Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry

Mei, Jie; Riedel, Nico; Grittner, Ulrike; Endres, Matthias; Banneke, Stefanie; Emmrich, J

doi:10.1038/s41598-018-22020-6

Cited by 64 publications

(73 citation statements)

References 26 publications

(26 reference statements)

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“…4). This phenomenon has been reported as stress from the injection in previous studies 17,18 . Quickly after and for some hours following the AMG2850 injection, SHAM-ANT group showed a significant decrease in Temp of 0.6 °C compared to the other groups during the period from 8:00 to 12:00 am (p < 0.0001).…”

Section: Involvement Of Trpm8 Receptors In the Regulation Of The Bodysupporting

confidence: 62%

Effect of non-thermal radiofrequency on body temperature in mice

Cuc

Delanaud

Bach

et al. 2020

Sci Rep

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Communication technologies based on radiofrequency (RF) propagation bring great benefits to our daily life. However, their rapid expansion raises concerns about possible impacts on public health. At intensity levels below the threshold to produce thermal effects, RF exposure has also recently been reported to elicit biological effects, resembling reactions to cold. The objective of the present study was to investigate the effects of non-thermal RF on body temperature in mice and the related mechanisms. 3-months-old C57BL/6 J mice were exposed to a continuous RF signal at 900 MHz, 20 ± 5 V.m −1 for 7 consecutive days, twice per day during the light phase, for one hour each time. The SAR was 0.16 ± 0.10 W.kg −1. We showed that body temperature patterns in mice change synchronously with the RF exposure periods. Average body temperature in the light phase in the exposed group was higher than in the control group. The expression of the TRPM8 gene was not affected by RF in trigeminal ganglia. Furthermore, the injection of a TRPM8 antagonist did not induce a temperature decrease in exposed mice, as this was the case for sham-controls. These findings indicate that 900 MHz RF exposure at non-thermal level produce a physiological effect on body temperature in mice. However, the involvement of TRPM8 receptors in the mechanism by which RF induced changes in body temperature of mice which remains to be further explored. It must then be assessed if this effect is extrapolable to man, and if this could lead to consequences on health. Radiofrequency (RF) is known to carry insufficient quantum energy to eject electrons from atoms, in contrast to energy of CT scans or X-rays in medical applications. However, RF energy can penetrate the body and induce an elevation of temperature in body tissues as used with the microwave oven technology that is referred to as thermal effects of RF. To protect humans from harmful effects induced by excessive tissue heating, safety limits of RF exposure were established and were described in regulatory guidelines, such as those from ICNIRP (International Commission on Non-Ionizing Radiation Protection) and IEEE (Institute of Electrical and Electronics Engineers) 1. Up to now, except for the microwave auditory effect 2 , the effects of RF at non-thermal levels remain unexplained, poorly reproducible and controversial. In recent years, studies revealed responses induced in rodents by chronic or repeated RF exposure at low intensity which were similar to those observed in cold conditions. Pelletier et al. demonstrated that rats repeatedly exposed (5 weeks, 23 hours per day) to RF at 900 MHz with an intensity of 1 V.m −1 at warm ambient temperature, had a subcutaneous tail temperature 1.2 °C lower than controls, due to maintained vasoconstriction 3. This cannot be explained by a classical dielectric absorption. Also, exposed rats ate more than controls and spent more time to sleep at an ambient temperature of 31 °C, whereas controls spent more time at 28 °C 4. These results led the authors to suggest th...

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Section: Involvement Of Trpm8 Receptors In the Regulation Of The Bodysupporting

confidence: 62%

Effect of non-thermal radiofrequency on body temperature in mice

Cuc

Delanaud

Bach

et al. 2020

Sci Rep

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“…vaccination with EcML. Mice vaccinated with LPS (an unsafe adjuvant) showed hypothermia which lowered the survival rate after challenge [37]. In this study, there was no significantly different change in body temperature in the mice vaccinated using EcML.…”

Section: Discussionmentioning

confidence: 50%

E. coli-Produced Monophosphoryl Lipid a Significantly Enhances Protective Immunity of Pandemic H1N1 Vaccine

et al. 2020

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Emerging influenza viruses pose an extreme global risk to human health, resulting in an urgent need for effective vaccination against influenza infection. Adjuvants are vital components that can improve vaccine efficacy, yet only a few adjuvants have been licensed in human vaccines. Here, we investigate the adjuvant effects of Escherichia coli-produced monophosphoryl lipid A (MPL), named EcML, in enhancing the immunogenicity and efficacy of an influenza vaccine. Similar to MPL, EcML activated dendritic cells and enhanced the antigen processing of cells in vitro. Using ovalbumin (OVA) as a model antigen, EcML increased OVA-specific antibody production, cytotoxic T lymphocyte activity. The safety of EcML was demonstrated as being similar to that of MPL by showing not significant in vitro cell cytotoxicity but transient systemic inflammatory responses within 24 h in OVA immunized mice. Importantly, mice vaccinated with pandemic H1N1 (pH1N1) vaccine antigen, combined with EcML, were fully protected from pH1N1 virus infection by enhanced influenza-specific antibody titers, hemagglutination inhibition titers, and IFN-γ- secreting cells. Taken together, our results strongly suggest that EcML might be a promising vaccine adjuvant for preventing influenza virus infection.

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“…The ΔhvtAΔhupA strain did not grow as well as the ΔhupAΔlacZ strain in this sample. Skin is generally found to be at a lower temperature (34°C in humans, 33.6°C in mice) compared with the spleen and liver (37° in humans, 36.6° in mice; Mei et al, ; Reitman, ). Again, this lower temperature found at a potential wound site could help in early‐stage infections in acquiring hemin from a host, suggesting a role for hvtA in infections at lower temperatures.…”

Section: Discussionmentioning

confidence: 99%

Characterization of temperature‐dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus

Datta

Kenton

2019

MicrobiologyOpen

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The Gram‐negative pathogen Vibrio vulnificus produces several iron‐sequestration systems including a hemin uptake system in response to iron limitation as a means to acquire this essential element. Strains of this organism are capable of causing serious septicemia in humans and eels, where hemin is abundant and an advantageous source of iron. Vibrio vulnificus hemin uptake systems consist of HupA, a well studied outer membrane protein, and a recently identified HvtA protein receptor. In this study, we confirmed that the expression of the hvtA gene is iron‐regulated in a fur‐dependent manner. When analyzed for virulence in a hemin‐overloaded murine model system, the hupA gene was more important for establishing infection than the hvtA gene. Transcriptional profiling of these genes using strains of two different biotypes, biotype 1 (human pathogen) and biotype 2 (eel pathogen), showed that the expression of the two receptors was also regulated in response to temperature. The expression of hupA was highly induced in elevated temperatures in the human pathogenic strain when tested in iron‐depleted conditions. Conversely, hvtA expression was induced significantly in the eel pathogenic strain at a lower temperature, a condition where the hupA locus was relatively repressed. Our results indicate that although both hupA and hvtA are involved for optimal hemin uptake in V. vulnificus, their expression is dually regulated by the environmental cues of iron concentration and temperature. Together, these data suggest that the virulence genes hupA and hvtA are tightly regulated and strictly induced during iron limitation combined with the physiological temperature of the host organism.

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Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry

Cited by 64 publications

References 26 publications

Effect of non-thermal radiofrequency on body temperature in mice

Effect of non-thermal radiofrequency on body temperature in mice

E. coli-Produced Monophosphoryl Lipid a Significantly Enhances Protective Immunity of Pandemic H1N1 Vaccine

Characterization of temperature‐dependent hemin uptake receptors HupA and HvtA in Vibrio vulnificus

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