BoCAPS: Rapid screening of chemical races in Botryococcus braunii with direct PCR-CAPS

Kawamura, Kôji; Nishikawa, Suzune; Hirano, Kotaro; Ardianor, Ardianor; Nugroho, Rudy Agung

doi:10.1016/j.algal.2022.102789

Cited by 1 publication

(1 citation statement)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a consequence of the different hydrocarbons, each race has a distinct ECM composition with the hydrocarbons in each race linked by epoxide bridges to long-chain polyacetals spanning the ECM. Phylogeny studies focused on the 18S rRNA gene revealed distinct clade-level groupings for each of the three races [ 9 , 16 , 17 , 20 , 23 ]. Despite these many differences, B .…”

Section: Introductionmentioning

confidence: 99%

Reclassification of Botryococcus braunii chemical races into separate species based on a comparative genomics analysis

Boland,

Cornejo-Corona,

Browne

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

The colonial green microalga Botryococcus braunii is well known for producing liquid hydrocarbons that can be utilized as biofuel feedstocks. B. braunii is taxonomically classified as a single species made up of three chemical races, A, B, and L, that are mainly distinguished by the hydrocarbons produced. We previously reported a B race draft nuclear genome, and here we report the draft nuclear genomes for the A and L races. A comparative genomic study of the three B. braunii races and 14 other algal species within Chlorophyta revealed significant differences in the genomes of each race of B. braunii. Phylogenomically, there was a clear divergence of the three races with the A race diverging earlier than both the B and L races, and the B and L races diverging from a later common ancestor not shared by the A race. DNA repeat content analysis suggested the B race had more repeat content than the A or L races. Orthogroup analysis revealed the B. braunii races displayed more gene orthogroup diversity than three closely related Chlamydomonas species, with nearly 24-36% of all genes in each B. braunii race being specific to each race. This analysis suggests the three races are distinct species based on sufficient differences in their respective genomes. We propose reclassification of the three chemical races to the following species names: Botryococcus alkenealis (A race), Botryococcus braunii (B race), and Botryococcus lycopadienor (L race).

show abstract