Boar variability affects sperm metabolism activity in liquid stored semen at 5°C

Dziekońska, Anna; Strzeżek, J.

doi:10.2478/v10181-011-0003-1

Cited by 24 publications

(22 citation statements)

References 26 publications

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“…Moreover, motility has been demonstrated to be highly correlated with mitochondrial function of boar spermatozoa following liquid semen storage (Fraser et al 2003, Trzcińska et al 2008, Dziekońska et al 2009, Dziekońska and Strzeżek 2011. In the present study differences in the proportions of spermatozoa with high MMP between the extenders were more marked during prolonged storage, particularly in the AeG and SCP extenders.…”

Section: Discussionsupporting

confidence: 52%

“…Also, individual differences between the boars could affect the quality of the liquid-stored semen (Dziekońska and Strzeżek 2011). However, in the current study there were not any marked differences between the three boars, with respect to the analyzed sperm metabolic activity and membrane integrity, during prolonged storage at 17 o C. Taken together, the finding of this study strongly suggest that the type of extender selected for long-term storage of boar spermatozoa is important in order to increase the semen longevity with minimum deterioration in the sperm function.…”

Section: Sperm Membrane Integritymentioning

confidence: 99%

“…Besides sperm motility assessments, the sperm mitochondrial function can be monitored with a cationic fluorescent probe, 5,5',6,6'-tetrachloro--1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1), which accumulates in the mitochondria, depending on the mitochondrial membrane potential (MMP), and the measurements of adenosine triphosphate (ATP) content (Thomas et al 1998, Dziekońska et al 2009, Gogol et al 2009). Both MMP and ATP production are indispensable for flagellar beat and motility of spermatozoa (Guthrie et al 2008, Storey 2008, Waberski et al 2011, and have been used to assess the sperm metabolic activity in liquid-stored boar semen (Huo et al 2002, Fraser et al 2003, Trzcińska et al 2008, Dziekońska et al 2009, Dziekońska and Strzeżek 2011. Furthermore, the plasma membrane integrity (PMI) and the acrosome integrity are essential for cell viability, and their maintenance during prolonged liquid semen storage is crucial for the events leading to fusion with the oocyte (Flesch and Gadella 2000).…”

Section: Introductionmentioning

confidence: 99%

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Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17°C

Dziekońska¹,

Fraser²,

Majewska³

et al. 2013

Polish Journal of Veterinary Sciences

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This study was aimed to analyze the metabolic activity and membrane integrity of boar spermatozoa following storage in long-term semen extenders. Boar semen was diluted with Androhep ® EnduraGuard TM (AeG), DILU-Cell (DC), SafeCell Plus TM (SCP) and Vitasem LD (VLD) extenders and stored for 10 days at 17 o C. Parameters of the analyzed sperm metabolic activity included total motility (TMOT), progressive motility (PMOT), high mitochondrial membrane potential (MMP) and ATP content, whereas those of the membrane integrity included plasma membrane integrity (PMI) and normal apical ridge (NAR) acrosome. Extender type was a significant (P < 0.05) source of variation in all the analyzed sperm parameters, except for ATP content. Furthermore, the storage time had a significant effect (P < 0.05) on the sperm metabolic activity and membrane integrity during semen storage. In all extenders the metabolic activity and membrane integrity of the stored spermatozoa decreased continuously over time. Among the four analyzed extenders, AeG and SCP showed the best performance in terms of TMOT and PMI on Days 5, 7 and 10 of storage. Marked differences in the proportions of spermatozoa with high MMP were observed between the extenders, particularly on Day 10 of storage. There were not any marked differences in sperm ATP content between the extenders, regardless of the storage time. Furthermore, the percentage of spermatozoa with NAR acrosomes decreased during prolonged storage, being markedly lower in DC-diluted semen compared with semen diluted with either AeG or SCP extender. The results of this study indicated that components of the long-term extenders have different effects on the sperm functionality and prolonged semen longevity by delaying the processes associated with sperm ageing during liquid storage.

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Section: Discussionsupporting

confidence: 52%

Section: Sperm Membrane Integritymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17°C

Dziekońska¹,

Fraser²,

Majewska³

et al. 2013

Polish Journal of Veterinary Sciences

Self Cite

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“…Studies on fresh and stored boar semen using variants of the bioluminescence method report variable ATP contents ranging between 5 and 152 pmol/10 6 sperm (Long and Guthrie, 2006; Dziekońska and Strzeżek, 2011; Dziekońska et al, 2013). Ideally, an ATP assay should allow detection of all intracellular ATP.…”

Section: Introductionmentioning

confidence: 99%

Energy metabolic state in hypothermically stored boar spermatozoa using a revised protocol for efficient ATP extraction

et al. 2016

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Mammalian spermatozoa utilize ATP as the energy source for key functions on the route to fertilization. ATP and its precursor nucleotides ADP and AMP are regularly investigated in sperm physiology studies, mostly by bioluminescence assays. Assay results vary widely, mainly due to different efficiencies in nucleotide extraction and prevention of their enzymatic degradation. Here, we describe a revised, validated protocol for efficient phosphatase inhibition and adenine nucleotide extraction resulting in consistently high ATP concentrations exceeding previously reported values for boar spermatozoa up to 20-fold. The revised assay is applicable for determining ATP concentrations and adenylate energy charge in extracts from fresh and frozen samples, thereby allowing simultaneous assessment of semen samples from long-term storage experiments. After validation, the assay was applied to liquid-preserved boar spermatozoa stored at 17°C and 5°C for 24 and 72 h. Cooling to 5°C, but not storage duration, reduced ATP concentration in spermatozoa (P<0.05), which was accompanied by the appearance of AMP and ADP in the preservation medium. ATP and energy charge were highly correlated to the proportion of membrane-intact spermatozoa, supporting the idea of nucleotides leaking through disrupted membranes in cold-shocked cells. The present assay allows highly standardized studies of energy metabolism in spermatozoa.

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“…In the contemporary technology of conventional pig AI, diluted liquid semen doses of 80 ml to 100 ml are used, which contain 3-5 × 10 9 progressively motile spermatozoa, and which are stored at 17 °C for zero to five days (Johnson et al 2000;Haugan et al 2005;Alm et al 2006;Stancic et al 2009;Khalifa et al 2014). However, the fertilising potential of extended boar semen declines within the first 72 h of in vitro storage (Dziekonska and Strzezek 2011;Waberski et al 2011). In modern intensive pig production, the 1200 average of annual insemination doses produced per boar is inefficient due to the high cost of genetically doi: 10.17221/8720-VETMED high quality boars (Singleton 2001;Stancic et al 2009).…”

mentioning

confidence: 99%

Motility of boar spermatozoa supplemented with homologous seminal plasma of high or low protein content after storage for three days

Apić¹,

Stančić²,

Vakanjac³

et al. 2016

Vet. Med. - Czech

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ABSTRACT:The effect of adding high or low protein content homologous seminal plasma to boar spermatozoa on the progressive motility of the spermatozoa after storage for three days, and at a 1 : 4 dilution ratio was investigated. A total of 32 ejaculates collected from four boars (eight ejaculates per boar) with high seminal plasma protein content (4%, HH-group) and 32 ejaculates collected from four boars (eight ejaculates per boar) with low seminal plasma protein content (2%, LL-group) were evaluated. The fresh ejaculate samples were centrifuged at 1000 g for 10 min at 4 °C to separate the spermatozoa from the seminal plasma. After centrifugation, one of the centrifuged spermatozoa samples was used to form an autologous fresh ejaculate sample, and another to form a homologous sample by adding low or high protein content seminal plasma from other boars. It was found that semen samples formed with spermatozoa from HH-group boars, supplemented with seminal plasma from LL-group, boars have significantly (P < 0.01) lower progressive motility (55%) after storage for 72 h than samples containing the boar's own (autologous) seminal plasma (65%). Conversely, when homologous seminal plasma with high protein content was added to the spermatozoa isolated from the boar ejaculate with low protein content in its seminal plasma, progressive motility significantly (P < 0.01) increased from 52% in samples with autologous seminal plasma, to 65% in samples with homologous seminal plasma. It was concluded that addition of homologous high protein content seminal plasma to the spermatozoa of boars with low protein content in their seminal plasma increases their progressive motility after storage for 72 h at a 1 : 4 dilution ratio. This could be a useful tool for increasing reproductive performance in lower fertility high genetic quality boars.

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Boar variability affects sperm metabolism activity in liquid stored semen at 5°C

Cited by 24 publications

References 26 publications

Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17°C

Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17°C

Energy metabolic state in hypothermically stored boar spermatozoa using a revised protocol for efficient ATP extraction

Motility of boar spermatozoa supplemented with homologous seminal plasma of high or low protein content after storage for three days

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