BoLA-DRB3 exon 2 mutations associated with paratuberculosis in cattle

Mucha, Rastislav; Mangesh, Bhide

doi:10.1016/j.tvjl.2011.07.005

Cited by 14 publications

(10 citation statements)

References 9 publications

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“…A potential limitation of such approach is that the MHC-DR PBR positions used have been documented by X-ray crystallography just in humans and mice. Nevertheless, several of these positions have been associated with vaccine responses and susceptibility or resistance to infectious diseases (Garcia-Briones et al, 2000;Baxter et al, 2009;Rastislav and Mangesh, 2012;Carignano et al, 2017) and thus analysis based on these positions is reasonable.…”

Section: Discussionmentioning

confidence: 99%

Major Histocompatibility Complex Class II (DRB3) Genetic Diversity in Spanish Morucha and Colombian Normande Cattle Compared to Taurine and Zebu Populations

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Major Histocompatibility Complex Class II (DRB3) Genetic Diversity in Spanish Morucha and Colombian Normande Cattle Compared to Taurine and Zebu Populations

et al. 2020

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“…This conservation extends to the breeds studied here, and is particularly evident in aminoacids at positions 70, 71, and 74 of pocket 4, which occupies a central position within the peptide binding groove. These aminoacids have been implicated as playing a signi cant role in human immune responsiveness (58), but also correlated with vaccine responses and infectious disease susceptibility or tolerance in bovines (55,(59)(60)(61). In this sense, it could be hypothesized that eventhough bovine breeding programs make use of extensive crossing, there may be a functional constraint in the PBR to BoLA-DRB3 aminoacid variability.…”

Section: Discussionmentioning

confidence: 99%

Diversity of the BoLA-DRB3 Gene in Cattle Breeds From Tropical and Subtropical Regions of South America

Valenzano¹,

Caffaro²,

Lia³

et al. 2021

Preprint

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Background Bovine leukocyte antigens (BoLA) are widely used as markers of disease and immunological traits in cattle because of their primary function in the recognition of pathogens by the immune system. To date, however, the characterization of BoLA gene polymorphisms in Latin American Zebu and mixed zebuine breeds is scarce. By a sequence-based typing method, here we sequenced exon 2 of BoLA class II DRB3 gene of 264 animals from the five breeds (Creole, Brahman, Braford, Brangus and Nellore) most commonly used in northern Argentina. Results The taurine and zebuine breeds contained 61 previously reported alleles. Genetic diversity was high at allelic and molecular levels, particularly in the mixed breeds. Neutrality tests revealed that none of the breeds shows the even gene frequency distribution expected under a balanced selection scenario. The FST index shows significant differences across all populations (FST=0.052, P < 0.001), indicating a substantial differentiation between breeds. UPGMA trees and Metric multidimensional scaling (MDS) analysis show that, as expected, Creole is distantlyrelated to the other zebuinebreeds. Among them, Brahman, Braford and Brangus cluster closer together than with Nellore. These data are consistent with the historical and geographical origin of these breeds. A peptide binding region sequence analysis at the aminoacid level revealed that, despite the nucleotide diversity among the breeds, the key aminoacids involved in peptide recognition are greatly conserved. Conclusions This is the first report of BoLA-DRB3 diversity in pure and mixed zebuine cattle breeds from Argentina. Knowledge ofBoLA-DRB3 variability in breeds adapted to tropical and subtropical environments contributes not only to ongoing efforts to catalog bovine MHC allele frequencies by breed and location but also to the design of peptide-based vaccines.

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“…(2005) using microsatellite markers closely associated with MHC region. Recently, Rastislav & Mangesh (2011) reported the association of an SNP within the same DRB gene with susceptibility to paratuberculosis in cattle.…”

Section: Discussionmentioning

confidence: 99%

Effect of genetic variation in the MHC Class II DRB region on resistance and susceptibility to Johne’s disease in endangered Indian Jamunapari goats

Singh

et al. 2012

Int J Immunogenetics

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The pathogenesis of Johne's disease (JD), caused by Mycobacterium avium subsp. paratuberculosis (MAP), is complex and has not been completely understood yet. In the present study, we analysed the polymorphism in the exon-2 of the caprine major histocompatibility complex (MHC) Class II DRB region and its association with resistance or susceptibility to JD. A total of 203 Jamunapari goats, which is an Indian endangered breed highly susceptible to JD, kept at a single farm were studied. On the basis of clinical signs, microscopic examination, faecal culture, ELISA and diagnostic PCR, 60 and 143 goats were classified as resistant and susceptible to JD, respectively. PCR-based restriction fragment length polymorphism (PCR-RFLP) with two enzymes, PstI and TaqI, was used to assess variation in the DRB gene(s) in all 203 goats studied. Two di-allelic single nucleotide polymorphisms (SNPs), here referred as 'P' and 'T', were tested. In each of them, three genotypes were found in the group analysed. The minimum allele frequencies (MAFs) were 0.233 and 0.486 for the P and T SNPs, respectively. Statistically significant associations between alleles, individual genotypes and composed genotypes of both SNPs were found. The frequency of p and t alleles, of individual pp and tt and of composed pptt genotypes were significantly higher (P(corr) < 0.001) in the 'resistant' group as compared to the 'susceptible' group, while the P and T alleles were associated with susceptibility (P(corr) < 0.001). In heterozygous genotypes, susceptibility was dominant over resistance. The effects of both SNP on resistance and susceptibility were comparable and composed heterozygous genotypes showed intermediate levels of susceptibility in terms of the odds ratio and P-values calculated.

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BoLA-DRB3 exon 2 mutations associated with paratuberculosis in cattle

Cited by 14 publications

References 9 publications

Major Histocompatibility Complex Class II (DRB3) Genetic Diversity in Spanish Morucha and Colombian Normande Cattle Compared to Taurine and Zebu Populations

Major Histocompatibility Complex Class II (DRB3) Genetic Diversity in Spanish Morucha and Colombian Normande Cattle Compared to Taurine and Zebu Populations

Diversity of the BoLA-DRB3 Gene in Cattle Breeds From Tropical and Subtropical Regions of South America

Effect of genetic variation in the MHC Class II DRB region on resistance and susceptibility to Johne’s disease in endangered Indian Jamunapari goats

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