Body Temperature Measurements for Metabolic Phenotyping in Mice

Meyer, Carola W.; Ootsuka, Youichirou; Romanovsky, Andrej A.

doi:10.3389/fphys.2017.00520

Cited by 99 publications

(84 citation statements)

References 87 publications

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“…In Dyn-Cre mice, mean ±SEM eye temperature dropped from 36.9°C ±0.3 to 32.8°C ±0.2 with 15 mins of stimulation ( Figure 4H). Thermal imaging to quantify tail temperature can be used to observe heat loss from vasodilation in response to warmth (Meyer et al, 2017).…”

Section: Photostimulation Of Dyn Pbn→poa and Enk Pbn→poa Terminals Camentioning

confidence: 99%

Parabrachial Opioidergic Projections to Preoptic Hypothalamus Mediate Behavioral and Physiological Thermal Defenses

Norris

Shaker

Cone

et al. 2020

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Maintaining stable body temperature through environmental thermal stressors requires detection of temperature changes, relay of information, and coordination of physiological and behavioral responses. Studies have implicated areas in the preoptic hypothalamic area (POA) and the parabrachial nucleus (PBN) as nodes in the thermosensory neural circuitry and indicate the opioid system within the POA is vital in regulating body temperate. In the present study we identify neurons projecting to the POA from PBN expressing the opioid peptides Dynorphin (Dyn) and Enkephalin (Enk). We determine that warm-activated PBN neuronal populations overlap with both Dyn and Enk expressing PBN populations. We demonstrate that Dyn and Enk expressing neurons are partially overlapping subsets of a glutamatergic population in the PBN. Using optogenetic approaches we selectively activate projections in the POA from PBN Dyn, Enk, and VGLUT2 expressing neurons. Our findings demonstrate that Dyn, Enk, and VGLUT2 expressing PBN neurons are critical for physiological and behavioral heat defense. potential roles of opioid receptor mediated behaviors in both Dyn+ and Enk+ PBN-POA projections. Here we report that glutamatergic, Dyn+, and Enk+ neuronal populations projecting from PBN to POA initiate physiological and behavioral heat defensive behaviors. Chemogenetic inhibition of glutamatergic PBN neurons blocks vasomotor responses to thermal heat challenge.The studies reported here provide new insights into the thermoregulatory properties of parabrachial neuropeptide-containing projections to the hypothalamus in homeostatic and metabolic behavior. Results Ambient warmth activates Dyn+ and Enk+ neurons in PBNEffects of mu and kappa receptor signaling on body temperature have been described and mRNA for preprodynorphin and preproenkephalin has been reported to be expressed in the PBN (Baker and Meert, 2002;Chen et al., 2005;Clark, 1979;Engstrom et al., 2001;Hermanson and Blomqvist, 1997;Hermanson et al., 1998). To examine if PBN neurons expressing dynophin or enkephalin opioid neuropeptides are activated by ambient warmth, we exposed mice to ambient warmth (38°C) or room temperature (21-23°C) for four hours prior to preparation of brain for fos staining. We performed immunohistochemistry (IHC) on collected brains sections containing the PBN with antibody directed against c-Fos (anti-c-Fos) to examine induction of c-Fos expression as a marker of neuronal activation (Sheng and Greenberg, 1990). Consistent with recent reports, we observed induction of c-Fos expression in the lateral PBN (LPBN) (Figure 1B-C) (Geerling et al., 2016). In brain sections from warm exposed mice (n=8) compared to room temperature controls (n=4), c-Fos staining revealed a robust and significant (p=0.003) increase in mean ±SEM number of neurons positive for c-Fos expression in the LPBN per brain: 265.8 ±41.9 in warm exposed mice compared to 23.2 ±4.0 in room temperature controls (Figure 1C).Cells in LPBN, lateral to superior cerebellar peduncle, in sections corresponding -5.0 ...

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Section: Photostimulation Of Dyn Pbn→poa and Enk Pbn→poa Terminals Camentioning

confidence: 99%

Parabrachial Opioidergic Projections to Preoptic Hypothalamus Mediate Behavioral and Physiological Thermal Defenses

Norris

Shaker

Cone

et al. 2020

Preprint

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“…Indicate whether used or not. Rectal probe . Apply a bit of lubricant on the probe before inserting Infrared probe (e.g., Braintree Scientific, Braintree, MA, USA).…”

Section: Resultsmentioning

confidence: 99%

“…For more details on the procedure see Appendix . Allow animals to recover for at least 4 days before further experimentation to allow circadian rhythms to normalize (see Meyer et al . and DeBow and Colbourne).…”

Section: Resultsmentioning

confidence: 99%

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A companion to the preclinical common data elements for physiologic data in rodent epilepsy models. A report of the TASK3 Physiology Working Group of the ILAE/AES Joint Translational Task Force

et al. 2018

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SummaryThe International League Against Epilepsy/American Epilepsy Society (ILAE/AES) Joint Translational Task Force created the TASK3 working groups to create common data elements (CDEs) for various aspects of preclinical epilepsy research studies, which could help improve standardization of experimental designs. This article concerns the parameters that can be measured to assess the physiologic condition of the animals that are used to study rodent models of epilepsy. Here we discuss CDEs for physiologic parameters measured in adult rats and mice such as general health status, temperature, cardiac and respiratory function, and blood constituents. We provide detailed CDE tables and case report forms (CRFs), and with this companion manuscript we discuss the monitoring of different aspects of physiology of the animals. The CDEs, CRFs, and companion paper are available to all researchers, and their use will benefit the harmonization and comparability of translational preclinical epilepsy research. The ultimate hope is to facilitate the development of biomarkers and new treatments for epilepsy.

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“…The autonomic nervous system response during such social interactions—highly conserved across mammals and perceived like “stress”—leads to altered vasoactivity in peripheral and core body regions. The resulting blood flow changes via vasoconstriction and vasodilation, respectively, affect local body temperature . Exposure to an embarrassment task, for instance, led to an increase in facial blood flow in both male and female participants, measured via Laser Doppler Flowmetry .…”

Section: Introductionmentioning

confidence: 99%

Vascular response to social cognitive performance measured by infrared thermography: A translational study from mouse to man

et al. 2019

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To assess complex social recognition in mice, we previously developed the SocioBox paradigm. Unexpectedly, 4 weeks after performing in the SocioBox, mice displayed robust social avoidance during Y-maze sociability testing. This unique "sociophobia" acquisition could be documented in independent cohorts. We therefore employed infrared thermography as a non-invasive method of stress-monitoring during SocioBox testing (presentation of five other mice) versus empty box. A higher Centralization Index (body/tail temperature) in the SocioBox correlated negatively with social recognition memory and, after 4 weeks, with social preference in the Y-maze. Assuming that social stimuli might be associated with characteristic thermo-responses, we exposed healthy men (N = 103) with a comparably high intelligence level to a standardized test session including two cognitive tests with or without social component (face versus pattern recognition). In some analogy to the Centralization Index (withinsubject measure) used in mice, the Reference Index (ratio nose/malar cheek temperature) was introduced to determine the autonomic facial response/flushing during social recognition testing. Whereas cognitive performance and salivary cortisol were comparable across human subjects and tests, the Face Recognition Test was associated with a characteristic Reference Index profile. Infrared thermography may have potential for discriminating disturbed social behaviors. K E Y W O R D Sflushing, IRT, social stimulus, stress, temperature, vasoactivity

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Body Temperature Measurements for Metabolic Phenotyping in Mice

Cited by 99 publications

References 87 publications

Parabrachial Opioidergic Projections to Preoptic Hypothalamus Mediate Behavioral and Physiological Thermal Defenses

Parabrachial Opioidergic Projections to Preoptic Hypothalamus Mediate Behavioral and Physiological Thermal Defenses

A companion to the preclinical common data elements for physiologic data in rodent epilepsy models. A report of the TASK3 Physiology Working Group of the ILAE/AES Joint Translational Task Force

Vascular response to social cognitive performance measured by infrared thermography: A translational study from mouse to man

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