Body composition, biochemical, behavioral and molecular alterations in overfed rats after chronic exposure to SSRI

Silva, Aline Isabel da; Silva, Severina Cássia de A.; Lima-Júnior, Nelson Correia de; Silva, Tércya Lúcidi de A.; Lagranha, Cláudia Jacques

doi:10.1016/j.bbr.2018.08.007

Cited by 15 publications

(7 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results indicated that the SNS group administered with long-term chronic restraint stress showed a significant increase in BW gain compared with the stress group (25). However, the absence of effect of fluoxetine on the loss of weight in MS rats may be attributed to the enhanced satiety for food and restrain hunger-related pathways, as previously reported (26).…”

Section: Discussionsupporting

confidence: 63%

SiNiSan Ameliorates the Depression-Like Behavior of Rats That Experienced Maternal Separation Through 5-HT1A Receptor/CREB/BDNF Pathway

et al. 2019

View full text Add to dashboard Cite

Background: Early adverse life stress is an important dangerous factor in the development of psychiatric disorders, particularly depression. Available clinical antidepressant agents, such as fluoxetine, [a selective serotonin reuptake inhibitor (SSRI)], are unsatisfactory because of their side effects. SiNiSan (SNS) is a classic Chinese medicine prescription regarded to disperse stagnated liver qi to relieve qi stagnation. Therefore, this study was designed to detect the effects and molecular mechanism of SNS treatment in rats subjected to maternal separation (MS). Method: Male neonatal Wistar rats were divided into six groups including control + ddH 2 O, MS + ddH 2 O, MS + fluoxetine (5 g/kg), MS + SNS -low dose (2.5 g/kg), MS + SNS -medium dose (5 g/kg), MS + SNS -high dose (10 g/kg). The volume of drugs and ddH2O in each group are according to the weight of rats every day (10 mL/kg). Each group comprised 16 pups with 8 young and 8 adult pups. Except for the control group, all MS groups were separated from their mothers for 4 h/day from 9:00 to 13:00 during postnatal days (PNDs) 1 to 21. After MS, the six groups were intragastrically administered with ddH2O, fluoxetine, and different doses of SNS until PND 28 (for young pups) and PND 56 (for adult pups). The pups were weighed every day, and depression-like behavior was assessed by sucrose preference test, open field test, and forced swimming test. Serotonin 1A (5-HT1A) receptor, phosphorylated protein kinase A (p-PKA) substrate, cAMP response element-binding protein (CREB), p-CREB and brain-derived neurotrophic factor (BDNF) in the hippocampus were examined by Western blot, and in situ 5-HT1A receptor expression was measured by IHC. Results: Young and adult MS rats exhibited depression-like behavior. However, the depression-like behavior was ameliorated by SNS in both age groups. The levels of 5-HT1A receptor, p-CREB, and BDNF in the hippocampus were reduced in young and adult MS rats. SNS treatment significantly up-regulated the expression of 5-HT1A receptor, p-CREB, and BDNF in the hippocampus of adult MS rats. However, few significant effects on the protein expression were observed in the young MS rats. Conclusion: MS in infancy could develop depression-like behavior in young and adult. SNS treatment may perform antidepressant effects on young and adult MS rats through the BDNF/PKA/CREB pathway.

show abstract

Section: Discussionsupporting

confidence: 63%

SiNiSan Ameliorates the Depression-Like Behavior of Rats That Experienced Maternal Separation Through 5-HT1A Receptor/CREB/BDNF Pathway

et al. 2019

View full text Add to dashboard Cite

show abstract

“…On a postnatal day 3 (PND3), groups were divided to adjust litter size: the Normofed Group (N, n = 9 pups per litter) and the Overfed Group (O, n = 3 pups per litter). The reduced litter size increases the food/nutrient supply for the offspring, as previously described by Plagemann et al (2009) and other researchers (Ferreira et al, 2018; Silva et al, 2019).…”

Section: Methodssupporting

confidence: 67%

Effects of fluoxetine withdrawal in the brainstem and hypothalamus of overnourished rats: Chronic modulation on oxidative stress levels

Santana

Rodrigues

Lima

et al. 2023

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

Poor nutritional quality in the early stages of development is associated with neurological diseases in adulthood. Studies showed that obesity‐induced oxidative stress contributes to the genesis of neurological diseases through dysregulation of the brainstem and hypothalamus. Fluoxetine (Fx) is an antidepressant member in the family of selective serotonin reuptake inhibitors (SSRI) that can induce positive effects by reducing oxidative damage in brain tissues. We aimed to evaluate the late effect of Fx in the brainstem and hypothalamus of overnourished rats during development. Male Wistar rats, after birth, were randomly divided into the normal‐nourished group (N, n = 9) and the overnourished group (O, n = 3). On the 39th day of life, the groups were subdivided into normofed, and the overnourished group treated or not with fluoxetine (10 mg/kg daily) (NF, NV, OF, and OV). All groups were treated from the 39th to the 59th day of life, and within 90 days, the tissues were collected for oxidative stress analysis. Briefly, our results showed that Fx treatment induced a tissue‐dependent long‐lasting effect in overfed animals, increasing the enzymatic defense (i.e., CAT and GST activity) in the hypothalamus, but more intensive, increasing the non‐enzymatic defense (i.e., Total Thiols and GSH levels) in the brainstem. Overall, our study suggests that serotonin modulation at the final stage of brain development causes a long‐lasting impact on brain structures in overfed rats at a different mode.

show abstract

“…5‐HT 1B stimulation reduces neuropeptide Y (NPY)/agouti‐related peptide (AgRP) neuronal activity and therefore tonic GABAergic inhibition of proopiomelanocortin (POMC)/cocaine and amphetamine‐regulated transcript (CART) neurons, which in turn are activated by 5‐HT 2C stimulation. These circuits synergistically contribute to the anorectic effects of serotonergic receptors in the hypothalamus (da Silva et al, ). In animal studies, it was suggested that chronic paroxetine treatment led to a decrease in body weight (Amodeo et al, ; de Jong et al, ; Konkle & Bielajew, ; Konkle, Sreter, Baker, & Bielajew, ).…”

Section: Discussionmentioning

confidence: 99%

Effects of long‐term paroxetine or bupropion treatment on puberty onset, reproductive and feeding parameters in adolescent male rats

et al. 2019

View full text Add to dashboard Cite

Antidepressant use in adolescents has become more common in recent years. We have found several studies stating that prenatal antidepressant exposure can lead to delayed or earlier puberty onset but there was no study on postnatal paroxetine or bupropion. The main aim of this study was to investigate the effect of postnatal exposure to bupropion or paroxetine on puberty onset, reproductive and feeding results. The male rats (n = 8/group) aged 21 days were exposed to paroxetine (3.6 mg/kg) or bupropion (17 mg/kg) orally by gastric gavage every day from postnatal day 21–90. Also, control group received only saline orally as a vehicle. Postnatal exposure to bupropion or paroxetine delayed puberty onset compared to control group, but it was not significant. Sperm counts were significantly lower in the paroxetine and bupropion groups compared to control group. Sperm motility was significantly lower in only bupropion group. In addition, sperm motility was lower in paroxetine group, but it was not significant. In the histopathological examination, there was damage to the testicular structure in both treatments. Taken together, our result indicates that postnatal paroxetine or bupropion exposure may affect puberty onset and contribute to the impairment in fertility in male rats.

show abstract

Body composition, biochemical, behavioral and molecular alterations in overfed rats after chronic exposure to SSRI

Cited by 15 publications

References 48 publications

SiNiSan Ameliorates the Depression-Like Behavior of Rats That Experienced Maternal Separation Through 5-HT1A Receptor/CREB/BDNF Pathway

SiNiSan Ameliorates the Depression-Like Behavior of Rats That Experienced Maternal Separation Through 5-HT1A Receptor/CREB/BDNF Pathway

Effects of fluoxetine withdrawal in the brainstem and hypothalamus of overnourished rats: Chronic modulation on oxidative stress levels

Effects of long‐term paroxetine or bupropion treatment on puberty onset, reproductive and feeding parameters in adolescent male rats

Contact Info

Product

Resources

About