Boar spermatozoa as a biosensor for detecting toxic substances in indoor dust and aerosols

Andersson, Maria; Mikkola, Raimo; Rasimus, Stiina; Hoornstra, Douwe; Salin, Pekka Johannes; Rahkila, R.; Heikkinen, Maire S.A.; Mattila, Sampo; Peltola, J.; Kalso, S.; Salkinoja‐Salonen, Mirja

doi:10.1016/j.tiv.2010.08.011

Cited by 32 publications

(53 citation statements)

References 45 publications

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“…The boar sperm motility inhibition assay, including all the controls for aging and vehicle-exposed cells and for the repeatability of the assay, was executed as described previously (2). The change in the mitochondrial potential (⌬⌿ m ) and the change in the plasma membrane potential (⌬⌿ p ) in boar spermatozoa were recorded by use of the fluorogenic dye JC-1 (5,5=,6,6=-tetrachloro-1,1=,3,3=-tetraethylbenzimidazolylcarbocyanine iodide) as described previously by Hoornstra et al (27).…”

Section: Or 50 G Of Nacl Litermentioning

confidence: 99%

Psychrotolerant Paenibacillus tundrae Isolates from Barley Grains Produce New Cereulide-Like Depsipeptides (Paenilide and Homopaenilide) That Are Highly Toxic to Mammalian Cells

Rasimus

Mikkola

Andersson

et al. 2012

Appl Environ Microbiol

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Section: Or 50 G Of Nacl Litermentioning

confidence: 99%

Psychrotolerant Paenibacillus tundrae Isolates from Barley Grains Produce New Cereulide-Like Depsipeptides (Paenilide and Homopaenilide) That Are Highly Toxic to Mammalian Cells

Rasimus

Mikkola

Andersson

et al. 2012

Appl Environ Microbiol

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“…Thus, the change of sperm motility is able to sensitively indicate the substances causing mitochondrial membrane depolarization even at sublethal doses, which is undetectable with other cell lines [3]. Motility of the spermatozoa was judged after exposure of 30 min and 24 h at room temperature with minor modification of a previously described method [23,24]. OPA inhibited sperm motility after one day of exposure at 1/10 of the concentration giving inhibition visible already after 30 min, because ≤2.5 μg mL −1 and 250 ng mL −1 concentration values were determined as endpoints (EC 50 ) for short and long term exposure, respectively (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…The sperm motility inhibition assay was done as reported by Andersson et al [24]. Commercial boar semen obtained from Figen Oy Finland extended in MR-A (Kubus, S.A., Madrid, Spain) containing 27 × 10 6 spermatozoa·mL −1 was used.…”

Section: Methodsmentioning

confidence: 99%

Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane Integrities of Porcine Sperm and Induces Cell Death on Mammalian Somatic Cell Lines

Bencsik

Papp

Berta

et al. 2014

Toxins

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Bipolaris oryzae is a phytopathogenic fungus causing a brown spot disease in rice, and produces substance that strongly perturbs motility and membrane integrities of boar spermatozoa. The substance was isolated from the liquid culture of the fungal strain using extraction and a multi-step semi-preparative HPLC procedures. Based on the results of mass spectrometric and 2D NMR techniques, the bioactive molecule was identified as ophiobolin A, a previously described sesterterpene-type compound. The purified ophiobolin A exhibited strong motility inhibition and viability reduction on boar spermatozoa. Furthermore, it damaged the sperm mitochondria significantly at sublethal concentration by the dissipation of transmembrane potential in the mitochondrial inner membrane, while the plasma membrane permeability barrier remained intact. The study demonstrated that the cytotoxicity of ophiobolin A toward somatic cell lines is higher by 1–2 orders of magnitude compared to other mitochondriotoxic mycotoxins, and towards sperm cells unique by replacing the progressive motility by shivering tail beating at low exposure concentration.

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“…The effects of toxins on boar sperm motility were investigated by using the boar sperm motility assay as described previously (24).…”

Section: Methodsmentioning

confidence: 99%

“…These culture suspensions were then exposed to the test substances at concentrations from 0.001 g to 100 g ml Ϫ1 TSB. Prior to being added to the culture suspensions, the pure substances were dissolved in methanol and heated in a water bath (80°C) for 10 min, and the bioactivity of the methanol solutions was verified in the boar sperm motility assay, using a 30-min exposure time as described previously (24). TSB with the methanol extracts of the test substances only was used as a sterility control of the test substances, and pure methanol, without a target strain, was used as an internal negative control (with no bacterial growth indicated by no increase in turbidity).…”

Section: Methodsmentioning

confidence: 99%

The Peptide Toxin Amylosin of Bacillus amyloliquefaciens from Moisture-Damaged Buildings Is Immunotoxic, Induces Potassium Efflux from Mammalian Cells, and Has Antimicrobial Activity

Rasimus-Sahari

Теплова

Andersson

et al. 2015

Appl Environ Microbiol

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f Amylosin, a heat-stable channel-forming non-ribosomally synthesized peptide toxin produced by strains of Bacillus amyloliquefaciens isolated from moisture-damaged buildings, is shown in this paper to have immunotoxic and cytotoxic effects on human cells as well as antagonistic effects on microbes. Human macrophages exposed to 50 ng of amylosin ml ؊1 secreted high levels of cytokines interleukin-1␤ (IL-1␤) and IL-18 within 2 h, indicating activation of the NLRP3 inflammasome, an integral part of the innate immune system. At the same exposure level, expression of IL-1␤ and IL-18 mRNA increased. Amylosin caused dose-dependent potassium ion efflux from all tested mammalian cells (human monocytes and keratinocytes and porcine sperm cells) at 1 to 2 M exposure. Amylosin also inhibited the motility of porcine sperm cells and depolarized the mitochondria of human keratinocytes. Amylosin may thus trigger the activation of the NLRP3 inflammasome and subsequently cytokine release by causing potassium efflux from exposed cells. The results of this study indicate that exposure to amylosin activates the innate immune system, which could offer an explanation for the inflammatory symptoms experienced by occupants of moisture-damaged buildings. In addition, the amylosin-producing B. amyloliquefaciens inhibited the growth of both prokaryotic and eukaryotic indoor microbes, and purified amylosin also had an antimicrobial effect. These antimicrobial effects could make amylosin producers dominant and therefore significant causal agents of health problems in some moisture-damaged sites.A mylosin is a heat-stable 1,197-Da peptide toxin originally found to be produced by strains of Bacillus amyloliquefaciens isolated from moisture-damaged buildings (1, 2). B. amyloliquefaciens belongs to the Bacillus subtilis group, members of which have been found in high numbers in moisture-damaged buildings where occupants have experienced building-related ill health symptoms (3, 4). Amylosin forms cation-permeant ion channels with high selectivity for K ϩ in lipid membranes (5) and thus depolarizes the plasma membrane and the mitochondria inside live mammalian cells, disrupting cellular ion homeostasis, mitochondrial functions, and energy metabolism (1, 2). It has been suggested that amylosin causes health risks for people living or working in moisture-damaged buildings contaminated with amylosin-producing bacteria (1). Amylosin has also been shown to be produced by B. subtilis and Bacillus mojavensis strains connected to foodborne illness (6).Moisture-damaged buildings harbor many kinds of fungi and bacteria, including producers of bioactive compounds (7, 8; reviewed in reference 9). The symptoms experienced by people living or working in moisture-damaged buildings vary (10), but they could at least partly result from activation of the innate immune system (11,12). Symptoms such as acute inflammatory responses and pseudoallergic reactions may be a result of the activation of inflammasomes (12,13). Inflammasomes are components of the innate immun...

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Boar spermatozoa as a biosensor for detecting toxic substances in indoor dust and aerosols

Cited by 32 publications

References 45 publications

Psychrotolerant Paenibacillus tundrae Isolates from Barley Grains Produce New Cereulide-Like Depsipeptides (Paenilide and Homopaenilide) That Are Highly Toxic to Mammalian Cells

Psychrotolerant Paenibacillus tundrae Isolates from Barley Grains Produce New Cereulide-Like Depsipeptides (Paenilide and Homopaenilide) That Are Highly Toxic to Mammalian Cells

Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane Integrities of Porcine Sperm and Induces Cell Death on Mammalian Somatic Cell Lines

The Peptide Toxin Amylosin of Bacillus amyloliquefaciens from Moisture-Damaged Buildings Is Immunotoxic, Induces Potassium Efflux from Mammalian Cells, and Has Antimicrobial Activity

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