Bollworm responses to release of genetically modified Helicoverpa armigera nucleopolyhedroviruses in cotton

Sun, Xiulian; Chen, Xinwen; Zhang, Zhongxin; Wang, Hualin; Bianchi, Felix J.J.A.; Peng, Haiyi; Vlak, Just M.; Hú, Zhìhóng

doi:10.1016/s0022-2011(02)00144-1

Cited by 52 publications

(28 citation statements)

References 33 publications

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“…Among these approaches, the expression of insect-specific scorpion neurotoxin, AaIT, derived from the North African scorpion, resulted in a significant increase in the pathogenicity of baculovirus (9,10). To date, all of the field trials using recombinant AcMNPV constructs expressing AaIT have shown a reduced level of feeding damage in comparison to wild-type AcMNPV-treated control plants (6,(27)(28)(29). Thus, in the present study, the AaIT gene was selected for the construction of NeuroBactrus.…”

Section: Discussionmentioning

confidence: 99%

NeuroBactrus, a Novel, Highly Effective, and Environmentally Friendly Recombinant Baculovirus Insecticide

Shim

Choi

Wang

et al. 2013

Appl Environ Microbiol

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A novel recombinant baculovirus, NeuroBactrus, was constructed to develop an improved baculovirus insecticide with additional beneficial properties, such as a higher insecticidal activity and improved recovery, compared to wild-type baculovirus. For the construction of NeuroBactrus, the Bacillus thuringiensis crystal protein gene (here termed cry1-5) was introduced into the Autographa californica nucleopolyhedrovirus (AcMNPV) genome by fusion of the polyhedrin-cry1-5-polyhedrin genes under the control of the polyhedrin promoter. In the opposite direction, an insect-specific neurotoxin gene, AaIT, from Androctonus australis was introduced under the control of an early promoter from Cotesia plutellae bracovirus by fusion of a partial fragment of orf603. The polyhedrin-Cry1-5-polyhedrin fusion protein expressed by the NeuroBactrus was not only occluded into the polyhedra, but it was also activated by treatment with trypsin, resulting in an ϳ65-kDa active toxin. In addition, quantitative PCR revealed that the neurotoxin was expressed from the early phase of infection. NeuroBactrus showed a high level of insecticidal activity against Plutella xylostella larvae and a significant reduction in the median lethal time against Spodoptera exigua larvae compared to those of wild-type AcMNPV. Rerecombinant mutants derived from NeuroBactrus in which AaIT and/or cry1-5 were deleted were generated by serial passages in vitro. Expression of the foreign proteins (B. thuringiensis toxin and AaIT) was continuously reduced during the serial passage of the NeuroBactrus. Moreover, polyhedra collected from S. exigua larvae infected with the serially passaged NeuroBactrus showed insecticidal activity similar to that of wild-type AcMNPV. These results suggested that NeuroBactrus could be recovered to wild-type AcMNPV through serial passaging.

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Section: Discussionmentioning

confidence: 99%

NeuroBactrus, a Novel, Highly Effective, and Environmentally Friendly Recombinant Baculovirus Insecticide

Shim

Choi

Wang

et al. 2013

Appl Environ Microbiol

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“…It has already been proven that they can effectively replace chemical insecticides in the field, for example, in the case of Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) sprayed on cotton fields in Australia (9) and China (38) to control one of the most widely spread polyphagous pests (10). Baculoviruses occur naturally, are nonpathogenic to humans or other vertebrates, and are relatively host specific, and no impact on nontarget organisms has been reported to date.…”

mentioning

confidence: 99%

Downregulation of a Chitin Deacetylase-Like Protein in Response to Baculovirus Infection and Its Application for Improving Baculovirus Infectivity

Jakubowska

Caccia

Gordon

et al. 2010

J Virol

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Several expressed sequence tags (ESTs) with homology to chitin deacetylase-like protein (CDA) were selected from a group of Helicoverpa armigera genes whose expression changed after infection with H. armigera single nucleopolyhedrovirus (HearNPV). Some of these ESTs coded for a midgut protein containing a chitin deacetylase domain (CDAD). The expressed protein, HaCDA5a, did not show chitin deacetylase activity, but it showed a strong affinity for binding to chitin. Sequence analysis showed the lack of any chitin binding domain, described for all currently known peritrophic membrane (PM) proteins. HaCDA5a has previously been detected in the H. armigera PM. Such localization, together with its downregulation after pathogen infection, led us to hypothesize that this protein might be responsible for the homeostasis of the PM structure and that, by reduction of its expression, the insect may reduce PM permeability, decreasing the entrance of baculovirus. To test this hypothesis, we constructed a recombinant nucleopolyhedrovirus to express HaCDA5a in insect cells and tested its influence on PM permeability as well as the influence of HaCDA5a expression on the performance of the baculovirus. The experiments showed that HaCDA5a increased PM permeability, in a concentration-dependent manner. Bioassays on Spodoptera frugiperda and Spodoptera exigua larvae revealed that NPV expressing HaCDA5a was more infective than its parental virus. However, no difference in virulence was observed when the viruses were injected intrahemocoelically. These findings support the downregulation of a midgut-specific CDA-like protein as a possible mechanism used by H. armigera to reduce susceptibility to baculovirus by decreasing PM permeability.

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“…One of the most successful examples of using wild-type baculovirus (Anticarsia gemmatalis NPV) was in Brazil, for control of the velvet bean caterpillar in soybean crops (Moscardi, 1999). Another successful example was in China for control of Helicoverpa armigera on cotton (Sun et al, 2002). But there are still limitations associated with the use of baculoviruses as insecticides, such as 1) problems with long-term storage, 2) high cost of production relative to classical chemical pesticide, 3) narrow host range and 4) relatively slow speed of kill.…”

Section: Limitations Of Using Baculoviruses As Insecticidesmentioning

confidence: 99%

Tissue specificity of a baculovirus-expressed, basement membrane-degrading protease in larvae of Heliothis virescens

Tang

Lei

et al. 2007

Tissue and Cell

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Bollworm responses to release of genetically modified Helicoverpa armigera nucleopolyhedroviruses in cotton

Cited by 52 publications

References 33 publications

NeuroBactrus, a Novel, Highly Effective, and Environmentally Friendly Recombinant Baculovirus Insecticide

NeuroBactrus, a Novel, Highly Effective, and Environmentally Friendly Recombinant Baculovirus Insecticide

Downregulation of a Chitin Deacetylase-Like Protein in Response to Baculovirus Infection and Its Application for Improving Baculovirus Infectivity

Tissue specificity of a baculovirus-expressed, basement membrane-degrading protease in larvae of Heliothis virescens

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