Bofutsushosan ameliorates obesity in mice through modulating PGC-1α expression in brown adipose tissues and inhibiting inflammation in white adipose tissues

Chen, Yingying; Yan, Yan; Zhao, Zheng; Shi, Meijing; Zhang, Yubin

doi:10.1016/s1875-5364(16)30042-5

Cited by 7 publications

(7 citation statements)

References 36 publications

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“…Currently, AMH and FC are used to evaluate the development of follicles at different stages under various stimulation conditions [22,23,24]. The results of this study and our previous studies [9] have suggested that cold exposure can affect the metabolism of rats, resulting in reductions in weight gain and damage to the ovarian structure and reserve function, which affects follicular development.…”

Section: Discussionmentioning

confidence: 58%

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Long-term intermittent cold exposure affects peri-ovarian adipose tissue and ovarian microenvironment in rats

Zhang¹,

An²,

Wu³

et al. 2021

Preprint

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Background: This study aimed to investigate the effects of cold exposure on peri-ovarian adipose tissue (POAT) and the relationship between POAT changes and ovarian function, to gain a deeper understanding of the mechanisms associated with cold exposure-induced changes in female reproductive health. Methods: Twenty female rats with regular estrus cycles were randomly divided into two groups: (1) the cold exposure group, which was exposed to –10°C, for 4 hours/day, for two weeks, and (2) the control group, which was maintained at 23 ± 1°C. Results: Cold exposure can increase serum E2 and FSH levels, and reduce AMH hormone levels, and cause the numbers of primary and secondary follicles in the cold-exposed group were significantly higher than those in the control group. Meanwhile, cold-stress-induced browning-related genes, including UCP1, PGC -1a, PRDM16, Fndc5 were upregulated in POAT, and the number of mitochondria in the cold-exposed group was significantly increased. Under cold exposure, the level of adipokines, including APDN, Lep, AMPK were significantly upregulated in POAT. FST was also upregulated in POAT, whereas MST, an AMPK inhibitor, was downregulated. The E2 synthesis rate-limiting-enzymes Cyplla1 and Cyp19 were upregulated in POAT. Conclusion: Cold exposure caused abnormal follicle development and promoted the browning of POAT. POAT browning was accompanied by the activation of the AMPK-PGC1α-Fndc5 pathway.

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Section: Discussionmentioning

confidence: 58%

“…Cold exposure has adverse effects on the female reproductive system [4,5,6,7], affecting ovarian [8] and uterine [4] functions and hormone secretion [9]. However, the exact mechanisms through which these changes occur have not been well-elucidated.…”

Section: Introductionmentioning

confidence: 99%

Long-term intermittent cold exposure affects peri-ovarian adipose tissue and ovarian microenvironment in rats

Zhang¹,

An²,

Wu³

et al. 2021

Preprint

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“…BFT contains ephedrine, which enhances noradrenaline release, and licorice. At high doses, BFT activates UCP-1 expression in the brown adipose tissue and decreases plasma ghrelin levels, suppresses food intake, suppresses body weight gain by decreasing the visceral adipose tissue, and attenuates the progression of steatohepatitis [30][31][32][33][34]37 . However, in our study, we found no effect of BFT on the body weight.…”

Section: Discussionmentioning

confidence: 99%

“…Clinically, BFT exerts a laxative effect since it contains sennosides derived from Rhei rhizoma and has mainly been used in Asian countries for the treatment of constipation in obese patients. It has also been reported that high doses of BFT can activate UCP-1 expression in the brown adipose tissue, decrease the plasma ghrelin level and suppress body weight gain by decreasing the visceral adipose tissue, and attenuate the progression of steatohepatitis [30][31][32][33][34] . However, how BFT affects the gut microbiota and contributes to metabolic improvement is not yet fully understood.…”

mentioning

confidence: 98%

Bofutsushosan improves gut barrier function with a bloom of Akkermansia muciniphila and improves glucose metabolism in mice with diet-induced obesity

Fujisaka

Usui

Nawaz

et al. 2020

Sci Rep

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obesity and insulin resistance are associated with dysbiosis of the gut microbiota and impaired intestinal barrier function. Herein, we report that Bofutsushosan (Bft), a Japanese herbal medicine, Kampo, which has been clinically used for constipation in Asian countries, ameliorates glucose metabolism in mice with diet-induced obesity. A 16S rRNA sequence analysis of fecal samples showed that Bft dramatically increased the relative abundance of Verrucomicrobia, which was mainly associated with a bloom of Akkermansia muciniphila (AKK). Bft decreased the gut permeability as assessed by fitc-dextran gavage assay, associated with increased expression of tight-junction related protein, claudin-1, in the colon. The BFT treatment group also showed significant decreases of the plasma endotoxin level and expression of the hepatic lipopolysaccharide-binding protein. Antibiotic treatment abrogated the metabolic effects of BFT. Moreover, many of these changes could be reproduced when the cecal contents of Bft-treated donors were transferred to antibiotic-pretreated high fat diet-fed mice. These data demonstrate that BFT modifies the gut microbiota with an increase in AKK, which may contribute to improving gut barrier function and preventing metabolic endotoxemia, leading to attenuation of diet-induced inflammation and glucose intolerance. Understanding the interaction between a medicine and the gut microbiota may provide insights into new pharmacological targets to improve glucose metabolism. Obesity and insulin resistance have become epidemic metabolic abnormalities, that can result in pathological complications such as diabetes, hypertension, sarcopenia and cancer 1,2. Obesity-induced insulin resistance is associated with low-grade systemic inflammation, induced by activation of inflammatory signaling 3-7. Thus, controlling obesity-associated inflammation has been a possible therapeutic target in the past couple of decades. The gut microbiota is an important determinant of the metabolic and energy status. They represent a source of energy, such as short-chain fatty acids (SCFAs), that accounts for 5-10% of the total energy demand of the host 8. Hence, germ-free mice are obesity-resistant under a high fat-diet (HFD) and start to gain weight after bacterial

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“…BTS increased mRNA expression levels of leptin, adiponectin, and UCP1 in brown adipose tissues (BAT), and improved insulin resistance, and subsequently reduced serum levels of leptin and triglyceride in parallel with decreased visceral white adipose tissue (WAT) volume and adipocyte size in high-fat diet-fed obese mice [ 3 ]. BTS increased gene expression of PGC-1α and UCP1 for energy consumption in BAT and inhibiting inflammation in WAT in obese mice induced by high-fat diet [ 4 ]. Reduction of subcutaneous and visceral fat and HbA1c was observed in my patient, which may suggest an existence of the same molecular mechanisms in humans.…”

mentioning

confidence: 99%

Bofutsushosan Significantly Ameliorated Betamethasone-Induced Cushing’s Syndrome

Yanai

2018

J Clin Med Res

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Bofutsushosan ameliorates obesity in mice through modulating PGC-1α expression in brown adipose tissues and inhibiting inflammation in white adipose tissues

Cited by 7 publications

References 36 publications

Long-term intermittent cold exposure affects peri-ovarian adipose tissue and ovarian microenvironment in rats

Long-term intermittent cold exposure affects peri-ovarian adipose tissue and ovarian microenvironment in rats

Bofutsushosan improves gut barrier function with a bloom of Akkermansia muciniphila and improves glucose metabolism in mice with diet-induced obesity

Bofutsushosan Significantly Ameliorated Betamethasone-Induced Cushing’s Syndrome

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