Bokeloh bat lyssavirus isolation in a Natterer’s bat, Poland

Smreczak, Marcin; Orłowska, Anna; Marzec, Anna; Trębas, Paweł; Müller, Thomas; Freuling, Conrad Martin; Zmudzinski, J.F.

doi:10.1111/zph.12519

Cited by 10 publications

(8 citation statements)

References 12 publications

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“…Daubenton's bat (Myotis daubentonii) is the main reservoir of EBLV-2 [4], and EBLV-2 cases in bats were reported from several European countries [7]. Thus far, lyssaviruses reported from Poland were characterized as EBLV-1 or BBLV [8]. The detection of antibodies against lyssaviruses in two out of three Polish Myotis daubentonii could indicate the presence of EBLV-2 also in Poland, but an interspecies transmission of EBLV-1 from Serotine bats cannot be ruled out either.…”

Section: Discussionmentioning

confidence: 99%

Serological Survey of Lyssaviruses in Polish Bats in the Frame of Passive Rabies Surveillance Using an Enzyme-Linked Immunosorbent Assay

Orłowska

Smreczak

Freuling

et al. 2020

Viruses

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Background: Bats are known to host a number of nonpathogenic viruses, as well as highly pathogenic viruses causing fatal diseases like rabies. Serological surveys as part of active and passive bat rabies surveillance mainly use seroneutralization assays, demonstrating the presence of lyssavirus-specific antibodies in a variety of European bats, particularly against European bat lyssaviruses type 1 (EBLV-1). Here, we present the first serological survey in European bats of this kind during which European bats from Poland collected in the frame of passive rabies surveillance between 2012 and 2018, as well as Serotine bats (Eptesicus serotinus) and North American Big Brown bats (Eptesicus fuscus) from previous experimental studies, were tested using a commercial ELISA kit for the detection of anti-lyssavirus antibodies. Results: Lyssavirus-specific antibodies were detected in 35 (30.4%) out of 115 Polish bats of both sexes, representing nine out of 13 identified bat species endemic mainly to Central Southern Europe and Western Asia, i.e., Eptesicus serotinus, Nyctalus noctula, Myotis daubentonii, Plecotus auritus, Vespertillo murinus,Pipistrellus pipistrellus, Pipistrellus pipilstrellus/Pipistrellus pygmaeus, Myotis brandtii, and Barbastella barbastellus. Seroprevalence was highest in bat species of Nyctalus noctula, Eptesicus serotinus, Plecotus auritus, and Myotis daubentonii. More than 60% of the ELISA seropositive bats originated from the voivodeships of Silesia, Lower-Silesian, Warmian-Mazurian, and Mazowian. Rabies-specific antibodies were also found in Eptesicus fuscus bats from North America. Conclusions: The study demonstrates the principal application of the BioPro Rabies ELISA Ab Kit for the detection of anti-lyssavirus specific antibodies in body fluids and serum samples of bats. However, results may only be reliable for North American bats, whereas interpretation of results for European bats per se is difficult because proper validation of the test is hampered by the protected status of these species.

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Section: Discussionmentioning

confidence: 99%

Serological Survey of Lyssaviruses in Polish Bats in the Frame of Passive Rabies Surveillance Using an Enzyme-Linked Immunosorbent Assay

Orłowska

Smreczak

Freuling

et al. 2020

Viruses

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“…In 2011, Lleida Bat Lyssavirus (LLEBV) was detected also in Miniopterus schreibersii bats in Spain and later on in France [ 105 , 106 ]. Bokeloh bat Lyssavirus (BBLV) was identified in Myotis nattereri in Germany, France, and Poland [ 96 , 99 , 100 ]. Most recently, Kotalahti Bat Lyssavirus (KBLV) was detected in Myotis brandtii in Finland [ 86 , 104 ].…”

Section: European Bat Virusesmentioning

confidence: 99%

Update on Potentially Zoonotic Viruses of European Bats

2021

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Bats have been increasingly gaining attention as potential reservoir hosts of some of the most virulent viruses known. Numerous review articles summarize bats as potential reservoir hosts of human-pathogenic zoonotic viruses. For European bats, just one review article is available that we published in 2014. The present review provides an update on the earlier article and summarizes the most important viruses found in European bats and their possible implications for Public Health. We identify the research gaps and recommend monitoring of these viruses.

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“…Within our study, we identified the tenth BBLV case, which is the seventh case in Germany and the fourth case in Lower Saxony isolated from a Natterer’s bat. Since its first detection in 2010 in Germany [ 12 ], BBLV was found several times in Germany, France and Poland [ 16 , 17 ]. The fact that it was again isolated from the same bat species supports the hypothesis of the Natterer’s bat representing the reservoir host species.…”

Section: Discussionmentioning

confidence: 99%

“…BBLV was first discovered in a Natterer’s bat ( Myotis nattereri ) in Lower Saxony in 2010 [ 12 ]. Ever since, it has been isolated several times from this species in Germany, France and Poland [ 16 , 17 ], suggesting that the Natterer’s bat is the reservoir host species.…”

Section: Introductionmentioning

confidence: 99%

Retrospective Enhanced Bat Lyssavirus Surveillance in Germany between 2018–2020

Calvelage

Schlottau

Hoffmann

et al. 2021

Viruses

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Lyssaviruses are the causative agents for rabies, a zoonotic and fatal disease. Bats are the ancestral reservoir host for lyssaviruses, and at least three different lyssaviruses have been found in bats from Germany. Across Europe, novel lyssaviruses were identified in bats recently and occasional spillover infections in other mammals and human cases highlight their public health relevance. Here, we report the results from an enhanced passive bat rabies surveillance that encompasses samples without human contact that would not be tested under routine conditions. To this end, 1236 bat brain samples obtained between 2018 and 2020 were screened for lyssaviruses via several RT-qPCR assays. European bat lyssavirus type 1 (EBLV-1) was dominant, with 15 positives exclusively found in serotine bats (Eptesicus serotinus) from northern Germany. Additionally, when an archived set of bat samples that had tested negative for rabies by the FAT were screened in the process of assay validation, four samples tested EBLV-1 positive, including two detected in Pipistrellus pipistrellus. Subsequent phylogenetic analysis of 17 full genomes assigned all except one of these viruses to the A1 cluster of the EBLV-1a sub-lineage. Furthermore, we report here another Bokeloh bat lyssavirus (BBLV) infection in a Natterer’s bat (Myotis nattereri) found in Lower Saxony, the tenth reported case of this novel bat lyssavirus.

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Bokeloh bat lyssavirus isolation in a Natterer’s bat, Poland

Cited by 10 publications

References 12 publications

Serological Survey of Lyssaviruses in Polish Bats in the Frame of Passive Rabies Surveillance Using an Enzyme-Linked Immunosorbent Assay

Serological Survey of Lyssaviruses in Polish Bats in the Frame of Passive Rabies Surveillance Using an Enzyme-Linked Immunosorbent Assay

Update on Potentially Zoonotic Viruses of European Bats

Retrospective Enhanced Bat Lyssavirus Surveillance in Germany between 2018–2020

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