Bolus Oral or Continuous Intestinal Amino Acids Reduce Hypothermia during Anesthesia in Rats

Imoto, Akinobu; Yokoyama, Takeshi; Suwa, Kunio; Yamasaki, Fumiyasu; Yatabe, Tomoaki; Yokoyama, Reiko; Yamashita, Koichi; Selldén, Eva

doi:10.3177/jnsv.56.104

Cited by 7 publications

(10 citation statements)

References 26 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the sham-operated rats, however, the inhibitory effect of amino acid infusion on hypothermia was not seen. In other studies done in male rats, amino acid infusion had a significant inhibitory effect on hypothermia, as measured by central body temperature, during general anesthesia (7,8,12,13). The results of our study suggest that in rats, ovarian function or female hormone decreases the inhibitory effect of amino acid infusion on anesthesia-induced hypothermia.…”

Section: Discussionsupporting

confidence: 58%

Effects of Intravenous Amino Acids on Anesthesia-Induced Hypothermia in Ovariectomized Rats

Ando

Kanazawa

Tsuda

et al. 2012

J Nutr Sci Vitaminol

View full text Add to dashboard Cite

Summary A decrease in core temperature during general anesthesia is attenuated by infusion of an intravenous amino acid mixture. The purpose of the present study was to investigate the influence of physical and endocrine changes caused by ovariectomy on the inhibitory effect of amino acid infusion on anesthesia-induced hypothermia. Sprague-Dawley female rats were divided into a sham-operated (Sham) group and an ovariectomized (OVX) group. Saline solution or an amino acid mixture solution was infused for 180 min during sevoflurane anesthesia, and the rectal temperature was measured (4 groups). Intraperitoneal white adipose tissue mass, bilateral gastrocnemius weight and plasma insulin levels were measured. In the Sham rats, no inhibitory effect of the amino acid mixture on anesthesia-induced hypothermia was found, while in the OVX rats, hypothermia was significantly decreased. The intraperitoneal fat weight/body weight ratio was significantly higher in the OVX rats than in the Sham rats, but the gastrocnemius weight/body weight ratio was not significantly different. After amino acid infusion, the plasma insulin level was significantly higher in the OVX rats than in the Sham rats. In conclusion, our findings suggest that, in rats, ovarian function or female hormone affects protein turnover mediated by increase in insulin secretion and, thus, decreases the inhibitory effect of an infusion of amino acid mixture on anesthesia-induced hypothermia. Key Words hypothermia, amino acids, general anesthesia, ovariectomy Redistribution hypothermia occurs in the body after induction of general anesthesia. Core body temperature gradually declines as heat is lost from the body surface, and heat production slows because of decreased metabolism. Selldén et al. reported that an amino acid infusion during anesthesia caused nutrient-induced thermogenesis (NIT), preventing the temperature reduction of redistribution hypothermia ( 1 ). Fujiwara et al. noted a similar effect after amino acid infusion but not after an infusion of glucose and fat emulsion ( 2 ). It is known that the metabolism of amino acids requires a large expenditure of energy ( 3 ), and protein synthesis induced by amino acids has been shown to correlate positively with energy expenditure ( 4 ). When rats were given the protein synthesis inhibitor puromycin, NIT did not occur after amino acid infusion ( 5 ). It has also been suggested that 75% of the increased oxygen uptake by amino acid infusion is produced in tissues other than the internal organs ( 6 ). Amino acid infusion during general anesthesia was found to stimulate protein synthesis in skeletal muscle via enhanced insulin secretion and to prevent hypothermia by heat accumulation through NIT ( 7 ). We have reported that skeletal muscle and plasma insulin levels play a major role in changes in body temperature during general anesthesia and the effect of amino acids on body temperature in a rat model of muscle atrophy ( 8 ).On the other hand, sex hormone is known to be involved in thermoregulation. In addition,...

show abstract

Section: Discussionsupporting

confidence: 58%

Effects of Intravenous Amino Acids on Anesthesia-Induced Hypothermia in Ovariectomized Rats

Ando

Kanazawa

Tsuda

et al. 2012

J Nutr Sci Vitaminol

View full text Add to dashboard Cite

show abstract

“…Intraoperative amino acid infusion exerts a marked thermogenic effect with enhanced oxygen consumption [2,3,4,5], mainly in the extra-splanchnic tissues, and significantly attenuates the decrease in core temperature during general anesthesia [6,7]. …”

Section: Introductionmentioning

confidence: 99%

Metabolic Effects of Intraoperative Amino Acid Infusion in Mongrel Dogs

Lin

Wang³

et al. 2012

Ann Nutr Metab

View full text Add to dashboard Cite

Background: Intraoperative amino acid infusion can attenuate the decrease in core temperature, but the metabolic effects are uncertain. Methods: Thirty-six healthy mongrel dogs undergoing ileectomy under general anesthesia were infused intraoperatively with normal saline or 18 compound amino acids at 6, 12, and 24 kJ·kg^–1·h^–1 (NS, 6-, 12-, and 24-kJ groups) and studied until 24 h after the operation. Blood glucose, plasma insulin, free fatty acids, and triglyceride concentrations were determined at 7 defined time points. Muscle aminograms, urinary urea, and 3-methylhistidine excretions were measured before and after the operation. Results: Blood glucose and plasma insulin increased amino acid dose dependently during the operation and in the early period after the operation. Free fatty acids were significantly lower in the 12- and 24-kJ groups compared with the NS group at the end of the operation. The negative nitrogen balance was alleviated dose dependently in the amino acid groups on operation day. The urinary 3-methylhistidine decreased significantly during the first 24 h after the operation in the 24-kJ group, while it increased in the other groups with the largest increase in the NS group. Basic, branched-chain, and aromatic amino acids in the vastus lateralis muscle increased dose dependently at the end of the operation in the amino acid groups. Conclusion: Intraoperative amino acid infusion has the dose-dependent effects of increasing blood glucose, inhibiting fat mobilization and muscle protein breakdown.

show abstract

“…However, rats in group C received 21 mL/kg of the carbohydrate-rich beverage, which corresponded to an energy content of 70.6 kJ/kg. In a previous study, we administered 70.6 kJ/kg of amino acid to rats and observed that this dose prevented hypothermia [10]. Although this dose seems much larger than the doses appropriate for humans, it should be considered that the rat metabolism is 5-7 times higher than that of humans, and that protein consumption by weight in rats is approximately 7 times that in humans [10].…”

Section: Methodsmentioning

confidence: 96%

“…Selldén et al [8,9] used another approach and reported that intravenous amino acid infusion prevents intraoperative hypothermia under general and spinal anesthesia. We also reported that preoperative oral amino acid intake contributes to the prevention of intraoperative hypothermia under general anesthesia in rats [10]. Mizobe et al [11] reported that preoperative fructose infusion helped to maintain normothermia by augmenting metabolic heat production and increasing the vasoconstriction threshold.…”

Section: Introductionmentioning

confidence: 94%

Preoperative carbohydrate-rich beverage reduces hypothermia during general anesthesia in rats

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

Bolus Oral or Continuous Intestinal Amino Acids Reduce Hypothermia during Anesthesia in Rats

Cited by 7 publications

References 26 publications

Effects of Intravenous Amino Acids on Anesthesia-Induced Hypothermia in Ovariectomized Rats

Effects of Intravenous Amino Acids on Anesthesia-Induced Hypothermia in Ovariectomized Rats

Metabolic Effects of Intraoperative Amino Acid Infusion in Mongrel Dogs

Preoperative carbohydrate-rich beverage reduces hypothermia during general anesthesia in rats

Contact Info

Product

Resources

About