (THC), was made in modified Neubauer chamber with not diluted hemolymph. For the differential hemocytes counting (DHC), was used diluted hemolymph in anticoagulant solution for insects. Six hemocytes types were identified: plasmatocytes (38.5%), granulocytes (22.6%), oenocytoids (20.4%), spherulocytes (14.5%), prohemocytes (2.3%) and vermiform (1.5%). The total number of hemocytes showed a significant increase during the studied larval period.

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Correlation of hemocyte counts with different developmental parameters during the last larval instar of the tobacco hornworm, Manduca sexta

Beetz

Holthusen

Koolman

et al. 2007

Arch Insect Biochem Physiol

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We determined the changes in hemocyte titer and in the abundance of hemocyte types of the tobacco hornworm Manduca sexta during the fourth and fifth larval stadium and the beginning of the pupal stadium. As we analyzed the samples of individual insects at daily intervals, we were able to correlate phenotypical features, body weight, as well as total protein content and lysozyme activity in the hemolymph with the observations on hemocytes. In the course of the fifth larval stadium, the hemocyte titer decreased slightly and declined further after pupation. Using calculated values for total hemocyte numbers, females had about five times and males three times more hemocytes in the circulating population at the beginning of the wandering stage (in the middle of the fifth larval stadium) than immediately after the last larval--larval molt (from the fourth to the fifth larval stadium). This sexual difference was mainly due to an increase in the number of plasmatocytes, which was more prominent in females than in males. Granular cells were dominant in early fifth larval stadium while plasmatocytes were the most abundant cells in pupae. Oenocytoids and spherule cells disappeared during the wandering stage. Lysozyme activity in the hemolymph rose to a maximum during the wandering stage, with females having lysozyme values twice as high as those for males. These changes in lysozyme activity, however, did not correlate with the increase of total hemolymph protein titer which occurred already at the beginning of the wandering stage. We postulate that changes in hemocyte titers are under direct hormonal control, which has to be proven in future experiments.

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Bombyx mori prohemocyte division and differentiation in individual microcultures

Cited by 87 publications

References 21 publications

Effect of bombyxin-II, an insulin-related peptide of insects, on Bombyx mori hemocyte division in single-cell culture

Effect of bombyxin-II, an insulin-related peptide of insects, on Bombyx mori hemocyte division in single-cell culture

Caracterização citológica dos hemócitos de Anticarsia gemmatalis (Lepidoptera, Noctuidae) em larvas resistentes ao vírus AgMNPV

Correlation of hemocyte counts with different developmental parameters during the last larval instar of the tobacco hornworm, Manduca sexta

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