Bolidophyceae, a Sister Picoplanktonic Group of Diatoms – A Review

Kuwata, Akira; Yamada, Kazumasa; Ichinomiya, Mutsuo; Yoshikawa, Shinya; Tragin, Margot; Vaulot, Daniel; Santos, Adriana Lopes dos

doi:10.3389/fmars.2018.00370

Cited by 31 publications

(49 citation statements)

References 105 publications

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“…Among Dictyochophyceae, the main ASV matches with 97.7% similarity Helicopedinella tricostata and with higher similarity (99.2%) an undescribed strain (RCC2289) isolated from the Arctic (Balzano et al 2012a), suggesting that this ASV may correspond to a new species or even genus, while some of the other ASVs match the species Florenciella parvula and Pseudochattonella farcimen. Bolidophyceae were represented by Triparma laevis as well as environmental clades (Kuwata et al 2018). One uncultivated group MOCH-2 (Marine OCHrophyta, Massana et al 2014) was found in many filtered and sorted samples although at low abundance.…”

Section: /58mentioning

confidence: 99%

Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula

Trefault

Iglesia

Moreno-Pino

et al. 2020

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Year-round reports of phytoplankton dynamics in the West Antarctic Peninsula are rare and mainly limited to microscopy and/or pigment-based studies. We analyzed the phytoplankton community from coastal waters of Fildes Bay in the West Antarctic Peninsula between January 2014 and 2015 using metabarcoding of the nuclear and plastidial 18/16S rRNA gene from both size-fractionated and flow cytometry sorted samples. Each metabarcoding approach yielded a different image of the phytoplankton community with for example Prymnesiophyceae more prevalent in plastidial metabarcodes and Mamiellophyceae in nuclear ones. Overall 14 classes of photosynthetic eukaryotes were present in our samples with the following dominating: Bacillariophyta (diatoms), Pelagophyceae and Dictyochophyceae for division Ochrophyta, Mamiellophyceae and Pyramimonadophyceae for division Chlorophyta, Prymnesiophyceae and Cryptophyceae. Diatoms were dominant in the larger size fractions and during summer, while Prymnesiophyceae and Cryptophyceae were dominant in colder seasons. Pelagophyceae were particularly abundant towards the end of autumn (May). In addition of Micromonas polaris and Micromonas sp. clade B3, both previously reported in Arctic waters, we detected a new Micromonas 18S rRNA sequence signature, close to but clearly distinct from M. polaris, which potentially represent a new clade specific of the Antarctic. These results highlight the need for complementary strategies as well as the importance of year-round monitoring for a comprehensive description of phytoplankton communities in Antarctic coastal waters.

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Section: /58mentioning

confidence: 99%

Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula

Trefault

Iglesia

Moreno-Pino

et al. 2020

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“…The OSD metabarcoding dataset is invaluable to determine the distribution of many phytoplankton groups in coastal waters 9,11,57 . However it must be always emphasized that metabarcoding is not a quantitative method because of biases in the PCR reaction and of the variation in the number of 18S rRNA gene copies per organism 58 However in the case of Mamiellophyceae, because their size is small, the number of copies is low 58 and does not vary too much between the different genera (e.g.…”

Section: Limitations Of the Osd Datasetmentioning

confidence: 99%

Novel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding

Tragin

Vaulot

2018

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The Ocean Sampling Day (OSD) project provided metabarcoding data for the V4 hyper-variable regions of the 18S rRNA gene from 157 samples collected at 143 mostly coastal stations. In this paper we focus on the class Mamiellophyceae, which was found at nearly all OSD stations and represented 55 % of the green microalgae (Chlorophyta) reads in the 2014 OSD dataset. We performed phylogenetic analyses of unique OSD metabarcodes (ASV, amplicon single variants) and reference GenBank sequences from cultures and from the environment, focusing on the four most represented genera: Ostreococcus (45 % of the Mamiellophyceae reads), Micromonas (34 %), Bathycoccus (10 %) and Mantoniella (8.7 %).These analyses uncovered novel diversity within each genus except Bathycoccus. In Ostreococcus, a new clade (E) with 2 very clear base pair differences compared to the oceanic clade B in the V4 region was the second most represented clade after the coastal Ostreococcus "lucimarinus". Within Micromonas, ten clades were found exceeding the 4 species and 2 candidate species already described. Finally, we found 2 new environmental clades of Mantoniella. Each Mamiellophyceae clade had a specific distribution in the OSD dataset suggesting that they are adapted to different ecological niches. Monomastigales 10 . Monomastigales are confined to freshwater environments while Dolichomasti-20 gales, although quite diversified in marine waters 12, 13 , are a minor component of Mamiel-21 lophyceae in coastal waters. In contrast, Mamiellales and Bathycoccales host some of the most 22 common Chlorophyta microalgae such as the ubiquitous Micromonas, the smallest known eu-23 karyote Ostreococcus or the coccoid Bathycoccus 10 . Within Mamiellales, Micromonas pusilla 14 24 was recently split into four species: Micromonas bravo (previously clade B.E.3), Micromonas com-25 moda (previous clade A.ABC.1-2), Micromonas polaris (previously clade B arctic), Micromonas 26 pusilla (previously clade C.D.5) and two clades mentioned as candidate species 1 (clade B. .4) 27 2/34 and candidate species 2 (clade B warm) 15 . Within the genus Mantoniella, only two species 28 have been described: the ubiquitous Mantoniella squamata 3, 16 , first described as Micromonas 29 squamata 17 , and Mantoniella antarctica 18 . Within Bathycoccales, four Ostreococcus clades have 30 been delineated 19 : Ostreococcus tauri 20 , Ostreococcus mediterraneus 21 , both of which have 31 been formerly described, Ostreococcus "lucimarinus" (clade A) and clade B, which both lack 32 formal taxonomic description. Analyses of pigment content and response to light levels allowed 33 to distinguish two broad ecotypes: strains adapted to high (O. tauri,O. mediterraneus, O. "luci-34 marinus" ) and low (Ostreococcus clade B) light 22 . The second genus within Bathycoccales hosts 35 a single species, Bathycoccus prasinos 23 . No clades can be delineated inside this species based 36 on 18S rRNA gene sequences from cultures and the environment. However, divergence in ITS 37 sequences suggest that Bathycoccus p...

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“…The phylogenetic analysis recovered that the White Sea bolidophytes refer to three environmental clades (env. clades I, II and III [88] (Figure 4)), in addition to the group corresponding to the genus Triparma. Bolidophyte T. strigata has a worldwide distribution in plankton and is most abundant in polar waters [89][90][91].…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic Picoeukaryotes Diversity in the Underlying Ice Waters of the White Sea, Russia

et al. 2020

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The White Sea is a unique basin combining features of temperate and arctic seas. The current state of its biocenoses can serve as a reference point in assessing the expected desalination of the ocean as a result of climate change. A metagenomic study of under-ice ice photosynthetic picoeukaryotes (PPEs) was undertaken by Illumina high-throughput sequencing of the 18S rDNA V4 region from probes collected in March 2013 and 2014. The PPE biomass in samples was 0.03–0.17 µg C·L−1 and their abundance varied from 10 cells·mL−1 to 140 cells·mL−1. There were representatives of 16 algae genera from seven classes and three supergroups, but Chlorophyta, especially Mamiellophyceae, dominated. The most represented genera were Micromonas and Mantoniella. For the first time, the predominance of Mantoniella (in four samples) and Bolidophyceae (in one sample) was observed in under-ice water. It can be assumed that a change in environmental conditions will lead to a considerable change in the structure of arctic PPE communities.

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Bolidophyceae, a Sister Picoplanktonic Group of Diatoms – A Review

Cited by 31 publications

References 105 publications

Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula

Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula

Novel diversity within marine Mamiellophyceae (Chlorophyta) unveiled by metabarcoding

Photosynthetic Picoeukaryotes Diversity in the Underlying Ice Waters of the White Sea, Russia

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