Board 6: Work in Progress: Alternative Lab Reports for Biomedical Engineering

Jensen, Karin; Jensen, Paul A.

doi:10.18260/1-2--32387

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…Students are instructed on data processing to obtain the pGLO CN. Student assessments include a written laboratory report where students validate features and CN of the pGLO plasmid ( 13 ).…”

Section: Resultsmentioning

confidence: 99%

Laboratory Exercise to Measure Plasmid Copy Number by qPCR

David

Masood

et al. 2021

J Microbiol Biol Educ.

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Quantitative PCR (qPCR) has numerous applications in biology. In an education setting, qPCR provides students an opportunity to better understand the PCR mechanism by providing both quantitative information about the reactions and also data to troubleshoot PCRs (e.g., melt curves). Here, we present a relatively short (2-h) laboratory activity to demonstrate qPCR to quantify plasmid copy number (CN) by measuring the cycle threshold ( C T ) values for a genomic gene and a plasmid gene using transformed cells as a template. The activity can be combined with additional laboratory exercises, including bacterial transformation, to create the template to be used in the qPCRs. This lab activity is ideal for undergraduate laboratory courses that include recombinant DNA technology.

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Section: Resultsmentioning

confidence: 99%

Laboratory Exercise to Measure Plasmid Copy Number by qPCR

David

Masood

et al. 2021

J Microbiol Biol Educ.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Course Design Cell and Tissue Engineering Lab is a two-credit core course for second-year students in the bioengineering major at the University of Illinois Urbana-Champaign [8] and was offered both in-person and online during the Fall of 2020. Online students were provided with a kit that contained micropipettes, digital scale, glucose meter, lab consumables, 3D printed gel casting mold and comb, and reagents to perform a BCA and an enzyme assay.…”

Section: Methodsmentioning

confidence: 99%