Bombesin receptor gene expression in rat pancreatic acinar AR42J cells: Transcriptional regulation by glucocorticoids

Retinoic acid receptor α mediates growth inhibition by retinoids in rat pancreatic carcinoma DSL-6A/C1 cells

“…Germany) according to the instructions of the manufacturer. Qualitative analysis of RNA was perforned using Northem blot analysis as previously described (Rosewicz et al 1994). RNA was denatured in a buffer containgno 6% s/v formaldehyde and 5% v/v formamide.…”

Transcriptional regulation of carbonic anhydrase II by retinoic acid in the human pacreatic tumor cell line DANG

“…RNA was denatured by formaldehyde, subjected to elctrophoresis through a 1% agarose gel in the presence of formaldehyde and then transferred onto nitrocellulose. Prehybridisation, hybridisation and washing procedures were carried out exactly as previously described [7], using a random primed cDNA probe for human CA II [8]. All filters were then sequentially hybridised with a cDNA encoding human flactin.…”

“…All filters were then sequentially hybridised with a cDNA encoding human flactin. Repeated quantitative analysis was performed by slot-blotting [7]. The hybridisation signal was quantitated by laser densitometry, normalised to fl-actin and then expressed as % of control.…”

“…Care was taken that each experimental condition contained the same amount of nuclei and radioactivity. Prehybridisation, hybridisation and washing procedures were carried out exactly as previously described [7].…”

Induction of matrix metalloprotease-1 gene expression by retinoic acid in the human pancreatic tumour cell line Dan-G

“…RNA was denatured by formaldehyde, subjected to electrophoresis through a 1% agarose gel in the presence of formaldehyde and then transferred onto nitrocellulose. Prehybridization, hybridization and washing procedures were carried out exactly as previously described (Rosewicz et al, 1994), using a random primed cDNA probe for human MMP-1. All filters were stripped and then sequentially hybridized with a cDNAencoding human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which was used as a loading control.…”