BoLA class I allele diversity and polymorphism in a herd of cattle

Babiuk, Shawn; Horseman, Benjamin; Zhang, Chenhong; Bickis, Mik; Kusalik, Anthony; Schook, Lawrence B.; Abrahamsen, Mitchell S.; Pontarollo, Reno

doi:10.1007/s00251-006-0173-7

Cited by 21 publications

(20 citation statements)

References 30 publications

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“…Also, the chaperone heat shock cognate 70 kDa protein 8 (hsc70) and an inhibitor of cysteine and serine proteases, endopin-2 [12] belong to this fraction. Besides, two membrane-bound proteins, namely sodium/potassium-transporting ATPase subunit alpha-1, essential for osmoregulation [13], and MHC I heavy chain isoform 1, which is expressed on all mammalian nucleated cells [14], were identified (Table 1, proteins 12 and 14). …”

Section: Resultsmentioning

confidence: 99%

Bovine neonatal pancytopenia - Comparative proteomic characterization of two BVD vaccines and the producer cell surface proteome (MDBK)

Euler¹,

Hauck

Ueffing

et al. 2013

BMC Vet Res

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BackgroundBovine neonatal pancytopenia (BNP) is a disease syndrome in newborn calves of up to four weeks of age, first observed in southern Germany in 2006. By now, cases have been reported in several countries around the globe. Many affected calves die within days due to multiple haemorrhages, thrombocytopenia, leukocytopenia and bone marrow depletion. A certain vaccine directed against Bovine Virus Diarrhoea Virus (BVDV) was recently shown to be associated with BNP pathogenesis. Immunized cows develop alloantibodies that are transferred to newborn calves via colostrum intake. In order to further elucidate BNP pathogenesis, the purpose of this study was to characterize and compare the protein composition of the associated vaccine to another vaccine directed against BVDV not related to BNP and the cell surface proteome of MDBK (Madin-Darby Bovine Kidney) cells, the cell line used for production of the associated vaccine.ResultsBy SDS-PAGE and mass spectrometry, we were able to detect several coagulation-related and immune modulatory proteins, as well as cellular and serum derived molecules being shared between the associated vaccine and MDBK cells. Furthermore, the number of proteins identified in the BNP related vaccine was almost as high as the number of surface proteins detected on MDBK cells and exceeded the amount of proteins identified in the non-BNP related vaccine over 3.5 fold. The great amount of shared cellular and serum derived proteins confirm that the BNP associated vaccine contained many molecules originating from MDBK cells and vaccine production.ConclusionsThe respective vaccine was not purified enough to prevent the development of alloantibodies. To narrow down possible candidate proteins, those most likely to represent a trigger for BNP pathogenesis are presented in this study, giving a fundament for further analysis in future research.

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Section: Resultsmentioning

confidence: 99%

Bovine neonatal pancytopenia - Comparative proteomic characterization of two BVD vaccines and the producer cell surface proteome (MDBK)

Euler¹,

Hauck

Ueffing

et al. 2013

BMC Vet Res

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“…Examples include (1) humans, who express three classical loci, HLA-A , - B , and - C , and two, HLA-E and - F , or three with HLA-G , non-classical loci (Ober et al 1998); (2) pigs, which express up to three classical loci, SLA-1 , - 2 , and - 3 and three non-classical loci SLA-6 , - 7 , and - 8 (Smith et al 2005); (3) chickens, which express two classical loci, B–F major and minor and one non-classical locus, Y-F (Kaufman et al 1999); and (4) rats, which express one to three classical loci, RT1-A1 , - A2 , and - A3 (Joly et al 1996), and various combinations of non-classical loci, RT1-C, RT1-E, RT-BM1 and RT1-N1 (Kirisits et al 1992; Lau et al 2000). A pattern of variable numbers of expressed class I genes among MHC haplotypes has also been described for the rhesus macaque, pig, cow, and sheep (Babiuk et al 2007; Birch et al 2008; Ellis et al 1999; Miltiadou et al 2005; Otting et al 2005; Tanaka-Matsuda et al 2009). …”

Section: Introductionmentioning

confidence: 80%

Analysis of MHC class I genes across horse MHC haplotypes

et al. 2010

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The genomic sequences of 15 horse Major Histocompatibility Complex (MHC) class I genes and a collection of MHC class I homozygous horses of five different haplotypes were used to investigate the genomic structure and polymorphism of the equine MHC. A combination of conserved and locusspecific primers was used to amplify horse MHC class I genes with classical and non-classical characteristics. Multiple clones from each haplotype identified three to five classical sequences per homozygous animal, and two to three non-classical sequences. Phylogenetic analysis was applied to these sequences and groups were identified which appear to be allelic series, but some sequences were left ungrouped. Sequences determined from MHC class I heterozygous horses and previously described MHC class I sequences were then added, representing a total of ten horse MHC haplotypes. These results were consistent with those obtained from the MHC homozygous horses alone, and 30 classical sequences were assigned to four previously confirmed loci and three new provisional loci. The non-classical genes had few alleles and the classical genes had higher levels of allelic polymorphism. Alleles for two classical loci with the expected pattern of polymorphism were found in the majority of haplotypes tested, but alleles at two other commonly detected loci had more variation outside of the hypervariable region than within. Our data indicate that the equine Major Histocompatibility Complex is characterized by variation in the complement of class I genes expressed in different haplotypes in addition to the expected allelic polymorphism within loci.

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“…First, expressed MHC loci, which are among the most polymorphic loci in mammals, generate mRNA transcripts that display considerable nucleotide differences between individuals from the same species (69–71). mRNA transcripts that vary appreciably from the reference transcript sequences used to produce the microarray probe sets may, therefore, have a reduced hybridization efficiency compared to mRNA transcripts that are identical to or differ only slightly from the reference transcript sequences.…”

Section: The Monocyte-derived Macrophage Infection Model and Gene Expmentioning

confidence: 99%

Comparative Functional Genomics and the Bovine Macrophage Response to Strains of the Mycobacterium Genus

et al. 2014

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Mycobacterial infections are major causes of morbidity and mortality in cattle and are also potential zoonotic agents with implications for human health. Despite the implementation of comprehensive animal surveillance programs, many mycobacterial diseases have remained recalcitrant to eradication in several industrialized countries. Two major mycobacterial pathogens of cattle are Mycobacterium bovis and Mycobacterium avium subspecies paratuberculosis (MAP), the causative agents of bovine tuberculosis (BTB) and Johne’s disease (JD), respectively. BTB is a chronic, granulomatous disease of the respiratory tract that is spread via aerosol transmission, while JD is a chronic granulomatous disease of the intestines that is transmitted via the fecal-oral route. Although these diseases exhibit differential tissue tropism and distinct complex etiologies, both M. bovis and MAP infect, reside, and replicate in host macrophages – the key host innate immune cell that encounters mycobacterial pathogens after initial exposure and mediates the subsequent immune response. The persistence of M. bovis and MAP in macrophages relies on a diverse series of immunomodulatory mechanisms, including the inhibition of phagosome maturation and apoptosis, generation of cytokine-induced necrosis enabling dissemination of infection through the host, local pathology, and ultimately shedding of the pathogen. Here, we review the bovine macrophage response to infection with M. bovis and MAP. In particular, we describe how recent advances in functional genomics are shedding light on the host macrophage–pathogen interactions that underlie different mycobacterial diseases. To illustrate this, we present new analyses of previously published bovine macrophage transcriptomics data following in vitro infection with virulent M. bovis, the attenuated vaccine strain M. bovis BCG, and MAP, and discuss our findings with respect to the differing etiologies of BTB and JD.

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BoLA class I allele diversity and polymorphism in a herd of cattle

Cited by 21 publications

References 30 publications

Bovine neonatal pancytopenia - Comparative proteomic characterization of two BVD vaccines and the producer cell surface proteome (MDBK)

Bovine neonatal pancytopenia - Comparative proteomic characterization of two BVD vaccines and the producer cell surface proteome (MDBK)

Analysis of MHC class I genes across horse MHC haplotypes

Comparative Functional Genomics and the Bovine Macrophage Response to Strains of the Mycobacterium Genus

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