Bombesin-like peptide receptors in human bronchial epithelial cells

Kane, Madeleine A.; Toi-Scott, Miho; Johnson, Gary L.; Kelley, Kim; Boose, Dorothy; Escobedo-Morse, Antonio

doi:10.1016/0196-9781(95)02088-8

Cited by 16 publications

(10 citation statements)

References 15 publications

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“…Binding studies and Northern blot analysis have shown different predominant tissue distributions for GRP (brain, GI tract), neuromedin B (esophagus), and BRS-3 receptor (reproductive organs) subtypes [10][11][12]. Although no BLP receptors were found in lung tissue by Northern blot analysis [12], we have found significant levels of GRP specific binding, as well as mRNA as determined by reverse transcription (RT)-PCR for all three human BLP receptors in normal human lung tissue, indicating low levels of expression of all three [13]. Various lung cancer cell lines express one, two, three, or no known BLP receptor subtypes [14].…”

Section: Introductionmentioning

confidence: 84%

“…QC PCR was performed to obtain quantitative estimates of mRNA expression using an internal standard prepared from GRP receptor-specific sequences on its ends according to the vendor's protocol and the MIMIC procedure as previously described [13,37]. The concentration of the stock internal standard was 3 mM as determined by spectrophotometry after purification by ethanol precipitation.…”

Section: Quantitative Competitive (Qc) Pcrmentioning

confidence: 99%

“…Reverse transcription was performed as previously described with AMV reverse transcriptase for 90 min at 42 • C [13,14,37]. PCR for GRP receptor was performed with specific primers as previously described [13,14,35].…”

Section: Rt-pcrmentioning

confidence: 99%

“…SHP77 cells and their conditioned medium were extracted in 2 M acetic acid and BLP analysis was performed by ELISA as previously described [13,28]. A standard curve was included with every assay.…”

Section: Blp Immunoassaymentioning

confidence: 99%

“…Triton-solubilized membrane extracts were prepared from cells, and specific GRP binding assays as a measure of total cellular GRP receptor were performed by gel filtration chromatography as previously described [13,34].…”

Section: Specific [ 125 I]-grp Binding Assaysmentioning

confidence: 99%

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Dexamethasone regulation of gastrin-releasing peptide receptor in human lung cells

et al. 2004

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Section: Introductionmentioning

confidence: 84%

Section: Quantitative Competitive (Qc) Pcrmentioning

confidence: 99%

Section: Rt-pcrmentioning

confidence: 99%

Section: Blp Immunoassaymentioning

confidence: 99%

Section: Specific [ 125 I]-grp Binding Assaysmentioning

confidence: 99%

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Dexamethasone regulation of gastrin-releasing peptide receptor in human lung cells

et al. 2004

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68Ga-Labeled Bombesin Analogs for Receptor-Mediated Imaging

Varshney

Hazari

Fernandez

et al. 2012

Recent Results in Cancer Research

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Targeted receptor-mediated imaging techniques have become crucial tools in present targeted diagnosis and radiotherapy as they provide accurate and specific diagnosis of disease information. Peptide-based pharmaceuticals are gaining popularity, and there has been vast interest in developing (68)Ga-labeled bombesin (Bn) analogs. The gastrin-releasing peptide (GRP) family and its Bn analog have been implicated in the biology of several human cancers. The three bombesin receptors GRP, NMB, and BRS-3 receptor are most frequently ectopically expressed by common, important malignancies. The low expression of Bn/GRP receptors in normal tissue and relatively high expression in a variety of human tumors can be of biological importance and form a molecular basis for Bn/GRP receptor-mediated imaging. To develop a Bn-like peptide with favorable tumor targeting and pharmacokinetic characteristics for possible clinical use, several modifications in the Bn-like peptides, such as the use of a variety of chelating agents, i.e., acyclic and macrocyclic agents with different spacer groups and with different metal ions (gallium), have been performed in recent years without significant disturbance of the vital binding scaffold. The favorable physical properties of (68)Ga, i.e., short half-life, and the fast localization of small peptides make this an ideal combination to study receptor-mediated imaging in patients.

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